WANG Zhen-dong,CHEN Chao-li,YU Bai-shuang,et al.SSR Analysis of Genetic Diversity of Soybean Germplasm in Drought Resistance[J].Soybean Science,2010,29(03):370-373.[doi:10.11861/j.issn.1000-9841.2010.03.0370]
大豆抗旱种质资源遗传多样性的SSR分析
- Title:
- SSR Analysis of Genetic Diversity of Soybean Germplasm in Drought Resistance
- 文章编号:
- 1000-9841（2010）03-0370-04
- Keywords:
- Soybean; Genetic diversity; SSR; Cluster analysis
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 以45份具有不同程度抗旱性的大豆为材料，利用SSR分子标记对这些材料进行分析，选取2个产地、农艺性状差异显著的大豆品种静乐黑滚豆和辽13，对SSR引物进行筛选，从中选择出多态性丰富，带型较好的引物11对。用筛选出的引物对45份大豆种质进行PCR扩增。聚类结果表明：GD=0.41处可将45个大豆品种分成两大类，大部分品种被归为第1类，有31个品种，占总品种数的69%，第2类包含14品种，占总品种数的31%。两个大类又各自细分成不同的亚类群，聚类结果反映出品种间关系与地理起源、表型形态等具有一定的相关性。
- Abstract:
- The genetic diversity of 45 soybean germplasms including different degrees of drought resistance were analyzed by SSR method. Eleven pair of primers with rich polymorphism and good model were selected from ‘Jingle black’ and ‘Liao 13’, of which the place of origin and the agronomic traits are farther apart. The 11pair of primers were used to amplify the genome DNA of 45 materials. Clustering results showed that 45 soybean varieties could be divided into two major categories while GD=0.41, most of the varieties were classified as first category, there are 31accessions, accounting for 69% of the total;the second category consisted of 14 accessions, accounted for 31% of the total. Two major categories were subdivided into their different sub-group, The cluster results exhibited their relationship related with the geographic origin and appearance.
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备注/Memo
基金项目：辽宁省博士启动基金资助项目（20071060）。