[1]王振东,贾利,孙仓,等.大豆抗旱种质资源遗传多样性的RAPD分析[J].大豆科学,2009,28(01):28-30,35.[doi:10.11861/j.issn.1000-9841.2009.01.0028]
　WANG Zhen-dong,JIA Li,SUN Cang,et al.RAPD Analysis of Genetic Diversity of Soybean Germplasm in Drought Resistance[J].Soybean Science,2009,28(01):28-30,35.[doi:10.11861/j.issn.1000-9841.2009.01.0028]
点击复制

大豆抗旱种质资源遗传多样性的RAPD分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年01期 页码: 28-30,35 栏目: 出版日期: 2009-02-25
Title:
RAPD Analysis of Genetic Diversity of Soybean Germplasm in Drought Resistance
文章编号:
1000-9841(2009)01-0026-05
作者:
王振东1; 贾利1; 孙仓1; 田秀艳2; 王惠1
1 沈阳农业大学生物科学技术学院，辽宁 沈阳 110161；
2 沈阳市农业检测中心，辽宁 沈阳 110034
Author(s):
WANG Zhen-dong1; JIA Li1; SUN Cang1; TIAN Xiu-yan2; WANG Hui1
1Science and Technology Department of Shenyang Agricultural University，Shenyang 110161，Liaoning；
2Shenyang Agricultural Examination Center，Shenyang 110034，Liaoning，China
关键词:
大豆; 遗传多样性; RAPD; 聚类分析
Keywords:
Soybean; Genetic diversity; RAPD; Cluster analysis
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.01.0028
文献标志码:
A
摘要:
采用RAPD技术对45个不同抗旱程度的大豆种质进行遗传多样性分析。从115条随机引物筛选出10条重复性好、扩增条带清晰的引物，然后对45个品种基因组DNA进行扩增，结果共扩增出86条片段，其中同源片段17条，占带总数的19.8%，多态性片段69条，占片段总数的80.2%，平均每条引物扩增出多态性片段6.9条，大部分片段介于200~2000 bp之间。利用NTSYS-Pc软件计算品种间遗传相似系数（GS）和遗传距离（GD），45个品种之间的遗传相似系数介于0.3818~0.9038之间。在GD =0.38处，将45份抗旱性不同的大豆品种分成3大类，第一类有40个品种，占品种总数的90%，第二类包含2个品种，分别是低抗旱的6178和不抗旱的大白眉，第三类有3个品种，分别是不抗旱的Fayette、牛毛黄和90-1105。在GD=0.29处，第一大类群又按照抗旱性的高低分别聚成高抗旱、中抗旱、低抗旱、不抗旱等10个亚类群。聚类结果反映出品种间关系与地理起源、表型形态等具有一定的相关性。
Abstract:
The genetic diversity of 45 soybean germplasms including different degrees of drought resistance were analyzed by RAPD method.Among 115 RAPD primers，10 repeatitive RAPD primers were selected to amplify the genome DNA of 45 materials.A total of 86 fragments were amplified by RAPD.There were 17 homologous fragments among all the clear and stable amplified fragments，account for 19.8 % of the total amplified fragments.In average，the amplified fragment polymorphism was 6.9 and most of the amplified fragments ranged from 200bp to 2 000 bp.The genetic similarity coefficient and genetic distance among forty-five species were calculated by using NTSYS-Pc and the coefficient ranged from 0.3818 to 0.9038.When the genetic distance was at 0.38，forty-five species were divided into three groups，there were forty species in the first group，accounting for 90%.There were two species which were 6 178 and Dabaimei in the second group，whereas the third group consisted of three species，which were Fayette，Niumaohuang and 90-1105.When the genetic distance was at 0.29，the first group could be divided into ten sub-groups.The cluster results exhibited their relationship related with the geographic origin and appearance.

参考文献/References:

[1]张福锁.环境胁迫与植物育种[M].北京：农业出版社，1993：120-123.(Zhang F S.Environmental stress and plant breeding[M].Beijing：Agricultural Press，1993：120-123.)

