[1]麻 　浩 　王显生 　刘　春　俞　阗　郝小燕　高文瑞　何小铃.706 份中国大豆种质贮藏蛋白 7S 和 11S 组分及其亚基相对含量的研究[J].大豆科学,2006,25(01):11-17.[doi:10.11861/j.issn.1000-9841.2006.01.0011]
　Ma Hao -Wang Xiansheng -Liu Chun -Yu Tian -Hao XiaoyanGao Wenrui -He Xiaoling.The Content Variation of 7S ,11S Globulins andTheir Subunits of Seed Storage Protein of706 Chinese Soybean Germplasm*[J].Soybean Science,2006,25(01):11-17.[doi:10.11861/j.issn.1000-9841.2006.01.0011]
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706 份中国大豆种质贮藏蛋白 7S 和 11S 组分及其亚基相对含量的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第25卷 期数: 2006年01期 页码: 11-17 栏目: 出版日期: 2006-02-25
Title:
The Content Variation of 7S ,11S Globulins andTheir Subunits of Seed Storage Protein of706 Chinese Soybean Germplasm*
作者:
麻 　浩 　王显生 　刘　春　俞　阗　郝小燕　高文瑞　何小铃
(南京农业大学作物遗传与种质创新国家重点实验室,农业部国家大豆改良中心 ,南京 ,210095)
Author(s):
Ma Hao -Wang Xiansheng -Liu Chun -Yu Tian -Hao XiaoyanGao Wenrui -He Xiaoling
(State key Laboratory of Crop Genetics and Germplasm Enhancement , National Center for SoybeanImprovement , Ministry of Agriculture, Nanjing Agricultural University , Nanjing , 210095)
关键词:
大豆; 种质资源; SDS - PAGE; 11S 、7S 组分及其亚基; 相对含量
Keywords:
Glycine max (L. ) Merrill ; Germplasm ; SDS - PAGE; 11S ;  7S globulin and theirsubunits ; Relative content
DOI:
10.11861/j.issn.1000-9841.2006.01.0011
文献标志码:
A
摘要:
大豆籽粒贮藏蛋白 7S 和 11S 组分及其亚基含量、11S /7S 比值与大豆蛋白的营养价值和加工特性密切相关 。获得具不同 7S 和 11S 组分及其亚基含量、不同 11S /7S 比值的种质材料是对大豆蛋 白的营养价值和功能特性进行遗传育种改良的重要材料基础 。本研究利用 SDS - PAGE 技术,对 706 份中国大豆种质资源 7S 、11S 组分及其亚基相对含量进行了研究 。结果表明:706 份我国大豆种质资源中 7S 、11S 组分及其亚基相对含量具有丰富的遗传变异;7S 和 11S 组分含量间存在极显著的负相关(r =- 1. 00 ,P <0. 01);603 份地方品种和 103 份新育成品种或主栽品种的 7S、11S 组分相对含量的平均值和变异幅度分别为 40. 00%, 20. 58%～ 56. 65%, 60. 00%, 43. 35%～79. 42%和 38. 21 %,30. 33%～ 52. 67%, 61. 79%, 47. 33%～ 69. 67%;11S /7S 比值的平均值和变异幅度分别为 1. 54,0. 77～ 3. 86 和 1. 65,0. 90～ 2. 30;筛选获得了 63 份 7S 、11S 组分或亚基含量变异种质。
Abstract:
　T he contents of 7S and 11S globulins and their respective subunits, and the 11S /7S ra-tios were tightly associated with the nutrition and processing properties of soybean protein. Forimproving the nutritional qualities and the functional properties of soybean seed proteins, obtai-ning germplasm with different contents of 7S and 11S globulins and their respective subunits anddifferent ratios of the 11S/7S is a desirable objective. 706 accessions from Chinese germplasmwere investigated by Sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS - PAGE).The results showed that 7S globulin was divided as three subunits(α’,α,β), and 11S globulin wasdivided into three bands which were represented as A 3 , A 1 aA 1 bA 2 A 4 (Acid subunits) andB 1 aB 1 bB 2 B 3 B 4 (Basic subunits), respectively. There were great genetic diversity of the relativecontents of 11S , 7S globulins and their subunits in 706 Chinese soybean germplasm. Remarkablynegative correlation between the relative contents of 7S and 11S globulins (r =- 1. 00, P <0. 01)existed. The average and variation range of the relative content of 7S , 11S fraction of 603 Chineselandraces and 103 cultivars were 40. 00%,20. 58%～ 56. 65 %, 60. 00%, 43. 35%～ 79. 42% and38. 21%, 30. 33%～ 52. 67%, 61. 79 %, 47. 33%～ 69. 67%, respectively. The average and varia-tion range of the 11S /7S ratios of 603 Chinese landraces and 103 cultivars were 1. 54, 0. 77～ 3. 86and 1. 65, 0. 90～ 2. 30, respectively. 63 accessions screened from 706 germplasm showed poly-morphism of band type for the subunit relative contents.

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备注/Memo

基金项目:农业部 “948”项目(2001 - 207), 江苏省“十五”重点攻关项目(Q200126)　作者简介:麻 浩(1965- ), 男, 教授, 博士后. 主要从事作物品质改良与加工利用研究. Tel:025- 84395324, E - mail:Lq-ncsi@ njau.edu. cn

更新日期/Last Update: 2016-12-12