CHEN Zhen-wu,QIAN Lang,WANG Hai-ying,et al.Effect of Nitrogen Fertilizer on Leaf Physiological and Biochemical Traits of Black Soybean during Reproductive Stage[J].Soybean Science,2010,29(04):623-626.[doi:10.11861/j.issn.1000-9841.2010.04.0623]
花期追施氮肥对不同株型黑豆品种生殖生长期叶片生理生化特性的影响
- Title:
- Effect of Nitrogen Fertilizer on Leaf Physiological and Biochemical Traits of Black Soybean during Reproductive Stage
- 文章编号:
- 1000-9841（2010）04-0623-04
- Keywords:
- Black soybean; Plant type; Nitrogen fertilizer; Photosynthesis characteristic; Protective enzymes activities
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 选用沈黑3号（直立型）和辽黑1号（半蔓生型）2个黑豆品种为材料，设置0 kg·hm-2 (N0)、45 kg·hm-2 (N1)、90 kg·hm-2(N2)和90 kg·hm-2+叶面喷施22.5 kg·hm-2(N3)4个追施氮肥处理，探讨了花期追施施氮对不同株型黑豆品种叶片生理生化特性的影响。结果表明：不同施氮处理并没有显著改变黑豆叶片不同生育期叶绿素含量、净光合速率、蒸腾速率、气孔导度和胞间CO2浓度等的变化规律，辽黑1号的各光合生理指标明显高于沈黑3号。不同施氮处理间的叶绿素含量和净光合速率呈现明显的差异，而叶片蒸腾速率、气孔导度和胞间CO2浓度差异不显著。在开花期、结荚期和鼓粒期，随着施氮量的增加，2个黑豆品种的SOD、POD和CAT活性均逐渐增强；辽黑1号叶片的SOD、POD和CAT活性均高于沈黑3号。不同施氮处理相比较，N2对生理指标影响大于N3，而N3对生化指标影响大于N2；品种间比较，辽黑1号优于沈黑3号。
- Abstract:
- Erect black soybean variety of Shenhei No.3 and semi-rampancy variety of Liaohei No.1 were used as materials.Four nitrogen fertilizer application levels,0 kg·hm-2(N0),45 kg·hm-2(N1),90 kg·hm-2(N2) and 90 kg·hm-2 plus leaf spraying 22.5 kg·hm-2(N3),were set.Photosynthetic traits of chlorophyll content(CC),net photosynthetic rate(Pn),transpiration rate(Tr),stomatal conductance (Cond) and intercellular carbondioxide concentration (Ci) as well as protective enzymes of superoxide dismutase(SOD),peroxidase (POD) and catalase (CAT) at reproductive stage were determined.The purpose of this study was to discuss the effects of different nitrogen fertilizer levels on physiological and biochemical characteristics in different black soybean varieties.Nitrogen treatment didn’t change the changing trend of the tested photosynthetic indexes,the CC，Pn，Tr，Cond and Ci of Liaohei No.1 were significantly higher than those of Shenhei No.3.CC and Pn varied significantly between nitrogen treatments,while no distinctive difference were found for Tr，Cond and Ci.With the increment of nitrogen amount,SOD,POD and CAT of both cultivar showed increasing trend.SOD,POD and CAT activity of Liaohei No.1 were higher than those of Shenhei No.3.In conclusion,N3 had more influences on photosynthetic traits,while N2 shows more effects on protective enzymes.Besides,Liaohei No.1 performed better than Shenhei No.3.
参考文献/References:
[1]洪池.中华名贵豆-药黑豆[J].农村科技开发，1995，25（4）：31.（Hong C.China rare bean and drug-black bean[J].Rural Technology Development,1995,25(4):31.） [2]覃初贤.桂西黑大豆资源主要性状观察和品质分析〔J〕.广西农业科学，1995（4）：158-160.（Tan C X.Main character observation and quality analysis of black soybean resources in Guixi[J].Guangxi Agricultural Sciences,1995(4):158-160.） [3]王丹英，汪自强.播期、密度、氮肥用量对菜用大豆产量和品质的效应[J].浙江大学学报(农业与生命科学版)，2001，27（1）：69-72.（Wang D Y，Wang Z Q.Effect of planting date，plant density and nitrogen application rate on yield and quality of vegetable soybean[J].Journal of Zhejiang University(Agriculture.＆ Life Science)，2001,27(1):69-72.） [4]刘丽君，孙聪姝，刘艳，等.氮肥对大豆结瘤及叶片氮素积累的影响〔J〕.东北农业大学学报，2005，36（2）：133-137.（Liu L J，Sun C S，Liu Y，et al.Effects of nitrogen on nodule-forming and nitrogen concentration in soybean leaves[J].Journal of Northeast Agricultural University,2005,36(2):133-137.） [5]张瑞朋,佟斌，傅连舜，等.氮肥对不同来源大豆品种叶片保护酶的影响[J].大豆科学，2009，28（5）：833-836.( Zhang R P,Tong B,Fu L S，et al.Effects of top-dressing nitrogen on protective enzymes of soybean leaves from different regions[J].Soybean Science,2009,28(5):833-836.) [6]张建新，倪丽，翟云龙.施氮对高产春大豆氮素吸收分配的影响[J].大豆科学，2005，24（1）：38-42.（Zhang J X,Ni L,Zhai Y L.Effect on nitrogen fertilizer application to the absorption and distribution of nitrogen in spring soybean[J].Soybean Science,2005,24(1):38-42.） [7]黄正来，武立权，韩立德.花期追施氮肥对菜用大豆AC10生理指标及产量影响的研究[J].激光生物学报，2005，14（3）：193-196.（Huang Z L，Wu L Q，Han L D.Effect of dressing nitrogen on physiological index and yield vegetable of soybean variety AC10 at anthesis[J].Acta Laser Biology Sinica,2005,14(3):193-196.） [8]邹琦.植物生理实验指导[M].北京：中国农业出版社，2000:208-211.（Zou Q.Plant physiology experimental guide [M].Beijing:China Agriculture Press,2000:208-211.） [9]张宪政.作物生理研究法[M].北京：农业出版社，1992:217-220.（Zhang X Z.Crop physiology research methods [M].Beijing:Agricultural Publishing House,1992:217-220.）
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备注/Memo
基金项目：辽宁省科技厅资助项目（2006201008）。