WANG Juan,LIU Miao,WANG Zhi-kun,et al.Advances in Transgenic Soybean Resistant to Disease and Pest[J].Soybean Science,2011,30(05):865-868,873.[doi:10.11861/j.issn.1000-9841.2011.05.0865]
大豆抗病、虫转基因研究进展
- Title:
- Advances in Transgenic Soybean Resistant to Disease and Pest
- 文章编号:
- 1000-9841(2011)05-0865-04
- Keywords:
- Soybean; Disease and pest resistance; Transgene
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 大豆病、虫害不但造成大豆减产,而且严重影响大豆的品质,随着基因工程研究的深入,利用转基因技术来改良品种的抗性已在许多作物上得到应用。该文对大豆花叶病毒病、大豆霜霉病、大豆蚜虫、大豆根腐病、大豆胞囊线虫和大豆食心虫抗性转基因方面的研究进行了综述,并比较和讨论了各种基因的应用效果及目前抗病、虫转基因研究中存在的问题,对抗病、虫转基因技术的应用前景进行了展望。
- Abstract:
- Disease and pest not only reduce the soybean yield,but also severely affect its quality.This paper reviewed the major genes applied in recent transgenic studies,such as the application of Bt gene in the breeding of soybean with pod borer resistance,protease inhibitor gene in the breeding of resistant soybean for aphid and nematode,and the application of coat protein gene of virus in virus resistant soybean breeding.This study compared the effect of each gene and analyzed the current problems and feasible solutions in transgenic research.At last,the prospect of the transgenic soybean with disease and pest resistance was discussed.
参考文献/References:
[1]郑翠明,常汝镇,邱丽娟.大豆花叶病毒病研究进展[J].植物病理学报,2000,30(2):97-105.(Zheng C M,Chang R Z,Qiu L J.Progress on the disease of soybean mosaic virus[J].Acta Phytopathologica Sinica,2000,30(2):97-105.) [2]唐绂忱.我国首例转基因抗病超高产大豆品种即将投入大田生产[J].新农业,1997(11):16.(Tang B C.The first super high-yielding and disease-resistant transgenic soybean will be produced soon in China[J].New Agriculture,1997(11):16.) [3]Wang X Y,Eggenberger A L,Nutter F W,et al.Pathogen-derived transgenic resistance to soybean mosaic virus in soybean[J].Molecular Breeding,2001,8(2):119-127. [4]徐香玲,李兴华,刘伟华,等.Ri质粒介导大豆花叶病毒外壳蛋白基因转化大豆的研究[J].大豆科学,1996,15(4):279-288.(Xu X L,Li X H,Liu W H,et al.Study on transferring soybeans mosaic vrious coat protein(SMV-CP)gene into soybeans by Ri-plasmid[J].Soybean Science,1996,15(4):279-288.) [5]Furutani N,Hidaka S,Kosaka Y,et al.Coat protein gene-mediated resistance to soybean mosaic virus in transgenic soybean[J].Breeding Science,2006,56(2):119-124. [6]Furutani N,Yamagishi N,Hidaka S,et al.Soybean mosaic virus resistance in transgenic soybean caused by post-transcriptional gene silencing[J].Breeding Science,2007,57(2):123-128. [7]孙文丽.大豆丝氨酸乙醛酸转氨酶基因克隆、序列分析及转基因植株的抗病性研究[D].沈阳:沈阳农业大学,2008:1-40.(Sun L W.Cloning,sequence analysis of the serine glyoxylate aminotransferase gene of soybean,and research on the resistance of transgenic plants[D].Shenyang:Shengyang Agricultural University,2008:1-40. [8]刘德璞,袁鹰,王成武,等.导入外源DNA大豆后代的抗虫性鉴定与筛选[J].大豆科学,2002,21(4):245-249.(Liu D P,Yuan Y,Wang C W,et al.Selecting and evaluation of mutant lines resistant to pest by introducing exogenous DNA into soybean[J].Soybean Science,2002,21(4):245-249.) [9]郭三堆.植物Bt抗虫基因工程研究进展[J].中国农业科学,1995,28(5):8-13.(Guo S D.Engineering of insect-resistant plant with bacillus thuringiensis crystal protein genes[J].Scientia Agricultura Sinica,1995,28(5):8-13.) [10]许文峰,戚正武.蛋白酶抑制剂转基因植物用于农作物抗虫害[J].生命的化学(中国生物化学会通讯),1993(2):20-22.(Xu W F,Qi Z W.Protease inhibitor resistant transgenic plants for crop pests[J].Chemistry of Life(Journal of Chinese Biochemistry Communication),1993(2):20-22.). [11]肖松华,刘剑光,吴巧娟,等.转外源凝集素基因棉花对棉蚜的抗性鉴定[J].棉花学报,2005,17(2):72-78.(Xiao S H,Liu J G,Wu Q J,et al.Identification of Aphis(Aphis gossypii?Glover.)resistant of cotton(Gossypium hirsutum?L.)with trans-exogenous agglutinin gene[J].Acta Gossypii Sinica,2005,17(2):72-78.) [12]高燕会.农杆菌介导的几丁质酶基因及双价抗虫基因对小麦和棉花遗传转化的研究[D].太谷:山西农业大学,2001:15-36.