[1]吴家林,张敬平,胡遴,等.大豆和玉米加工产品中外源转基因成分检测技术的建立[J].大豆科学,2007,26(01):84-88.[doi:10.3969/j.issn.1000-9841.2007.01.019]
　WU Jia-lin,ZHANG Jing-ping,HU Lin,et al.DETECTION OF GENETICALLY MODIFIED COMPONENTS IN PRODUCTS DERIVED FROM SOYBEAN AND MAIZE[J].Soybean Science,2007,26(01):84-88.[doi:10.3969/j.issn.1000-9841.2007.01.019]
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大豆和玉米加工产品中外源转基因成分检测技术的建立

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第26卷 期数: 2007年01期 页码: 84-88 栏目: 出版日期: 2007-02-25
Title:
DETECTION OF GENETICALLY MODIFIED COMPONENTS IN PRODUCTS DERIVED FROM SOYBEAN AND MAIZE
文章编号:
1000-9841(207)1-0084-05
作者:
吴家林; 张敬平; 胡遴; 钮伟民; 尤凤兴
江苏省无锡市疾病预防控制中心，无锡 214002
Author(s):
WU Jia-lin; ZHANG Jing-ping; HU Lin; NIU Wei-min; YOU Feng-xing
Wuxi Center for Disease Control and Prevention，Wuxi 214002
关键词:
大豆; 玉米; 加工产品; PCR检测; 转基因成分
Keywords:
Soybean; Maize; Processed products; Qualitative PCR; Genetically modified Components
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2007.01.019
文献标志码:
A
摘要:
采用PCR技术检测 CaMV 35S 启动子，并进一步通过PCR 检测 RoundUp Ready Soybean(RRS) 和 Bt176 Maximaizer 的特异性DNA 片段，判断大豆和玉米加工产品中是否含相应转基因成分。在1份豆粕和豆腐样品中检测到了 RoundUp Ready 大豆特异性的498 bp片段，而在玉米粒样品中检测到了 Bt176 特异性转基因成分。PCR检测的灵敏度达到0.1%，稳定性良好。结果表明，PCR技术检测外源基因是灵敏和准确的，可以广泛地应用到转基因作物及其加工产品的转基因成分检测中。
Abstract:
The purpose of this study was to establish detection method on genetically modified components in products derived from soybean and maize using PCR technology. The presence of processed products were investigated by PCR detection of CaMV 35S promoter， and the presence of Round up Ready Soybean (RRS)， Bt176 Maximaizer in products derived from soybean and maize were further determined to detect their specific DNA fragments respectively by PCR. One bean cake sample and one bean curd sample were verified a 498bp RRS specific DNA fragment by PCR detection， Bt176 Maximaizer specific PCR products were obtained with the corn grain DNA samples used as PCR templates.The research also succeeded in settling 0.1% precision degree of genetically modified organisms which ensured its stable property. The results showed that PCR analysis for the transgene was feasible and reliable. The PCR analysis of transgenic components could be widely used in various transgenic plants and their products.

参考文献/References:

［1］Clive James. Global Status of Commercialized Biotech/GM Crops：2005，http：//www.isaaa.org.

［2］Lipp M，Brodmann P，Pietsch K，et al.IUPAC collaborative trial study of a method to detect genetically modified soybeans and maize in dried powder［J］. J AOAC Int，1999，82(4)：923-928.
［3］Hurst CD， Knight A， Bruce IJ. PCR detection of genetically modified soya and maize in food stuffs ［J］. Molecular Breeding， 1999， （5）： 579-586.
［4］Jankiewicz A，Broll H，Zagon J.The official method for the detection of the genetically modified soybeans (German food act LMBG 35). A semi-quantitative study of sensitivity limits with glyphosate-tolerant soybeans(Roundup Ready) and insect-resistance Bt maize (Maximizer) ［J］.European Food Research and Technology,1999，209：77-82.
［5］陈颖，王媛，徐宝梁,等.食品加工工艺对大豆内源基因降解变化规律的影响［J］.中国粮油学报,2005，20(4)：60-64.

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备注/Memo

基金项目：江苏省预防医学科研基金资助项目（YZ200418）

作者简介：吴家林（1973-），男，硕士，主管技师，研究方向为转基因食品检测研究；张敬平（1957-），男，副主任医师，研究方向为预防医学与公共卫生管理。吴家林、张敬平为共同第一作者。

更新日期/Last Update: 2014-10-21