[1]张颖君,高慧敏,蒋春志,等.大豆种子脂肪酸含量的快速测定[J].大豆科学,2008,27(05):859-862.[doi:10.11861/j.issn.1000-9841.2008.05.0859]
　ZHANG Ying-jun,GAO Hui-min,JIANG Chun-zhi,et al.Fast Analysis on Fatty Acids of Soybean Seed by Gas Chromatography[J].Soybean Science,2008,27(05):859-862.[doi:10.11861/j.issn.1000-9841.2008.05.0859]
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大豆种子脂肪酸含量的快速测定

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年05期 页码: 859-862 栏目: 出版日期: 2008-10-25
Title:
Fast Analysis on Fatty Acids of Soybean Seed by Gas Chromatography
文章编号:
1000-9841(2008)05-0859-04
作者:
张颖君1; 高慧敏2; 蒋春志1; 胡梦芸1; 刘兵强1; 李辉1
1河北省农林科学院粮油作物研究所，河北省作物遗传育种实验室，国家大豆改良中心石家庄分中心，河北 石家庄；
2河北省农林科学院经济作物研究所，河北 石家庄 050031
Author(s):
ZHANG Ying-jun1; GAO Hui-min2; JIANG Chun-zhi1; HU Meng-yun1; LIU Bing-qiang; LI Hui1
1 Institute of Cereal and Oil Crops，Hebei Academy of Agriculture and Forestry Sciences，Key Laboratory of Crop Genetics and Breeding of Hebei Province，National Soybean Improvement Center Shijiazhuang Sub-center,Shijiazhuang 050031；
2Cash and Crops Institute of Agriculture，Hebei Academy of Agriculture and Forestry Sciences，Shijiazhuang 050031，Hebei，China
关键词:
大豆; 脂肪酸; 气相色谱
Keywords:
Soybean; Fatty acids; Gas chromatography
分类号:
Q547
DOI:
10.11861/j.issn.1000-9841.2008.05.0859
文献标志码:
A
摘要:
脂肪酸的含量是油料作物品质的重要指标，以往的脂肪酸测定方法需要样品量大、工序繁琐、耗时长，不适合育种工作者前期对材料的筛选。用0.040 g大豆种子（约占种子的1/4）对脂肪酸的提取和甲酯化过程进行了条件优化，采用提取液（苯：石油醚＝1：1）提取5 min，0.5 mol.L-1甲醇钠酯化7 min后即可进行气相色谱分析，使通常需要用近10 h的工作在12 min内完成，大大缩短了检测时间。通过与传统的索氏提取(GB/T 5009.6-2003)、甲酯化方法(GB/T 17376-1998)比较，改进的方法结果准确可靠。
Abstract:
The content of fatty acids is an important index for oil crops.The traditional methods of detecting fatty acids need more samples and more time，which unsuitable to select hundreds of seeds for breeders.In this experiment，0.040 g soybean seed (about quarter of the soybean seed) was used to optimize the method of oil extraction and methyl esterification，and extracted oil from the soybean powder using the mixture (benzene：petroleum ether=1：1) for 5 minutes，methyl esterificated using sodium hydroxide-methanol solution (c= 0.5 mol.L-1) for 7 minutes，then the supernatant fluid was injected into the gas chromatography system.Using this method，the work which usually need almost ten hours，now can be completed in 12 minutes，thus lots of time can be saved.By comparing with traditional method (GB/T 5009.6-2003，GB/T 17376-1998) for detecting fatty acid compositions，it was confirmed that the results got from this method was exact and credible.

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备注/Memo

作者简介：张颖君（1977-），男，助理研究员，硕士，主要从事作物品质育种与生物技术研究。E-mail:zhang-yingjun@sohu.com。

通讯作者：李辉，研究员。E-mail:zwslihui@163.com

更新日期/Last Update: 2014-10-06