[1]孟庆杰,许艳丽,李春杰,等.不同施肥/土地利用方式对黑土细菌多样性的影响[J].大豆科学,2008,27(03):480-486.[doi:10.11861/j.issn.1000-9841.2008.03.0480]
　MENG Qing-jie,XU Yan-li,LI Chun-jie,et al.Effects of Different Fertilization and Land Use History on the Bacterial Diversity in Black Soils[J].Soybean Science,2008,27(03):480-486.[doi:10.11861/j.issn.1000-9841.2008.03.0480]
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不同施肥/土地利用方式对黑土细菌多样性的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年03期 页码: 480-486 栏目: 出版日期: 2008-06-25
Title:
Effects of Different Fertilization and Land Use History on the Bacterial Diversity in Black Soils
文章编号:
1000-9841(2008)03-0480-07
作者:
孟庆杰1; 2; 许艳丽2; 李春杰2; 韩晓增2; 裴希超1; 2
1东北林业大学生命科学学院，黑龙江 哈尔滨 150040；
2中国科学院东北地理与农业生态研究所，黑龙江 哈尔滨 150081
Author(s):
MENG Qing-jie1; 2; XU Yan-li2; LI Chun-jie2; HAN Xiao-zeng2; PEI Xi-chao1; 2
1 College of Life Science，Northeast Forestry University，Harbin 150040，Heilongjiang；
2 Northeast Institute of Geography and Agricultural Ecology，CAS，Harbin 150081，Heilongjiang，China
关键词:
大豆; DGGE; 黑土; 细菌多样性; 施肥; 土地利用
Keywords:
Soybean; DGGE; Black soil; Bacterial diversity; Fertilization; Land use
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.03.0480
文献标志码:
A
摘要:
微生物多样性是表征土壤质量变化的敏感指标。为探讨黑土生态系统转变过程中土壤微生物多样性变化，应用变性梯度凝胶电泳（DGGE）方法对黑土区3种生态系统和豆田3种施肥制度下土壤细菌多样性进行了初步研究。结果表明：土壤细菌多样性以草地（GE）最高，其细菌丰富度（S）和Shannon多样性指数均高于农田（CK）和裸地（BE）；在豆田土壤中，长期不施肥(CK）的土壤细菌丰富度和Shannon多样性指数最高，其次为长期化肥配施有机肥（NPM），长期单施化肥（NP）最低。UPGMA聚类分析表明，NP与CK处理细菌的群落结构相似，相似性系数达到65.5%，NPM与GE和BE处理细菌的群落结构相似，相似性系数分别为52.5%和57.3%。
Abstract:
Soil managements such as fertilization and land use history can affect soil quality，and the whilst biology diversity is a sensitive indicator of it.To discuss the change of soil microbial diversity with the evolvement of the soil ecosystems，the method of denaturing gradient gel electrophoresis(DGGE) was applied to study the diversity of soil bacteria in the three black soil ecosystems and soybean fields which were applied three fertilizers.The results showed that the bacteria diversity of grassland ecosystem(GE)is the highest，and the bacteria richness(S) and shannon diversity index were higher than those of agricultural ecosystem(CK) and bare land ecosystem(BE)；soil bacteria richness and shannon diversity index of soybean fields with no fertilization for the long-term(CK) were the highest，followed by chemical fertilizers for the long-term with farmyard manure(NPM)，those of the application of chemical fertilizer(NP)alone were the lowest，respectively.UPGMA clustering analysis showed that bacterial communities of CK and NP belonged to one cluster，the similarity coefficient was 65.5%，that bacterial communities of NPM was similar to that of GE and BE，the similarity coefficients were 52.5% and 57.3%，respectively.

参考文献/References:

[1]钟文辉，蔡祖聪.土壤管理措施及环境因素对土壤微生物多样性影响研究进展[J].生物多样性，2004，12(4)：456-465.(Zhong W H，Cai Z C.Effect of soil management practices and environmental factors on soil microbial diversity：a review[J].Biodiversity Science，2004，12(4)：456-465.)