(Gao Y H.Study on Agrobacterium?tumefaciens-mediated chitinase and scorpin venoingene genes transformation of wheat and cotton[D].Shanxi:Shanxi Agricaltural University,2001:15-36.) [13]刘德璞,袁鹰,唐克轩,等.大豆花粉管通道技术转化雪花莲凝集素(GNA)基因[J].分子植物育种,2006,4(5):663-669.(Liu D P,Yuan Y,Tang K X,et al.Transforming the snowdrop lectin(Galanthus nivalis agglutinin,GNA)gene to soybean by pollen tube pathway technique[J].Molecular Plant Breeding,2006,4(5):663-669.) [14]马晓红.大豆高效整个子叶节再生体系的建立及根癌农杆菌介导的双价抗虫基因在大豆上的转化[D].上海:上海交通大学,2008:17-46.(Ma X H.Efficient regeneration in soybean[Glycine max(L.)Merrill] from whole cotyledonary node explants and Agrobacterium-mediated transformation in soybean for expression of binary insect resistance genes[D].Shanghai:Shanghai Jiaotong University,2008:17-46.) [15]辛惠普,马汇泉,刘静茹,等.大豆根腐病发生与防治的初步研究[J].大豆科学,1987,6(3):189-196.(Xin H P,Ma H Q,Liu J R,et al.A preliminary study on epidemiology and control of disease[J].Soybean Science,1987,6(3):189-196.) [16]徐香玲,邹联沛,刘伟华,等.向大豆导入几丁质酶基因的初步研究[J].大豆科学,1999,18(2):101-108.(Xu X L,Zou L P,Liu W H,et al.A preliminary study on transferring chitinase gene into soybeans[J].Soybean Science,1999,18(2):101-108.) [17]Salehi A,Mohammadi M,Okhovvat S M,et al.Chitinase gene transformation through Agrobacterium?and its explanation in soybean in order to induce resistance to root rot caused by Rhizoctonia solani[J].Communications in Agricultural and Applied Biological Sciences,2005,70(3):399-406. [18]李建国.核糖体失活蛋白的研究进展[J].分子植物育种,2005,3(4):566-570.(Li J G.The study progress on ribosome-inactivating proteins[J].Molecular Plant Breeding,2005,3(4):566-570.) [19]Li H Y,Zhu Y M,Chen Q,et al.Production of transgenic soybean plants with two anti-fungal protein genes via Agrobacterium?and particle bombardment[J].Biologia Plantarum,2004,48(3):367-374. [20]郭玉双,张艳菊,朱延明,等.转几丁质酶和核糖体失活蛋白双价基因大豆的获得与抗病性鉴定[J].作物学报,2006,32(12):1841-1847.(Guo Y S,Zhang Y J,Zhu Y M,et al.Obtainment of transgenic soybean plants with chitinase and ribosome inactivating protein genes and their resistance identification[J].Acta Agronomica Sinica,2006,32(12):1841-1847.) [21]田中艳.利用外源DNA直接导入方法进行大豆抗线育种研究[J].黑龙江农业科学,2003(5):27-29.(Tian Z Y.Study on the breeding of cyst nematode resistance by introducing of foreign DNA into soybean[J].Heilongjiang Agricultural Science,2003(5):27-29.) [22]Ornatowski W,Jayaraj J,Todd T C,et al.Introduction and constitutive expression of a tobacco hornworm(Manduca sexta)chitinase gene in soybean[J].In Vitro Cellular & Developmental Biology-Plant,2004,40(3):260-265. [23]Steeves R M,Todd T C,Essig J S,et al.Transgenic soybeans expressing siRNAs specific to a major sperm protein gene suppress Heterodera glycinesreproduction[J].Functional Plant Biology,2006,33(11):991-999. [24]Marra B M,Souza D S L,Aguiar J N,et al.Protective effects of a cysteine proteinase propeptide expressed in transgenic soybean roots[J].Peptides,2009,30(5):825-831. [25]王丕武,武丽敏,张君,等.Bt+CpTI抗虫基因转化大豆的研究[C].海南:第四届全国植物分子育种学术研讨会,2004.(Wang P W,Wu L M,Zhang J,et al.A study on transformation of Bt+CpTI?insect resistant gene into soybean[C].Hainan:The fourth National Symposium on Plant Molecular Breeding,2004.) [26]郭东全,杨向东,包绍君,等.转CryIA和CpTI双价抗虫基因大豆的获得与稳定表达[J].中国农业科学,2008,41(10):2957-2962.(Guo D Q,Yang X D,Bao S J,et al.Synchronous expression of CryIA?and?CpTI?genes in soybean and analysis of their resistance to insect pests[J].Scientia Agricultura Sinica,2008,41(10):2957-2962.) [27]武小霞,李静,王志坤,等.cry1Ia1基因转化大豆及抗虫性的初步评价[J].上海交通大学学报(农业科学版),2010,28(5):413-419.(Wu X X,Li J,Wang Z K,et al.Transformation of?cry1Ia1 into soybean and rough assessing its resistance to soybean pests[J].Journal of Shanghai Jiaotong University(Agricultural Science),2010,28(5):413-419.)
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备注/Memo
基金项目:国家转基因作物新品种培育重大专项资助项目(2008ZX08004-002)。