JI Liang,WANG Xian,PENG Lin,et al.Effects of Nitrogen Ion Beam Implantation and 60Co-γ Irradiation on Yield Traits and Quality of Soybean[J].Soybean Science,2013,32(02):189-192.[doi:10.3969/j.issn.1000-9841.2013.02.011]
氮离子束注入和钴60伽玛辐射对大豆产量性状和品质的影响
- Title:
- Effects of Nitrogen Ion Beam Implantation and 60Co-γ Irradiation on Yield Traits and Quality of Soybean
- Keywords:
- Soybean; Nitrogen ion beam implantation; 60Co-γ ray irradiation; Yield traits; Quality; Mutation breeding
- 文献标志码:
- A
- 摘要:
- 分别以3种剂量的N离子束注入(4×1016，6×1016和8×1016 N+·cm-2)和3种剂量的60Co-γ射线辐射(100，150和200 Gy)处理4份大豆干种子，在相同种植条件下对其M6代产量性状和品质进行研究，比较2种处理方法对大豆产量性状及品质的诱变效应。结果表明：与对照相比，新大豆3号4×1016 N+.cm-2离子束注入处理单株荚数显著增加，6×1016 N+·cm-2离子束注入处理产量极显著增加；合99-756 8×1016N+·cm-2离子束注入处理单株粒数和单株粒重显著减少，6×1016N+·cm-2离子束注入处理百粒重显著减少，100 Gy 60Co-γ射线辐射处理蛋白质含量显著降低，100,150和200 Gy 60Co-γ射线辐射使脂肪含量显著提高；新大豆8号 4×1016和8×1016N+·cm-2离子束注入、100和200 Gy 60Co-γ射线辐射处理单株粒重显著减少，4×1016和6×1016 N+·cm-2离子束注入及200 Gy 60Co-γ射线辐射处理产量极显著或显著增加;N离子束注入和60Co-γ辐射对石大豆2号产量、各产量构成因素及品质的影响差异不显著。因此，诱变育种目标应根据材料的敏感性、诱变方法和剂量综合确定。
- Abstract:
- Selection of materials,mutagenic methods and doses are very important in mutation breeding.The objectives of this experiment was to discuss the mutation breeding technique of soybean by nitrogen ion beam implantation and 60Co-γ ray radiation.The dried seeds of four soybean varieties were treated by nitrogen ion beam implantation including 4×1016，6×1016和8×1016 N+·cm-2 and 60Co-γ ray irradiation of 100,150 and 200 Gy.The biological effects of two treatments on yield traits and quality of M6 generation were compared under the same planting conditions.The results indicated that compared with control，4×1016 and 6×1016 N·cm-2 increased pods per plant(P<0.05) and yield(P<0.01)of Xindadou 3 significantly;8×1016,6×1016and 100 Gy 60Co-γ irradiation decreased seeds number and weight per plant(P<0.05),100seed weight(P<0.05)and protein content of He99756,respectively.100,150 and 200 Gy 60Co-γ ?irradiation increased fat content(P<0.05)of He99-756;4×1016 and 8×1016 N+·cm-2,100 and 200 Gy 60Co-γ irradiation decreased seeds weight per plant(P<0.05)of Xindadou 8,4×1016,6×1016 N+.cm-2 and 200 Gy 60Co-γ?irradiation increased yield of Xindadou 8 significantly;nitrogen ion beam implantation and 60Co-γ irradiation had no effect on the yield,yield components and quality of Shidadou 2.Thus the objectives of mutation breeding were determined according to sensitivity of materials,mutagenic methods and doses.
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备注/Memo
?新疆高技术研究发展计划项目（201111118）。