[1]王 伟,王 罡,季 静,等.大豆胚尖再生体系的优化及与子叶节再生体系的比较[J].大豆科学,2012,31(03):353-357.[doi:10.3969/j.issn.1000-9841.2012.03.004]
　WANG Wei,WANG Gang,JI Jing,et al.Optimization of Embryonic Tip Regeneration System and Comparison with Cotyledonary Node Regeneration System in Soybean[J].Soybean Science,2012,31(03):353-357.[doi:10.3969/j.issn.1000-9841.2012.03.004]
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大豆胚尖再生体系的优化及与子叶节再生体系的比较

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第31卷 期数: 2012年03期 页码: 353-357 栏目: 出版日期: 2012-03-25
Title:
Optimization of Embryonic Tip Regeneration System and Comparison with Cotyledonary Node Regeneration System in Soybean
文章编号:
1000-9841（2012）03-0353-05
作者:
王 伟1; 王 罡1; 2; 季 静1; 2; 郑丽红2;  关春峰1;  王 萍3
1.天津大学 农业与生物工程学院，天津 300072；
2.天津大学 化工学院，天津 300072；
3.淮海工学院 海洋学院，江苏 连云港 222005
Author(s):
WANG Wei1; WANG Gang1; 2; JI Jing1; 2; ZHENG Li-hong2; GUAN Chun-feng1; WANG Ping3
1.School of Agriculture and Bioengineering,Tianjin University,Tianjin 300072;
2.School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072;
3.School of Marine Science and Technology,Huaihai Institute of Technology,Lianyungang 222005,Jiangsu,China
关键词:
大豆; 胚尖; 子叶节; 植株再生
Keywords:
Soybean; Embryonic Tip; Cotyledonary node; Plant regeneration
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2012.03.004
文献标志码:
A
摘要:
研究了浸种时间、诱导时间、6-BA浓度3个因素对吉育91胚尖不定芽诱导的影响，并将优化的胚尖再生体系与子叶节再生体系进行比较。结果表明，浸种24 h最适宜胚尖不定芽的形成，不定芽诱导率可达到85.1%，平均芽数可达到4.9个；诱导培养48 h时不定芽诱导率及平均芽数较高，分别为84.2%和5.3个；诱导培养基中6-BA浓度为2.0～3.0 mg·L-1时，不定芽诱导及伸长状况最佳。除了生根率差异不显著外，吉育91胚尖再生体系在再生能力与培养周期上均显著优于子叶节再生体系，因此更适合作为遗传转化的受体材料。
Abstract:
In order to obtain high efficiency soybean regeneration system,the effect of different factors on the shoots induction of embryonic tips was investigated,including soaking time,induction time and 6-BA concentration and compared the optimized embryonic tip regeneration system with cotyledonary node regeneration system.The results indicated that 24 h seed soaking was suitable for adventitious shoots formation,the adventitious shoots induction rate and mean number of shoots could reach 85.1% and 4.9,respectively.The optimum inducing time was 48 h,and the adventitious shoots induction rate and the mean number of shoots were 84.2% and 5.3,respectively.And the optimal concentration of 6-BA in induction medium was 2.0-3.0 mg·L-1.In comparison with cotyledonary node regeneration system,embryonic tip regeneration system was obviously superior in regeneration capacity and culture period，except for rooting rate which had no significant difference.The embryonic tip regeneration system from Jiyu 91 was more suitable for receptor materials in genetic transformation of soybean.

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备注/Memo

基金项目：国家转基因生物新品种培育重大专项（2008ZX08004-001,2009ZX08010-013B，2009ZX08003-019B)。

第一作者简介：王伟（1983-)，女，在读硕士，研究方向为植物转基因。E-mail:wangweisosome@163.com。
通讯作者：王罡 (1964-)，男，教授，博士生导师，主要从事植物基因工程研究。E-mail:wanggangtjdx@126.com。

更新日期/Last Update: 2014-08-16