GUO Dan-dan,CHEN Hai-feng,YANG Zhong-lu,et al.Analysis of Resistance Inheritance to Soybean Mosaic Virus（SMV）Strain SC-3 and Mapping Related QTL in Soybean[J].Soybean Science,2012,31(04):511-516.[doi:10.3969/j.issn.1000-9841.2012.04.001]
大豆对SMV SC-3株系的抗性遗传和QTL分析
- Title:
- Analysis of Resistance Inheritance to Soybean Mosaic Virus（SMV）Strain SC-3 and Mapping Related QTL in Soybean
- 文章编号:
- 1000-9841（2012）04-0511-06
- Keywords:
- Soybean; Soybean Mosaic Virus; Genetic analysis; QTL
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 为研究大豆对SMV抗扩展的遗传机制，以中豆29×中豆32构建的重组自交系群体（RIL）为材料，人工接种SC-3株系。以病情指数为抗性指标，应用主基因＋多基因混合遗传模型进行遗传分析，利用复合区间作图法进行QTL定位。结果表明:该RIL群体对SC-3株系的抗性遗传符合B-2-3遗传模型，即抗性由2对等加性主基因控制，加性效应为-9.78，主基因遗传率为97.65%。在3个染色体上检测到3个抗性相关QTL，表型贡献率为10.80%～13.41%。
- Abstract:
- In order to study the genetic mechanism of resistance in development to Soybean Mosaic Virus（SMV）in soybean\[Glycine max (L.)Merr.\],a recombinant inbred line(RIL)population consisting of 144 lines derived from Zhongdou No.29 × Zhongdou No.32 was inoculated with the SMV strain SC-3.Genetic analysis was conducted by the major gene plus polygene mixed inheritance model,and QTLs were detected by composite interval mapping(CIM)based on the resistance reaction indicated by disease index.The results showed that the inheritance of resistance to strain SC-3 was controlled by two equality-additive major gene with an additive effect of -9.78 and a heritability of 97.65% for the major genes.Three QTLs were detected on chromosome 6,10 and 13 respectively,explaining 10.80%-13.41% of phenotypic variation.
参考文献/References:
[1]Hill J H.Compenclium of soybean disease,4th edition[M].St.Paul:American Phytopathology Society Press,1999,70-71. [2]智海剑,盖钧镒.大豆对SMV数量抗性的育种[J].大豆科学,2004,23(1):1-5.(Zhi H J,Gai J Y.A study on the utilization of soybean quantitative resistance to Soybean Mosaic Virus[J].Soybean Science,2004,23(1):1-5.) [3]智海剑,盖钧镒.大豆对SMV数量抗性的表现形式与种质鉴定[J].中国农业科学,2004,37(10):1422-1427.(Zhi H J,Gai J Y.Performances and germplasm evaluation of quantitative resistance to Soybean Mosaic Virus in soybeans[J].Scientia Agricultura Sinica,2004,37(10):1422-1427.) [4]盖钧镒,胡蕴珠,崔章林,等.大豆资源对SMV株系的抗性鉴定[J].大豆科学,1989,8(4):323-330.(Gai J Y,Hu Y Z,Cui Z L,et al.An evaluation of resistance of soybean germplasm to Soybean Mosaic Virus[J].Soybean Science,1989,8(4):323-330.) [5]盖钧镒,章元明,王建康.植物数量性状遗传体系[M].北京:科学出版社,2003.(Gai J Y,Zhang Y M,Wang J K.Genetic system of quantitative traits in plants[M].Beijing:Science Press,2003.) [6]智海剑,盖钧镒,何小红.大豆对SMV抗侵染与抗扩展的遗传分析[J].作物学报,2005,31(10):1260-1264.(Zhi H J,Gai J Y,He X H.Inheritance of resistance in infection and resistance in development to Soybean Mosaic Virus in soybeans[J].Acta Agronomica Sinica,2005,31(10):1260-1264.) [7]东方阳.大豆对SMV株系抗性遗传分析和RAPD标记研究[D].南京：南京农业大学,1999.(Dong F Y.Genetic analysis and RAPD markers of genes resistance to SMV strain in the soybean[D].Nanjing:Nanjing Agricultural University,1999.) [8]白丽,李海潮,马莹,等.大豆对大豆花叶病毒SC-11株系抗性的遗传及基因定位[J].大豆科学,2009,28(1):1-4.(Bai L,Li H C,Ma Y,et al.Inheritance and gene mapping of resistance to Soybean Mosaic Virus strain SC-11 in soybean[J].Soybean Science,2009,28(1):1-4.) [9]郭东全,王延伟,智海剑,等.大豆对SMV SC-13株系群的抗性遗传及基因定位的研究[J].大豆科学,2007,26(1):21-23.(Guo D Q,Wang Y W,Zhi H J,et al.Inheritance and gene mapping of resistance to SMV strain group SC-13 in soybean[J].Soybean Science,2007,26(1):21-23.) [10]Fu S X,Zhan Y,Zhi H J,et al.Mapping of SMV resistance gene Rsc-7 by SSR markers in soybean[J].Genetica,2006,128:63-69. [11]Wang D G,Ma Y,Yang Y Q,et al.Fine mapping and analyses of Rsc8 resistance candidate genes to Soybean Mosaic Virus in soybean[J].Theoretical Applied Genetics,2011,122:555-565. [12]程利国.