WANG Shu-ming,WANG Yue-qiang,LI Jian-ping,et al.Seed Production of Soybean Cytoplasmic Male Sterile (CMS) Line under Field Conditions[J].Soybean Science,2010,29(03):385-389.[doi:10.11861/j.issn.1000-9841.2010.03.0385]
田间开放条件下大豆不育系制种技术研究
- Title:
- Seed Production of Soybean Cytoplasmic Male Sterile (CMS) Line under Field Conditions
- 文章编号:
- 1000-9841(2010)03-0385-05
- Keywords:
- Soybean; CMS lines; Cross-pollination; Alfalfa leafcutting bees; Seed setting
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 以细胞质雄性不育系JLCMS82A和其保持系JLCMS82B为试材,采用裂区试验设计,在田间开放条件下对不同苜蓿切叶蜂释放量和父母本种植方式下的制种效果进行了研究。结果表明:不同放蜂量处理之间不育系结实率和不育系与保持系总产量的差异达极显著水平,不育系间的产量差异达显著水平;保持系与不育系不同行比处理间的结实率以及不育系与保持系总产量的差异达极显著水平,而不育系间的产量差异不显著;放蜂量与行比间的互作差异不显著。不同放蜂量下,每公顷放3万头蜂时不育系的结实率、产量及总产量达最高;不同行比下,不育系间的产量差异并不显著,而父母本1∶1时不育系的结实率和总产量最高。综合评价,在每公顷释放3万头苜蓿切叶蜂并以父母本1∶1行比种植时,不育系的结实率、产量以及不育系加保持系总产量均达最高,制种效果最佳。
- Abstract:
- The line JLCMS82A and its maintainer line JLCMS82B were used in this trial with a split block design for evaluating the effects of cross-pollination of alfalfa leafcutting bees in different treatments of bee amount and row cytoplasmic male sterile (CMS) ratio of male and female parents. The results showed that the seed setting of the CMS line and the whole yield of the male and female parents existed significant difference at the 1% level and the yield of the CMS line at the 5% level among the different treatments of bee amount. There was significant difference at the 1% level in the seed setting of the CMS line and the whole yield of the male and female parents, whereas the difference of the yield of the CMS line was not significant among the different treatments of row ratio of male and female parents. There was no significant difference between the treatments of bee amount and row ratio of male and female parents. In the treatment of different bee amount, the highest seed setting and yield of the CMS line and the whole yield of the male and female parents were observed when the amount of alfalfa leafcutting bees reached 30,000/ha. In the treatment of different row ratio of male and female parents, the highest seed setting and whole yield of the male and female parents were also observed when the row ratio was 1∶1, whereas there was no significant difference for the yield of the CMS line between the different row ratio. On the whole, the best effective treatment was that of the 1∶1row ratio with the amount of 30,000 alfalfa leafcutting bees per hectare.
参考文献/References:
[1]Sun H, Zhao L M, Huang M. Studies on cytoplasmic-nuclear male sterile soybean[J].Chinese Science Bulletin, 1994, 39(2): 175-176. [2]Gai J Y, Cui Z L, Ji D F. A report on the nuclear cytoplasmic male sterility from a cross between two soybean cultivars[J].Soybean Genetics Newsletter, 1995, 22: 55-58. [3]张磊, 戴瓯和. 大豆质核互作不育系W931A的选育研究[J].中国农业科学, 1997, 30(6): 90-91.(Zhang L,Dai O H. Selection and breeding of nucleo- cytoplasmic male sterile line W931A in soybean [J].Scientia Agricultura Sinica, 1997, 30(6): 90-91.) [4]赵丽梅, 孙寰, 黄梅. 大豆细胞质雄性不育系ZA的选育和初步研究[J].大豆科学, 1998, 17(3): 268-270.( Zhao L M, Sun H, Huang M. The development and preliminary studies on cytoplasmic male sterile soybean line ZA[J].Soybean Science, 1998, 17(3): 268-270.) [5]张磊, 戴瓯和, 张亚丽. 大豆质核互作雄性不育系W945A、W948A的选育[J].大豆科学, 1999,18(4): 327-330.( Zhang L,Dai O H,Zhang Y L. Breeding of soybean male sterile line of nucleo- cytoplasmic interaction[J].Soybean Science, 1999,18(4): 327-330.) [6]李智. 阜阳三系杂交大豆研究进展[J].作物杂志, 2007(5): 87-89.