[J].Soybean Science,2006,25(03):244-249.[doi:10.11861/j.issn.1000-9841.2006.03.0244]
大豆 SMV 3 号株系抗病基因的 SSR 标记*
- 文献标志码:
- A
- 摘要:
- 运用简单序列重复技术(SSR 技术),采用改良的分离群体组群分析法(BSA 法), 对大豆品系中选 95- 5117 (R)×HB1(S)的 F 5 代重组自交系群体接种 SMV 3 号株系鉴定抗性 ,并进行抗病基因的分子定位。结果表明 :中选 95 - 5117 对 SMV 3 号株系的抗性受一对基因控制。用Mapmaker /Exp3. 0b 进行连锁分析,该基因位于大豆染色体组的 F 连锁群上, 并获得了与 SMV3号株系抗病基因连锁的 2 个 SSR 标记 Satt114 和 Satt362,遗传距离分别为 2. 3cM 和 8. 6cM 。标记与抗病基因间的排列顺序和距离为:Satt114 - 2. 3 cM - RSMV3 - 8. 6 cM - Satt362。
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备注/Memo
基金项目:863 项目(编号:2003AA207060)的部分研究内容