[2]黎宁，李华兴，朱凤娇，等.菜园土壤微生物生态特征与土壤理化性质的关系[J].应用生态学报，2006，17(2)：285-290.(Li N，Li H X，Zhu F J，et al.Relationships between soil microbial ecological characteristics and physical-chemical properties of vegetable garden soil[J].Chinese Journal of Applied Ecology，2006，17(2)：285-290.)
[3]魏丹，杨谦，迟凤琴.东北黑土区土壤资源现状与存在问题[J].黑龙江农业科学，2006(6)：69-72.(Wei D，Yang Q，Chi F Q.The soil resource conditions and the problems in northeast black soil regions[J].Heilongjiang Agricultural Sciences，2006(6)：69-72.)
[4]陈溯，王占哲.黑土区大豆高效组合施肥技术试验研究[J].农业系统科学与综合研究，2001，17(3)：221-222.(Chen S，Wang Z Z.The study on techniques of assemble fertilization for soybean in black soil area[J].System Sciences and Comprehensine Studies in Agriculture，2001，17(3)：221-222.)
[5]索东让，王平.长期定位施肥对土地生产力的影响[J].西北农业学报，2000，9(3)：72-75.(Suo D R，Wang P.Effect of long-term located fertilization on land productivity[J].Acta Agricuhurae Boreali-occidentalis Sinica，2000，9(3)：72-75.)
[6]邱多生，李恋卿，焦少俊，等.长期不同施肥下太湖地区黄泥土肥力的变化[J].土壤肥料，2005，(4)：28-32.(Qiu D S，Li L Q，Jiao S J，et al.Change of soil fertility under long-term different fertilization practices in a paddy soil from the Tai Lake region[J].Soil and Fertilizers，2005(4)：28-32.)
[7]樊军，郝明德.长期轮作与施肥对土壤主要微生物类群的影响[J].水土保持研究，2003，10(1)：88-89，114.(Fan J，Hao M D.Effects of long-term rotations and fertilizations on soil microflora[J].Research of Soil and Water Conservation，2003，10(1)：88-89，114.)
[8]王光华，齐晓宁，金剑，等.施肥对黑土农田土壤全碳、微生物量碳及土壤酶活性的影响[J].土壤通报，2007，38(4)：661-666.(Wang G H，Qi X N，Jin J，et al.Effect of fertilization on total soil C，microbial biomass C and soil enzyme activities in farmland black soil[J].Chinese Journal of Soil Science，2007，38(4)：661-666.)
[9]徐华勤，肖润林，邹冬生，等.长期施肥对茶园土壤微生物群落功能多样性的影响[J].生态学报，2007，27(8)：3355-3361.(Xu H Q，Xiao R L，Zou D S，et al.Effects of long-term fertilization on functional diversity of soil microbial community of the tea plantation[J].Acta Ecologica Sinica，2007，27(8)：3355-3361.)
[10]刘鸿翔，王德禄，王守宇，等.黑土长期施肥及养分循环再利用的作物产量及土壤肥力质量变化Ⅲ.土壤养分收支[J].应用生态学报，2002，13(11)：1410-1412.(Liu H X，Wang D L，Wang S Y，et al.Changes of crop yield and soil fertility under long-term application of fertilizer and recycled nutrients in manure on a black soiI.SoiI nutrient budget[J].Chinese Journal of Applied Ecology，2002，13(11)：1410-1412.)
[11]董炳友，高淑英，吕正文.不同施肥措施对连作大豆的产量及土壤pH值的影响[J].黑龙江八一农垦大学学报，2002，14(4)：19-21.(Dong B Y，Gao S Y，Lü Z W.Effect of different fertilizing on soybean yield and soil pH value in continuous cropping[J].Heilongjiang August First Land Reclamation University，2002，14(4)：19-21.)
[12]韩晓增，王守宇，宋春雨，等.土地利用／覆盖变化对黑土生态环境的影响[J].地理科学，2005，25(2)：203-208.（Han X Z，Wang S Y，Song C Y，et al.Effects of land use and cover change on ecological environment in black soil region[J].Scientia Geographica Sinica，2005，25(2)：203-208.)
[13]隋跃宇，焦晓光，张兴义，等.不同施肥制度对大豆生育期土壤微生物量的影响[J].土壤通报，2006，37(5)：894-896.(Sui Y Y，Jiao X G，Zhang X Y，et al.Effect of different fertilizer application strategies on soil microbial biomass during soybean growth periods[J].Chinese Joumal of Soil Science，2006，37(5)：894-896.)
[14]刘新晶，许艳丽，李春杰，等.大豆轮作系统对土壤细菌生理菌群的影响[J].大豆科学，2007，26(5)：723-727.(Liu X J，Xu Y L，Li C J，et al.Effect of soybean rotation system on the bacterial physiological groups[J].Soybean Science，2007，26(5)：723-727.)
[15]刘强，邓伟，韩晓增，等.海伦黑土区农田水分动态平衡与数值模拟[J].农业系统科学与综合研究，2005，21(3)：185-189.（Liu Q，Deng W，Han X Z，et al.The dynamic balance on the black soil moisture and it’s numerical simulation in Hailun district[J].System Sciences and Comprehensive Studies In Agriculture，2005，21(3)：185-189.)
[16]王风，韩晓增，李海波，等.不同黑土生态系统的土壤水分物理性质研究[J].水土保持学报，2006，20(6)：67-70.(Wang F，Han X Z，Li H B，et al.Hydro-physical properties of black soil in different type of eco-system[J].Journal of Soil and Water Conservation，2006，20(6)：67-70.)
[17]Muyzer G，de Waal E C，Uitterlinden A G.Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction amplified genes encoding for 16S rRNA[J].Applied and Enviromnental Microbiology，1993，59：695-700.
