WANG Wei-qi,HOU Wen-sheng.Clone and Analysis of Thioredoxin Gene from Soybean (Glycine max L.)[J].Soybean Science,2011,30(03):351-355.[doi:10.11861/j.issn.1000-9841.2011.03.0351]
大豆硫氧还蛋白基因的克隆与分析
- Title:
- Clone and Analysis of Thioredoxin Gene from Soybean (Glycine max L.)
- 文章编号:
- 1000-9841(2011)03-0351-05
- Keywords:
- Soybean; Salt; Thioredoxin gene; Gene clone
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 通过对大豆耐盐品种文丰7盐处理抑制差减文库的筛选，获得了一些差异表达的EST序列，与NCBI中EST数据库进行比对分析后发现，其中1个与硫氧还蛋白相关。据此预测了大豆硫氧还蛋白(Thioredoxin，Trx)基因的cDNA序列，并采用RT-PCR方法克隆了大豆Trx基因。生物信息学分析表明：该基因包含1个354 bp的完整开放阅读框，编码118个氨基酸，与拟南芥和烟草的同源性分别为72%和76%。Real-time PCR结果显示：盐处理后，Trx基因在耐盐和盐敏感品种中的表达量均有所提高，但耐盐品种的上调幅度显著高于盐敏感品种，说明其可能具有提高大豆品种耐盐性的作用。
- Abstract:
- Based on screening the suppression subtractive hybridization library which constructed by soybean cultivar ‘wenfeng7’ (salt-tolerant) after salt treatment, we obtained some differentially expressed EST sequences. The EST sequences were compared with the NCBI databases, analysis result showed that one EST sequence related to thioredoxin. A novel thioredoxin gene (Trx) of Glycine max was cloned and identified with the EST obtained from suppression subtractive hybridization library. Bioinformatics analysis showed that the full length of the GmTrx gene cDNA was 354 bp, coded 118 amino acids. Compared with several plants, this gene showed 72%and 76%homology to thioredoxin protein in Arabidopsisand tobacco, respectively. The expression of different soybean varieties under salt and common treatment was analyzed by Real-time PCR method. The result showed that, the expression of Trx gene was up-regulated in both salt-tolerant and sensitive soybean cultivars after salt treatment, although the expression in salt-tolerant cultivars was much higher than that in salt-sensitive cultivars. This gene may be very important for improving the salt tolerance of soybean.
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备注/Memo
基金项目：国家自然科学基金资助项目（30771358）。