[1]金剑,王光华,陈雪丽,等.Biolog-ECO解析不同大豆基因型R1期根际微生物群落功能多样性特征[J].大豆科学,2007,26(04):565-570.[doi:10.3969/j.issn.1000-9841.2007.04.022]
　JIN Jian,WANG Guang-hua,CHEN Xue-li,et al.ANALYSIS OF MICROBIAL COMMUNITY FUNCTIONAL DIVERSITY IN RHIZOSPHERE OF DIFFERENT SOYBEAN GENOTYPES AT R1 STAGE USING BIOLOG-ECO METHOD[J].Soybean Science,2007,26(04):565-570.[doi:10.3969/j.issn.1000-9841.2007.04.022]
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Biolog-ECO解析不同大豆基因型R1期根际微生物群落功能多样性特征

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第26卷 期数: 2007年04期 页码: 565-570 栏目: 出版日期: 2007-08-25
Title:
ANALYSIS OF MICROBIAL COMMUNITY FUNCTIONAL DIVERSITY IN RHIZOSPHERE OF DIFFERENT SOYBEAN GENOTYPES AT R1 STAGE USING BIOLOG-ECO METHOD
文章编号:
1000-9841(2007)04-0565-06
作者:
金剑1; 2; 王光华1; 陈雪丽1; 刘晓冰1 ; Stephen J Herbert3
1中国科学院东北地理与农业生态研究所黑土重点实验室，哈尔滨 150081；
2大豆生物学教育部重点实验室，哈尔滨 150030；
3美国麻省大学植物与土壤科学系，阿姆斯特市 01003，美国
Author(s):
JIN Jian1; 2 ; WANG Guang-hua1; CHEN Xue-li1; LIU Xiao-bing1;  Stephen J Herbert3
1Key Lab of Black Soil，Northeast Institute of Geography and Agro-ecology，Chinese Academy of Science，Harbin 150081；
2Key Laboratory of Soybean Biology of Ministry of Education，Harbin 150030；
3Department of Plant and Soil Sciences，University of Massachusetts，Amherst 01003，MA，USA
关键词:
大豆; 根际; AWCD; 微生物多样性; 黑土
Keywords:
Soybean; Rhizosphere; AWCD; Microbial diversity; Mollisol
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2007.04.022
文献标志码:
A
摘要:
利用Biolog-Eco技术对2个不同大豆基因型R1期的根际和非根际微生物群落功能多样性进行研究。结果表明，大豆根际微生物利用碳源量在整个培养过程都显著高于非根际，根际微生物的生理活性显著地高于非根际；不同基因型大豆对根际微生物群落功能多样性有显著影响，高产大豆北丰11根际微生物总体表现较高的活性，而且根际微生物利用碳源的类型有别于海9731，其利用羧酸类和多聚物相对较多，如α-丁酮酸、4-羟基苯甲酸、衣康酸、肝糖、吐温40和吐温80；而海9731利用氨基酸类及胺/氨类碳源较多，如腐胺和L-精氨酸。
Abstract:
The microbial community functional diversity in rhizosphere and bulk soil of two soybean genotypes at R1 stage was investigated with the Biolog method.The results showed that the microbe in rhizosphere utilized more carbons during the entire incubation period，compared with bulk soil，which indicated that the metabolism activity of microbe in rhizosphere was high；soybean genotype significantly influenced the microbial community functional diversity and the microbe in rhizosphere of Beifeng 11 had relatively higher activity of metabolism than that of Hai 9731.Furthermore，the category of carbon utilized by microbial community in rhizosphere of Beifeng 11 was different from Hai 9731.Beifeng 11 prefered carboxylic acids and polymers such as α.Keto butyric acid,4-Hydroxy benzoic acid，itaconic acid，glycogen，tween 40，tween 80,while Hai 9731 prefered amino acids and amines such as putrescine and L-arginine.

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备注/Memo

基金项目：国家自然科学基金（40541004）和黑龙江省杰出青年基金（JC200617）

作者简介：金剑（1974-），男，副研，博士，主要从事黑土区作物生理生态研究。

更新日期/Last Update: 2014-10-20