[18]Miller D N，Bryant J E，Madsen E L，et al.Evaluation and optimization of DNA extraction and purification procedures for soil and sediment samples[J].Applied and Enviromnental Microbiology，1999，65(11)：4715-4724.
[19]罗海峰，齐鸿雁，薛凯，等.PCR-DGGE技术在农田土壤微生物多样性研究中的应用[J].生态学报，2003，23(8)：1570-1575.（Luo H F，Qi H Y，Xue K，et al.A preliminary application of PCR-DGGE to study microbial diversity in soil[J].Acta Ecologica Sinica，2003，23(8)：1570-1575.)
[20]罗海峰，齐鸿雁，张洪勋.乙草胺对农田土壤细菌多样性的影响[J].微生物学报，2004，44(4)：519-522.(Luo H F，Qi H Y，Zhang H X.The impact of acetochl or the bacterial diversity in soil[J].Acta Microbiologica Sinica，2004，44(4)：519-522.)
[21]赵兴青，杨柳燕，陈灿，等.PCR-DGGE技术用于湖泊沉积物中微生物群落结构多样性研究[J].生态学报，2006，3(26)：3610-3616.(Zhao X Q，Yang L Y，Chen C，et al.Study on microbial diversity in lake sediments by the method of PCR-DGGE[J].Acta Ecologica Sinica，2006，3(26)：3610-3616.)
[22]Zhou J，Bruns M A，Tiedje J M.DNA recovery from soils of diverse composition[J].Applied and Environmental Microbiology，1996，62：316-322.
[23]薛冬，姚槐应，黄昌勇.茶园土壤微生物群落基因多样性[J].应用生态学报，2007，18(4)：843-847.(Xue D，Yao H Y，Huang C Y.Genetic diversity of microbial communities in tea orchard soil[J].Chinese Journal of Applied Ecology，2007，18(4)：843-847.)
[24]张平究，李恋卿，潘根兴，等.长期不同施肥下太湖地区黄泥土表土微生物碳氮量及基因多样性变化[J].生态学报，2004，24(12)：2818-2814.(Zhang P J，Li L Q，Pan G X，et al.Influence of long-term fertilizer management on top soil microbial biomass and genetic diversity of a paddy soil from the Tai Lake region[J].Acta Ecologica Sinica，2004，24(12)：2818-2814.)
[25]Gelsomino A，Keijzer-Wolters A C，Cacco G，et al.Assessment of bacterial community structure in soil by polymerase chain reaction and denaturing gradient gel electrophoresis[J].Journal of Microbiological Methods，1999，38：1-15.
[26]Schutter M E，Sandeno J M，Dick R P.Seasonal，soil type，and alter native management influences on microbial communities of vegetable cropping systems[J].Biology and Fertility of Soils，2001，34：397-410.
[27]赵丽娟，韩晓增，王守宇，等.黑土长期施肥及养分循环再利用的作物产量及土壤肥力变化Ⅳ.有机碳组分的变化[J].应用生态学报，2006，17(5)：817-821.(Zhao L J，Han X Z，Wang S Y，et al.Changes of crop yield and soil fertility under long-term fertilization and nutrients-recycling and reutilization on a black soil IV.Soil organic carbon and its fractions[J].Chinese Journal of Applied Ecology，2006，17(5)：817-821.)
[28]黄进勇，李春霞.土壤微生物多样性的主要影响因子及其效应[J].河南科技大学学报(农学版)，2004，24(4)：10-13.(Huang J Y，Li C X.Main factors of soil microbial diversity and their effect on cropland[J].Journal of Henan University of Science and Technology(Agricultural Science)，2004，24(4)：10-13.)
[29]van Elsas J D，Garbeva P，Salles J.Effects of agronomical measures on the microbial diversity of soils as related to the suppression of soil-borne plant pathogens[J].Biodegradation，2002，13：29-40.
[30]Ekundayo E O.Effect of common pesticides used in the Niger delta basin of southern Nigeria on soil microbial populations[J].Environmental Monitoring and Assessment，2003，89：35-41.
[31]胡元森，刘亚峰，吴坤，等.黄瓜连作土壤微生物区系变化研究[J].土壤通报，2006，37：126-129.(Hu Y S，Liu Y F，Wu K，et al.Variation of microbial community structure in relation to successive cucumber cropping soil[J].Chinese Journa1 of Soil Science，2006，37：126-129.)
[32]Yang Y H，Yao J，Hu S，et al.Effects of agricultural chemicals on DNA sequence diversity of soil microbial community：a study with RAPD marker[J].Microbial Ecology，2000，39：72-79.
[33]Sarathchandra S U，Ghani A，Yeates G W，et al.Effect of nitrogen and phosphate fertilisers on microbial and nematode diversity in pasture soils[J].Soil Biology and Biochemistry，2001，33：953-964.

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备注/Memo

基金项目：中国科学院知识创新工程重要方向资助项目(kzcx2-yw-408)；国家“十一五”科技支撑资助项目（GA06B101）。

作者简介：孟庆杰（1982-），女，硕士研究生，主要从事土壤微生物多样性研究。E-mail: mengqingjie6646499@163.com。

更新日期/Last Update: 2014-10-08