[1]刘金波,许艳丽,吕国忠,等.黑土区不同轮作系统大豆根际镰孢菌种群结构和数量[J].大豆科学,2009,28(01):97-102.[doi:10.11861/j.issn.1000-9841.2009.01.0097]
　LIU Jin-bo,XU Yan-li,L Guo-zhong,et al.Black Soil Region Fusarium Population Structure and Quantity in Soybean Rhizosphere of Different Rotation System[J].Soybean Science,2009,28(01):97-102.[doi:10.11861/j.issn.1000-9841.2009.01.0097]
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黑土区不同轮作系统大豆根际镰孢菌种群结构和数量

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年01期 页码: 97-102 栏目: 出版日期: 2009-02-25
Title:
Black Soil Region Fusarium Population Structure and Quantity in Soybean Rhizosphere of Different Rotation System
文章编号:
1000-9841(2009)01-0097-06
作者:
刘金波1; 2; 许艳丽1; 吕国忠3; 李春杰1; 赵志慧4; 魏巍5
1中国科学院东北地理与农业生态研究所，黑龙江 哈尔滨 150081；
2中国科学院研究生院，北京 100049；
3大连民族学院生物资源与环境研究所，辽宁 大连 116600；
4沈阳农业大学植物保护学院，辽宁 沈阳 110161；
5东北林业大学生命科学学院，黑龙江 哈尔滨 150040
Author(s):
LIU Jin-bo1; 2; XU Yan-li1; L Guo-zhong3; LI Chun-jie1; ZHAO Zhi-hui4; WEI Wei5
1 Northeast Institute of Geography and Agro ecology，CAS，Harbin 150081，Heilongjiang；
2Graduate School，CAS，Beijing 100049；
3Institute of Bio- Resources and Environment，Dalian Nationalities University，Dalian 116600，Liaoning；
4College of Plant Protection，Shenyang Agricultural University，Shenyang 110161，Liaoning；
5 College of life science，Northesat Forestry University，Harbin 150040，Heilongjiang，China
关键词:
大豆; 根际土壤; 镰孢菌; 形态学鉴定
Keywords:
Soybean; Rhizosphere soil; Fusarium; Morphological identification
分类号:
S154.37
DOI:
10.11861/j.issn.1000-9841.2009.01.0097
文献标志码:
A
摘要:
对中国科学院海伦农业生态试验站长期定位区大豆连作17年、小麦-玉米-大豆轮作、玉米-大豆迎茬和大豆连作2年，分别取分枝期、花期和鼓粒期的大豆根际土壤，采用土粒平板法进行镰孢菌分离，探讨大豆连作对镰孢菌种群结构和数量的影响。根据形态学特征鉴定出6种镰孢菌，即锐顶镰孢（F.acuminatum Ellis & Everhart）、黄色镰孢（F.culmorum（W.G.Smith）Sacc.）、木贼镰孢（F.equiseti (Corda）Sacc.）、禾谷镰孢（F.graminearum Schwabe）、尖孢镰孢（F.oxysporum Schlecht.）和腐皮镰孢（F.solani(Mart.）Sacc.）。在不同生育期及不同轮作系统大豆根际土壤中尖孢镰孢（F.oxysporum）数量较高，与其它种类相比差异显著(P<0.05)，是大豆根际土壤中的优势菌。不同轮作系统大豆根际土壤以尖孢镰孢数量平均值最高，其次为腐皮镰孢；不同轮作系统镰孢菌总量动态变化一致，花期轮作镰孢菌数量最高为1.54×104个g-1干土，与大豆连作17年相比差异明显。在3个生育期大豆连作17年尖孢镰孢数量均略低于轮作。
Abstract:
Fusarium is one of the main pathogen of soybean root rot，distribution of Fusarium in soil is surprisingly diverse，its mycelium and chlamydospore can be survival many years and difficult to prevent.Isolates of Fusarium were obtained by direct soil plating method with soils from soybean monoculture 17 years（S -S-S)，Wheat-Corn-Soybean rotation（W-C-S W-C-S)，Corn-Soybean rotation(C-S C-S）and soybean monoculture 2 years（C-S-S C-S-S) in Hailun Agricultural Ecology Experimental Station of CAS，and rhizosphere soils were sampled at branching，flowering and seed-filling stage，the research was carried to probe the effect of soybean monoculture on Fusarium population structure and quantity.Based on morphological characteristics，these isolates were identified to 6 species，namely Fusarium acuminatum Ellis &EverhartF.culmorum（W.G.Smith)Sacc，F.equiseti（Corda）Sacc，F.oxysporum Schlecht and F.solani（Mart.）Sacc.The quantity ofF.oxysporum in soybean rhizosphere soil in different growth stages and different rotation systems was significantly(P<0.05)higher compared to other Fusarium species，indicating F.oxysporum was the dominant specie of soybean rhizosphere soil.The average quantity ofF.oxysporum was the highest in different rotation systems soybean rhizosphere soil，then was F.solani.The Fusarium spp.total quantity dynamics were the same in different rotation systems.In florescence stage，the Fusarium spp.total quantity of W-C-S W-C-S which was 1.54×104?CFU per gram dry soil was the highest and significant different to S -SS.The quantity of F.oxysporum in S -S-S were lower than W-C-S W-C-S in three soybean growth stages.

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备注/Memo

基金项目：中国科学院知识创新工程重要方向资助项目（kzcx2-yw-408）;国家“十一五”科技支撑计划资助项目（GA06B101）;中国科学院东北地理与农业生态研究所黑土生态重点实验室基金课题（HTST07）资助项目。

作者简介：刘金波（1982-），女，在读硕士，现主要从事土壤微生物生态研究。

更新日期/Last Update: 2014-10-01