(Li Z. Research progress of hybrid soybean in Fuyang[J].Crops, 2007(5): 87-89.) [7]Boerma H R, Moradshahi A. Pollen movement within and between rows to male-sterile soybeans[J]. Crop Science, 1975, 15: 858-861. [8]May M L, Wilcox J R. Pollinator density effects on frequency and randomness of male-sterile soybean pollinations[J]. Crop Science,1986, 26: 96-99. [9]Koelling P D, Kenworthy W J, Caron D M. Pollination of male-sterile soybeans in caged plots[J]. Crop Science,1981, 21: 559-561. [10]Roumet P, Magnier I. Estimation of hybrid seed production and efficient pollen flow using insect pollination of male sterile soybeans in caged plots[J]. Euphytica, 1993, 70:61-67. [11]赵丽梅, 孙寰, 马春森, 等. 大豆昆虫传粉研究初探[J].大豆科学, 1999,18(1): 73-76.( Zhao L M, Sun H,Ma C S, et al. Preliminary study of soybean pollination by bees[J].Soybean Science, 1999,18(1): 73-76.) [12]丁德荣, 盖钧镒. 南方地区大豆雄性不育材料的传粉昆虫媒介及其传粉异交结实程度[J].大豆科学, 2000,19(1): 74-79.(Ding D R, Gai J Y. Pollinating insects and natural outcrossing amount of soybean male-sterile lines [J]. Soybean Science, 2000,19(1): 74-79.) [13]于伟, 李磊, 李智, 等. 大豆质核互作不育系杂交制种技术研究[J].中国油料作物学报, 2001,23(2):11-13.(Yu W,Li L,Li Z, et al. Studies on hybrid seed production of cytoplasmic male sterile lines in soybean[J].Chinese Journal of Oil Crop Sciences, 2001,23(2):11-13.) [14]李建平, 李茂海, 杨桂华, 等. 大豆不育系传粉昆虫及传粉技术研究[J].吉林农业科学, 2002, 27(增):4-6.(Li J P,Li M H,Yang G H, et al. Study of pollinating insects and pollinating technique of soybean male sterile plants[J].Journal of Jiln Agricultural Sciences, 2002, 27(Supplement): 4-6.) [15]杨桂华,李建平,李茂海,等. 熊蜂和苜蓿切叶蜂在网室内对大豆不育系授粉效果的研究[J].吉林农业科学,2005,30(3):21-22,28. (Yang G H, Li J P, Li, et al. Studies on pollinating soybean male sterile plant in caged plots using bumble bee and alfalfa leaf-cutting bee[J].Journal of Jiln Agricultural Sciences, 2005, 30(3): 21-22,28.) [16]孙寰, 赵丽梅, 王曙明, 等. 大豆杂种优势利用研究进展[J].中国油料作物学报, 2003,25(1):92-96.( Sun H, Zhao L M, Wang S M. et al. A review on research progress of soybean heterosis utilization[J].Chinese Journal of Oil Crop Sciences, 2003,25(1):92-96.)
相似文献/References:
[1]刘章雄,李卫东,孙石,等.1983~2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(03):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(03):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(03):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(03):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(03):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(03):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(03):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(03):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(03):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(03):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]费晓艳,赵洪锟,刘晓东,等.大豆细胞质雄性不育系配套保持系线粒体基因组BAC文库的构建[J].大豆科学,2011,30(03):401.[doi:10.11861/j.issn.1000-9841.2011.03.0401]
FEI Xiao-yan,ZHAO Hong-kun,LIU Xiao-dong,et al.BAC Library Construction for Mitochondrial Genome of A Maintainer(JLCMS1) Associated with Cytoplasmic Male Sterility Soybean[J].Soybean Science,2011,30(03):401.[doi:10.11861/j.issn.1000-9841.2011.03.0401]
[12]张伟龙,张伟,徐长洪,等.父母本不同行距配置对昆虫授粉效率的影响[J].大豆科学,2017,36(05):733.[doi:10.11861/j.issn.1000-9841.2017.05.0733]
ZHANG Wei-long,ZHANG Wei,XU Chang-hong,et al.Effect of Different Parents Row Spacing on the Efficiency of Insect Pollination[J].Soybean Science,2017,36(03):733.[doi:10.11861/j.issn.1000-9841.2017.05.0733]
备注/Memo
基金项目:国家高技术研究发展计划资助项目(2002AA207007);吉林省科技发展计划资助项目(20010211)。