DONG Shou-kun,LIU Li-jun,MA Chun-mei,et al.Study on the Role of NH4+-N and NO3--N on Soybean Nutrition Using Labeled 15N[J].Soybean Science,2012,31(06):911-914.[doi:10.3969/j.issn.1000-9841.2012.06.011]
利用15N标记研究铵态氮与硝态氮对大豆的营养作用
- Title:
- Study on the Role of NH4+-N and NO3--N on Soybean Nutrition Using Labeled 15N
- 文章编号:
- 1000-9841（2012）06-0911-04
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 以东农47为材料，采用15N示踪技术，利用组培的方式进行了铵态氮与硝态氮营养作用研究。结果表明，在无菌条件下，以NH4+-N为唯一氮源大豆表现出了铵盐毒害；以NO3--N和混合态氮作为氮源时，大豆可以正常生长。以混合态氮为氮源植株总生物量最大，以NO3--N为氮源次之，以NH+4-N为氮源的处理生物量最小，不同形态氮之间生物量差异达到显著水平。随氮素水平增加，外源氮在植株中比例增加，当培养基中NH+4-N与NO3--N比例为1∶1时，大豆吸收NH+4-N与NO3--N比例为1.5∶1，叶片、茎、根NH+4-N与NO-3-N吸收比例分别为1.6∶1、1.4∶1和1.6∶1，说明硝态氮的存在能够解除铵盐毒害并促进大豆对氮素的吸收利用。
- Abstract:
- This paper studied the role of NH4+-N and NO3--N on soybean(Dongnong47)nutrition using 15N trace technique and tissue culture.The results showed that soybean appeared ammonium salt toxicity when NH4+-N was used as nitrogen source under aseptic condition,soybean could grow normaly when NO3--N and mixed nitrogen (NO3--N∶NH4+-N=1∶1) were used as nitrogen source.The total biomass reached maximum when mixed nitrogen were used as nitrogen source,NO3--N took second place,NH4+-N reached minimum.Biomass differences between the different forms of nitrogen reached a significant level.Exogenous nitrogen absorbed by soybean plants increased with nitrogen level.When the ratio of NH4+-N and NO3--N in culture medium was 1∶1,the ratio of NH4+-N and NO3--N absorbed by soybean was approximately 1.5∶1,and the ratios of NH4+-N and NO3--N absorbed by leaf,stem and root were 1.6∶1,1.4∶1 and 1.6∶1,respectively.Results suggest nitrate nitrogen could eliminate ammonium salt toxicity and promoted nitrogen absorption and utilization in soybean.
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备注/Memo
基金项目：国家“十二五”科技支撑计划项目（2012BAD14B06）；大豆生物学省部共建教育部重点实验室开放基金 (SB11B03)。