大豆遗传图谱构建和重要性状的QTL定位[D].南京:南京农业大学,2008.(Cheng L G.Construction of genetic linkage map and QTL mapping of important traits in soybean[D].Nanjing:Nanjing Agricultural University,2008.) [13]杨中路.大豆对大豆花叶病毒（SMV）的数量抗性研究及QTL定位[D].南京:南京农业大学,2009.(Yang Z L.Study on quantitative resistance to Soybean Mosaic Virus（SMV）and QTL mapping in soybean[D].Nanjing:Nanjing Agricultural University,2008.) [14]王贤智.大豆产量相关性状的遗传与稳定性分析及QTL定位研究[D].北京:中国农业科学院,2008.(Wang X Z.Inheritance stability analysis and QTL mapping of yield related traits in soybean[D].Beijing:Chinese Academy of Agricultural Science,2008.) [15]Zhi H J,Gai J Y.Performances and germplasm evaluation of quantitative resistance to Soybean Mosaic Virus in soybeans[J].Agricultural Science in China,2004,3(4):247-253. [16]智海剑,盖钧镒,陈应志,等.2002-2004年国家大豆区试品种对大豆花叶病毒抗性的评价[J].大豆科学,2005,24(3):190-193.（Zhi H J,Gai J Y,Chen Y Z,et al.Evaluation of resistance to SMV of the entries in the national uniform soybean tests（2002-2004）[J].Soybean Science,2005,24(3):190-193.) [17]王金社,李海旺,赵团结,等.重组自交系群体4对主基因加多基因混合遗传模型分离分析方法的建立[J].作物学报,2010,36(2):191-201.（Wang J S,Li H W,Zhao T J,et al.Establishment of segregation analysis of mixed inheritance model with four major genes plus polygene in recombinant inbred lines population[J].Acta Agronomica Sinica,2010,36(2):191-201.) [18]章元明,盖钧镒,王永军.利用Pl、P2和DH或RIL群体联合分离分析的拓展[J].遗传,2001,23(5):467-470.（Zhang Y M,Gai J Y,Wang Y J.An expansion of joint segregation analysis of quantitative trait for using P1，P2 and DH or RIL populations[J].Hereditas,2001,23(5):467-470.) [19]周蓉,王贤智,陈海峰,等.大豆倒伏性及其相关性状的QTL分析[J].作物学报,2009,35(1):57-65.（Zhou R,Wang X Z,Chen H F,et al.QTL analysis of lodging and related traits in soybean[J].Acta Agronomica Sinica,2009,35(1):57-65.) [20]周蓉,陈海峰,王贤智,等.大豆幼苗根系性状的QTL分析[J].作物学报,2011,37(7):1151-1158.（Zhou R,Chen H F,Wang X Z,et al.QTL analysis of root traits of soybean at seedling stage[J].Acta Agronomica Sinica,2011,37(7):1151-1158.) [21]王永军.大豆重组自交系群体的构建与调整及其在遗传作图、抗花叶病毒基因定位和农艺及品质性状QTL分析中的应用[D].南京:南京农业大学,2001.(Wang Y J.Establishment and adjustment of RIL population and its application to map construction,mapping resistant to SMV and QTL analysis of agronomic ＆quality traits in soybeans[D].Nanjing:Nanjing Agricultural University,2001.)
相似文献/References:
[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(04):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(04):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(04):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(04):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(04):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(04):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(04):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(04):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(04):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(04):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]栾晓燕,陈怡,杜维广,等.不同抗性大豆品种(系)感染SMV后可溶性糖和游离氨基酸的研究[J].大豆科学,2000,19(04):356.[doi:10.11861/j.issn.1000-9841.2000.04.0356]
[J].Soybean Science,2000,19(04):356.[doi:10.11861/j.issn.1000-9841.2000.04.0356]
[12]方飞,杨云华,王丽群,等.大豆类受体激酶基因GmNIK的克隆与表达分析[J].大豆科学,2019,38(05):704.[doi:10.11861/j.issn.1000-9841.2019.05.0704]
FANG Fei,YANG Yun-hua,WANG Li-qun,et al.Cloning and Analysis of Receptor-like Kinase Gene GmNIK in Soybean[J].Soybean Science,2019,38(04):704.[doi:10.11861/j.issn.1000-9841.2019.05.0704]
备注/Memo
基金项目： 国家自然科学基金（30871554，30900906）；现代农业产业技术体系建设专项资金。