[1]田军,魏志魁,周先碗.大豆蛋白酶抑制剂的提取及其性质研究[J].大豆科学,2008,27(06):1085-1088.[doi:10.11861/j.issn.1000-9841.2008.06.1085]
　TIAN Jun,WEI Zhi-kui,ZHOU Xian-wan.Extraction and Characterization of Soybean Trypsin Inhibitor[J].Soybean Science,2008,27(06):1085-1088.[doi:10.11861/j.issn.1000-9841.2008.06.1085]
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大豆蛋白酶抑制剂的提取及其性质研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年06期 页码: 1085-1088 栏目: 出版日期: 2008-12-25
Title:
Extraction and Characterization of Soybean Trypsin Inhibitor
文章编号:
1000-9841(2008)06-1085-04
作者:
田军1; 魏志魁2; 周先碗2
1高等教育出版社，北京 100029；
?2北京大学生命科学学院，北京 100871
Author(s):
TIAN Jun1; WEI Zhi-kui2; ZHOU Xian-wan2
1Higher Education Press，Beijing 100029；
2College of Life Sciences，Peking University，Beijing 100871
关键词:
大豆; 胰蛋白酶抑制剂; 等电聚焦电泳
Keywords:
Soybean; Trypsin inhibitor; Isoelectric focusing electrophoresis
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.06.1085
文献标志码:
A
摘要:
蛋白酶抑制剂(Protease Inhibitor)是一类对蛋白酶有抑制作用的多肽或蛋白质，对于生物体内许多重要的生理功能具有调节作用。蛋白酶抑制剂广泛存在于动、植物和微生物中，在豆类植物的储藏器官，特别是种子中含量丰富。在大豆种子中其含量可达总蛋白的6%~8%。且大豆中蛋白酶抑制剂的活性最高。探讨从大豆中提取、分离蛋白酶抑制剂的方法，并对其性质进行初步研究。首先将大豆浸泡液分别通过阴离子和阳离子交换柱层析分离，对获得的各组分进行活性测定、SDS-凝胶电泳和等电聚焦电泳分析。结果显示：最高的组分比活在15 000 U.mg-1左右、相对分子质量在21 000D左右、等电点在4.55左右。
Abstract:
Protease inhibitor is a kind of polypeptides or proteins which has inhibition effect on protease and its highest inhibition activity was observed in the seed of soybean.The soybean trypsin inhibitors were extracted and purified by DEAE-Sepharose 4B FF and SP-Sepharose 4B FF column，and the activities of each obtained component were measured.Meanwhile，SDS-PAGE and isoelectric focusing electrophoresis were also used to determine the relative molecular weight and isoelectric point.Result showed the component with the highest inhibition activity has the specific inhibition activity of 15 000 U.mg-1，the Mr?of 21 000 and the isoelectric point of 4.55.

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备注/Memo

作者简介：田军，女（1966-），在读硕士, 副编审。E-mail： tianjun@hep.com.cn。

更新日期/Last Update: 2014-10-06