[1]陈远东,喻德跃.EMS诱发大豆“南农94-16”突变体库的扩建及部分突变体的SSR分析[J].大豆科学,2009,28(04):574-577,582.[doi:10.11861/j.issn.1000-9841.2009.04.0574]
　CHEN Yuan-dong,YU De-yue.Construction of Mutant Pools for Soybean “Nannong 94-16” Induced by EMS and Analysis of SSR Marker on Several Mutants[J].Soybean Science,2009,28(04):574-577,582.[doi:10.11861/j.issn.1000-9841.2009.04.0574]
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EMS诱发大豆“南农94-16”突变体库的扩建及部分突变体的SSR分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年04期 页码: 574-577,582 栏目: 出版日期: 2009-08-25
Title:
Construction of Mutant Pools for Soybean “Nannong 94-16” Induced by EMS and Analysis of SSR Marker on Several Mutants
文章编号:
1000-9841(2009)04-0574-04
作者:
陈远东; 喻德跃
南京农业大学大豆研究所 国家大豆改良中心 作物遗传与种质创新国家重点实验室，江苏 南京 210095
Author(s):
CHEN Yuan-dong; YU De-yue
Soybean Research Institute，Nanjing Agricultural University，National Center for Soybean Improvement,State Key Laboratory of Crop Genetics and Germplasm Enhancement，Nanjing 210095，Jiangsu，China
关键词:
EMS; 大豆; 突变体; SSR
Keywords:
EMS; Mutants; SSR; Soybean
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.04.0574
文献标志码:
A
摘要:
甲基磺酸乙酯(EMS)是一种稳定、高效的诱发点突变的诱变剂。采用EMS处理大豆“南农94-16”的种子，在初步构建大豆突变体库的基础上进一步扩建了大豆突变体库，获得95份茎、叶、花、种子等性状变异的突变材料。采用200对SSR 标记分别对其中高蛋白、叶黄化等9个不同性状的突变体进行了鉴定。结果表明：与对照相比突变体发生了多位点的变异。构建的突变体库不但可以作为新的种质资源，而且可促进大豆功能基因组研究的开展。
Abstract:
EMS is a stable and effective mutagen.In this study，soybean mutant pools were constructed by treating the seeds of soybean“Nannong 94-16”with EMS.These pools include 95 mutants of stem，leaf，cotyledon，flower and seed.In addition，200 SSR primer pairs were used to characterize several mutants such as high-protein content mutant，revealing polymorphism between mutants and base materials.These mutant pools created for“Nannong 94-16”will be valuable for the research in breeding and functional genomics soybean.

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备注/Memo

基金项目：国家重点基础研究发展计划（973计划）资助项目（2004CB117206）；国家高技术研究发展计划（863计划）资助项目（2006AA10Z1C1）。

作者简介：陈远东 (1983-),男,硕士研究生,研究方向为植物基因工程。E-mail:chenyd0926@163.com。
通讯作者：喻德跃,教授,博士生导师。E-mail:dyyu @njau.edu.cn。

更新日期/Last Update: 2014-09-21