[2]山仑，陈国良.黄土高原旱地农业的理论与实践[M].北京：科学出版社，1993：125-129.（Shan L，Chen G L.Theory and practice of dryland agriculture in the loess plateau[M].Beijing：Science Press，1993：125-129.)
[3] 刘学义，任冬莲，李晋明，等.大豆成苗期根毛与抗旱性的关系研究[J].山西农业科学，1996:24(1)：27-30.(Liu X Y，Ren D L，Li J M，et al.Study on the relation between drought-resistance and root hair of soybean in seedling stage[J].Journal of Shanxi Agricultural Sciences，1996，24(1)：27-30.)
[4]刘莹，王锁贵.大豆耐旱种质鉴定及其与根系的相关研究[J].河南农业科学，2006，(9)：48-51.（Lu Y， Wan S G.Identification of drought tolerant germplasm and the correlation with root traits in soybean [glycine max(L）Merr.][J].Journal of Henan Agricultural Sciences，2006，(9)：48-51)
[5]张家榕，李贵全.大豆农艺性状与抗旱性研究[J].山西农业大学学报，2006，26(2)：143-145.（Zhang J R，Li G Q.Studies on agricultural property and drought-resistance of soybean[J].Journal of Shanxi Agricultural University，2006，26(2)：143-145. )
[6]高中超，周宝库，张喜林.大豆对干旱胁迫生理生化的响应[J].大豆通报，2007，(5)：27-30.（Gao Z C，Zhou B K，Zhan X L.The physiology and biochemistry reaction of soybean to drought stress[J].Soybean Bulletin，2007，(5)：27-30.)
[7]莫红，翟兴礼.干旱胁迫对大豆苗期生理生化特性的影响[J].湖北农业科学，2007，46(1)：45-48.(Mo H，Zhai X L.Effects of drought stress on protective enzymes activities and membrane lipid peroxidation in leaves of soybean seedlings[J].Hubei Agricultural Sciences，2007，46(1)：45-48.)
[8] Mian M A R，Bailey M A，Ashley D A，et al.Molecular markers associated with water use efficiency and leaf ash in soybean[J].Crop Science Society of America，1996，36：1252-1257.
[9] Schneider K A，Brothers M E，Kelly J D.Marker assisted selection to improve drought resistance in common bean[J].Crop Science Society of America，1997，37：51-60.
[10]苏乔，刘文哲，吴军，等.东北地区大豆种质资源的RAPD聚类分析[J].植物研究，1998，18(2)：184-188.（Su Q，Liu W Z，Wu J，et al.RAPD analysis of northeast China soybean genotypes[J].Bulletin of Botanical Research，1998，18(2)：184-188.)
[11]赵洪锟，王玉民，李启云，等.中国不同纬度野生大豆和栽培大豆SSR分析[J].大豆科学，2001，20(3)：172-176.(Zhao H K，Wang Y M，Li Q Y，et al.SSR analysis of wild soybean（ G.soja ）and cultivated soybean from different latitude in China[J].Soybean Science，2001，20(3)：172-176.)
[12]林国强，朱海生，黄科.福建栽培大豆品种RAPD标记多样性分析[J].植物遗传资源学报，2005，6(2)：130-134.(Lin G Q，Zhu H S，Huang K.Genetic diversity analysis of soybean cultivars in Fujian province by RAPD[J].Journal of Plant Genetic Resources，2005，6(2)：130-134.)
[13]王振东，孙仓，王惠.不同方法从大豆不同材料中提取基因组DNA效果的比较[J].大豆科学，2008，27(1)：15-19.( Wang Z D，Sun C，Wang H.Comparison of the extraction effect of the soybean genomic DNA from the different materials and different methods[J].Soybean Science，2008，27(1)：15-19.)

