[1]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33-42.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(01):33-42.[doi:10.3969/j.issn.1000-9841.2013.01.008]
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大豆“冀黄13”突变体筛选及突变体库的建立

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第32卷 期数: 2013年01期 页码: 33-42 栏目: 出版日期: 2013-02-25
Title:
Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”
文章编号:
1000-9841(2013)01-0033-05
作者:
张力伟1; 樊颖伦2; 3; 牛腾飞1; 张文会1; 赵玉洁1; 刘立科1; 3
1.聊城大学 生命科学学院，山东 聊城 252059；
2.聊城大学 农学院，山东 聊城 252059;
3.聊城大学 农作物育种研究所，山东 聊城 252059
Author(s):
ZHANG Li-wei 1;  FAN Ying-lun 2; 3;  NIU Teng-fei? 1;  ZHANG Wen-hui1;  ZHAO Yu-jie1;  LIU Li-ke 1; 3
1.School of Life Science，Liaocheng University;
2.School of Agricultural Science,Liaocheng University;
3.Institute of Crop Breeding,Liaocheng University,Liaocheng 252059,Shandong,China
关键词:
大豆; 理化诱变; 冀黄13
Keywords:
Soybean; Physical and chemical mutagenesis; Jihuang13
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2013.01.008
摘要:
利用甲基磺酸乙酯(ethyl methane sulfonate,EMS)、叠氮化钠(sodium azide,NaN3)和N离子束分别诱变处理大豆品种"冀黄13"的种子。经M2选择,M3、M4代验证,共筛选出茎器官突变体15份,叶器官突变体19份,花器官突变体31份,种子器官突变体3份以及成熟期突变体18份,其中有9份突变体属于复合性状的突变,最终获得77份稳定遗传的突变体。通过F2群体的表型分析,确定1份雄性不育突变体和1份黑种皮色突变体的表型均由单隐性基因控制。本研究所获得的突变体和所构建突变体库将为大豆功能基因组学研究和遗传改良奠定基础。
Abstract:
Mutants and mutant population are the foundation for gene function analysis and crop genetic improvement.Seed of soybean cultivar “Jihuang 13” were treated with ethyl methane sulfonate(EMS),sodium azide(NaN3)and nitrogen ion beam implantation respectively for screening mutants and constructingmutant population.Fifteen stem related mutants,19 leaf related mutants,31 floral related mutants,3 seed related mutants,and 18 maturity related mutants were obtained after selected in M2generation,and validated in M3and M4 generation.Because there were 9 mutants possessing composite phenotypes,77 genetically stable mutants were finally screened out.Further genetic analyses for a male sterile mutant and a black seed color mutant were made in F2 segregating population respectively.The results showed that the phenotypes of these two mutants were both controlled by a single recessive gene.The mutants and mutant population obtained would lay a foundation for functional genomics analyses and genetic improvement of soybean.

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备注/Memo

基金项目：国家自然科学基金（31071436）。

第一作者简介：张力伟（1989-），男，在读硕士，研究方向为生物催化和生物转化研究。E-mail：zlw99688@163.com。
通讯作者：刘立科（1974-），男，博士，副教授，从事植物分子遗传学研究。E-mail：liulike@lcu.edu.cn。

更新日期/Last Update: 2013-02-20