[1]吴丽丽 唐月异赵洪锟,董英山,李启云,等.大豆细胞质COXⅡ基因的分离及序列分析[J].大豆科学,2008,27(06):933-935.[doi:10.11861/j.issn.1000-9841.2008.06.0933]
　WU Li-li,TANG Yue-yi,ZHAO Hong-kun,et al.Isolation and Sequence Analysis of COXⅡGene in Soybean Cytoplasm[J].Soybean Science,2008,27(06):933-935.[doi:10.11861/j.issn.1000-9841.2008.06.0933]
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大豆细胞质COXⅡ基因的分离及序列分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年06期 页码: 933-935 栏目: 出版日期: 2008-12-25
Title:
Isolation and Sequence Analysis of COXⅡGene in Soybean Cytoplasm
文章编号:
1000-9841(2008)06-0933-03
作者:
吴丽丽1; 2 唐月异1; 3赵洪锟1; 董英山1; 李启云1; 赵丽梅1; 王庆胜2
1吉林省农业科学院，吉林 长春130033；
2黑龙江省农业科学院佳木斯分院，黑龙江 佳木斯154007；
3山东省花生研究所，山东 青岛266000
Author(s):
WU Li-li1; 2; TANG Yue-yi1; 3; ZHAO Hong-kun1; DONG Ying-shan1; LI Qi-yun1; ZHAO Li-mei2; WANG Qing-sheng2
1 Jilin Academy of Agricultural Sciences，Changchun 130033,Jilin；
2 Jiamusi Branch of Heilongjiang Academy of Agricultural Sciences，Jiamusi 154007，Heilongjiang；
3Shandong Peanut Research Institute，Qingdao 266000，Shandong，China
关键词:
大豆; 细胞质雄性不育;  COXⅡ基因
Keywords:
Soybean; Cytoplasmic male sterility（CMS）; COXⅡ gene
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.06.0933
文献标志码:
A
摘要:
目前关于细胞质雄性不育的机理研究主要集中在植物线粒体上，通过参考大豆线粒体细胞色素氧化酶亚基Ⅱ（COXⅡ）基因序列，合成保守基因引物，从供试材料中克隆到了COXⅡ基因，对基因序列分析表明：该基因编码了一个有280个氨基酸组成的蛋白，推测其可能与大豆细胞质不育有关。
Abstract:
At present，the research about the mechanism of CMS are all focusing on mitochondrial genome analysis，A pair of primers was designed according to the soybean Mitochondrial cytochrome oxidase subunitⅡsequence which published in GenBank.The gene was isolated and analysed from the CMS soybean.This gene codes a protein which consisting 280 amino acids，and maybe assosiated with CMS.

参考文献/References:

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备注/Memo

基金项目：国家自然科学基金资助项目(30370890,30490250)；国家高技术研究发展计划（863计划）资助项目(2006AA10Z120)。

作者简介：吴丽丽（1981-），女，研究实习员，硕士，主要从事玉米育种研究。E-mail:lili_0451@yahoo.com.cn。

更新日期/Last Update: 2014-10-04