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]何? 琳,刘业丽,裴宇峰,等.?2012年黑龙江垦区大豆参试品系纯度鉴定、分子ID构建及遗传多样性分析[J].大豆科学,2013,32(05):591.[doi:10.11861/j.issn.1000-9841.2013.05.0591]
　HE Lin,LIU Ye-li,PEI Yu-feng,et al.Purity Identification,Molecular ID Establishment and Genetic Diversity Analysis of Soybeans Attending Heilongjiang Reclamation Regional Test in 2012[J].Soybean Science,2013,32(01):591.[doi:10.11861/j.issn.1000-9841.2013.05.0591]
[12]张春宝,邱红梅,赵洪锟,等.东北地区大豆种质遗传多样性的SRAP标记分析[J].大豆科学,2014,33(01):17.[doi:10.11861/j.issn.1000-9841.2014.01.0017]
　ZHANG Chunbao,QIU Hongmei,ZHAO Hongkun,et al.Genetic Diversity Analysis of Soybean Germplasm in Northeast Region of China by SRAP Markers[J].Soybean Science,2014,33(01):17.[doi:10.11861/j.issn.1000-9841.2014.01.0017]
[13]何海燕,沙 伟,张艳馥.黑龙江省大豆种质资源遗传多样性的ISSR分析[J].大豆科学,2011,30(01):37.[doi:10.11861/j.issn.1000-9841.2011.01.0037]
　HE Hai-yan,SHA Wei,ZHANG Yan-fu.Genetic Diversity Analysis of Soybean Germplasm in Heilongjiang Province by ISSR Markers[J].Soybean Science,2011,30(01):37.[doi:10.11861/j.issn.1000-9841.2011.01.0037]
[14]王振东,陈超力,于佰双,等.大豆抗旱种质资源遗传多样性的SSR分析[J].大豆科学,2010,29(03):370.[doi:10.11861/j.issn.1000-9841.2010.03.0370]
　WANG Zhen-dong,CHEN Chao-li,YU Bai-shuang,et al.SSR Analysis of Genetic Diversity of Soybean Germplasm in Drought Resistance[J].Soybean Science,2010,29(01):370.[doi:10.11861/j.issn.1000-9841.2010.03.0370]
[15]沙伟,何海燕,张艳馥.黑龙江省大豆品种醇溶蛋白的遗传多样性分析[J].大豆科学,2010,29(06):934.[doi:10.11861/j.issn.1000-9841.2010.06.0934]
　SHA Wei,HE Hai-yan,ZHANG Yan-fu.Gliadin Genetic Diversity in Soybean Varieties in Heilongjiang Province[J].Soybean Science,2010,29(01):934.[doi:10.11861/j.issn.1000-9841.2010.06.0934]
[16]李永春,邢邯,徐冉,等.大豆异地衍生RIL群体部分质量性状的自然选择效应[J].大豆科学,2008,27(04):551.[doi:10.11861/j.issn.1000-9841.2008.04.0551]
　LI Yong-chun,XING Han,XU Ran,et al.Effects of Natural Selection on Partial Qualitative Traits of RIL Populations Developed under Two Ecological Sites Derived from the Combinations of Peking×7605 and RN-9×7605[J].Soybean Science,2008,27(01):551.[doi:10.11861/j.issn.1000-9841.2008.04.0551]
[17]邓莹莹,赵双进,闫龙,等.定向选择对大豆ms1轮回群体遗传基础的影响[J].大豆科学,2015,34(04):548.[doi:10.11861/j.issn.1000-9841.2015.04.0548]
　DENG Ying-ying,ZHAO Shuang-jin,YAN Long,et al.Effect of Orthoselection on Agronomic Traits and Genetic Base of a Male Sterile Soybean Recurrent Population[J].Soybean Science,2015,34(01):548.[doi:10.11861/j.issn.1000-9841.2015.04.0548]
[18]王自力,张吉顺,郭呈宇,等.大豆3个核心亲本及其衍生品系基于PAV分子标记的亲缘关系研究[J].大豆科学,2016,35(01):1.[doi:10.11861/j.issn.1000-9841.2016.01.0001]
　WANG Zi-li,ZHANG Ji-shun,GUO Cheng-yu,et al.Genetic Relationship of Three Soybean Core Parents and Their Derived Breeding Lines Detected by Presence/Absence Variation Markers[J].Soybean Science,2016,35(01):1.[doi:10.11861/j.issn.1000-9841.2016.01.0001]
[19]高晓玲,吴慧,陈琪,等.黄淮海和南方大豆育成品种的目标起始密码子（SCoT）遗传多样性分析[J].大豆科学,2016,35(05):717.[doi:10.11861/j.issn.1000-9841.2016.05.0717]
　GAO Xiao-ling,WU Hui,CHEN Qi,et al.Study on Genetic Diversity of Huang-Huai-Hai and Southern Soybean Cultivars by SCoT Markers[J].Soybean Science,2016,35(01):717.[doi:10.11861/j.issn.1000-9841.2016.05.0717]
[20]周 蓉 　 张小娟 　 王贤智 　 沙爱华 　 周新安 　 涂赣英.湖北省大豆种质资源的遗传多样性分析*[J].大豆科学,2006,25(03):212.[doi:10.11861/j.issn.1000-9841.2006.03.0212]
　Zhou Rong Zhang Xiaojuan Wang Xianzhi Sha Aihua Zhou Xinan T u Ganying.GENETIC DIVERSITY OF SOYBEAN GERMPL ASM FROM HUBEI PROVINCE IN CHINA BY SSE[J].Soybean Science,2006,25(01):212.[doi:10.11861/j.issn.1000-9841.2006.03.0212]

备注/Memo

基金项目：辽宁省博士启动基金资助项目（20071060）；沈阳农业大学青年教师科研基金资助项目（2005006）。

作者简介：王振东（1956-），男，教授，博士生导师，从事生物技术教学与研究。E-mail:zhendongwang1212@yahoo.com.cn。

更新日期/Last Update: 2014-09-29