[1]刘亚光,赵滨,马超.水杨酸和壳聚糖诱导大豆对灰斑病的抗性[J].大豆科学,2008,27(02):298-300.[doi:10.11861/j.issn.1000-9841.2008.02.0298]
　LIU Ya-guang,ZHAO Bin,MA Chao.Induction effect of Salicylic acid and Chitosan on Frogeye Leaf Spot in Soybean[J].Soybean Science,2008,27(02):298-300.[doi:10.11861/j.issn.1000-9841.2008.02.0298]
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水杨酸和壳聚糖诱导大豆对灰斑病的抗性

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年02期 页码: 298-300 栏目: 出版日期: 2008-04-30
Title:
Induction effect of Salicylic acid and Chitosan on Frogeye Leaf Spot in Soybean
文章编号:
1000-9841(2008)02-0296-05
作者:
刘亚光1; 赵滨2; 马超1
1东北农业大学农学院，黑龙江 哈尔滨 150030;
2黑龙江省植保站，黑龙江 哈尔滨150090
Author(s):
LIU Ya-guang1; ZHAO Bin2; MA Chao1
1College of Agriculture，Northeast Agricultural University,Harbin 150030，Heilongjiang；
2Heilongjiang Plant Protection Station，Harbin 150090，Heilongjiang，China
关键词:
大豆; 大豆灰斑病; 诱导抗性; 水杨酸; 壳聚糖
Keywords:
Frog-eye leaf spot; Induced resistance; Salicylic acid; Chitosan
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.02.0298
文献标志码:
A
摘要:
植物诱导抗病性是植物保护的一项新措施，灰斑病是大豆的重要病害之一，目前生产中主要是推广抗病品种进行预防。而有关诱导大豆抗灰斑病的研究报道极少，本研究主要是利用两种常见的化学诱导因子来探讨对大豆抗灰斑病的诱抗效果，为进一步开发高效、安全、环保的诱抗剂奠定理论基础。选用水杨酸(SA)和壳聚糖两种诱抗剂处理感病品种黑农35和中抗品种东农42，当大豆第4片复叶完全展开时，分别在当天和以后的5，10 d对大豆植株进行不同的诱导处理。结果表明，当水杨酸浓度为1 000 mgL-1时连续叶喷三次，使黑农35和东农42的诱抗效果分别达到了72.2%和66.7%；浓度为1 000 mgL-1的壳聚糖叶喷对黑农35和东农42的诱抗效果分别达到了72.2％和55.6％；而10 000 mgL-1壳聚糖溶液浸种与1 000 mgL-1壳聚糖溶液叶喷混用对于黑农35和东农42诱导作用最强，诱抗效果分别可达88.9%和72.2%。说明水杨酸和壳聚糖均能够诱导大豆品种（黑农35和东农42）对灰斑病产生较高的抗性。诱抗效果因所用试剂的浓度及作物品种而异，施用方法以及施用次数也影响诱抗效果，水杨酸在适宜浓度下对黑农35的诱抗效果明显高于东农42。
Abstract:
Plant induced resistance is a new measure of plant protection，Frog-eye leaf spot，caused by Cercospora sojina Hara，is one of important diseases of soybean.Resistant soybean varieties were mainly used to resistance to frog-eye leaf spot at present soybean production, while the study on induced resistance of soybean to frog-eye leaf spot is rarely reported.Two common chemistry induced factors，salicylic acid and chitosan，were used to discuss the effects of induced resistance to frog-eye leaf spot. Two soybean varieties，Heinong 35 (susceptible) and Dongnong 42 (middle resistance)，were used as experiment material.For salicylic acid(SA)，the seeds were sowed in the pots.When the 4th compound leaf of soybean spreaded completely，SA sprayed with 3 concentration at the same day and 5 days and 10 days later.But for chitosan，seed soaking，seed coating，spraying and mixture of above were used.3 concentrations for each treatment were used.The spraying time of chitosan was the same as that of SA .The pathogen of frog-eye leaf spot were inoculated at 5 days and 12 days after the last treatment.The progressions of disease spots were investigated at 15 days after inoculation.Results showed that all the treatments of SA and chitosan reached significant level.The induced resistance of SA reached 72.2% and 66.7% for Heinong 35 and Dongnong 42，respectively.The best concentration by leaf spraying three times of SA was 1 000 mgL-1.In addition，the induced resistance effects among all treatments of chitosan reached 88.9% and 72.2% for Heinong 35 and Dongnong 42，respectively，when seed soaking by 10 000 mgL-1Chitosan solution mixed with leaf spraying by 1 000 mgL-1Chitosan solution. Then，the induced resistance effects of Chitosan reached 72.2% and 55.6% for Heinong 35 and Dongnong 42，respectively，when Chitosan concentration was 1 000 mgL-1?by spraying three times. Whereas the induced resistacne effects was the lowest by seed soaking.The conclusion of the research is that two inducers could both induce higher resistance to frog-eye leaf spot in Heinong 35 and Dongnong 42，but there were some difference between the two genotypes.The effects of induced resistance of Heinong 35 were obviously higher than that of Dongnong 42, when the concentration of SA was appropriate. However，this difference such as the former was not obvious among all the treatments induced by chitosan.These factors including inducer concentrations，spraying times and application methods all affected the effect of induced resistance.The the best treatment is seed soaking mixing with leaf spraying three times by 1 000 mgL-1Chitosan solution.

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备注/Memo

基金项目：大豆生物学教育部重点实验室主任基金资助项目（SB05B01）；哈尔滨市科技局学科后备带头人基金资助项目（2004AFXXJ049）。

作者简介：刘亚光（1968-），女，副教授，博士，现主要从事植物保护方向的教学与科研。Tel：0451-55191069；Fax：0451-55190447；E-mail：Liuyaguang@sina.com。

更新日期/Last Update: 2014-10-10