[1]龚学臣 、 季 静 抗艳红 王 罡 * 吴 颖 王 　 萍.八氢番茄红素合成酶基因(PSY)对大豆的遗传转化*[J].大豆科学,2005,24(01):30-33.[doi:10.11861/j.issn.1000-9841.2005.01.0030]
　Gong Xuechen Ji Jing Kang Yanhong Wang Gang Wu Ying Wang Ping.GENETIC TRANSFORMATION OF PHYTOENE SYNTHASE GENE(PSY )INTO SOYBEAN[J].Soybean Science,2005,24(01):30-33.[doi:10.11861/j.issn.1000-9841.2005.01.0030]
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八氢番茄红素合成酶基因(PSY)对大豆的遗传转化*

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第24卷 期数: 2005年01期 页码: 30-33 栏目: 出版日期: 2005-02-25
Title:
GENETIC TRANSFORMATION OF PHYTOENE SYNTHASE GENE(PSY )INTO SOYBEAN
作者:
龚学臣 1、2 季 静 1 抗艳红 2 王 罡 1 * 吴 颖 3 王 　 萍 3
(1. 天津大学农业与生物工程学院,天津 300072;2. 河北北方学院农业科学系 ,张家口 075131;3. 淮海工学院海洋学院 ,连运港 222005)
Author(s):
Gong Xuechen 1; 2 Ji Jing 1 　 Kang Yanhong 2 　 Wang Gang 1 　 Wu Ying 3 　 Wang Ping 3
(1. TianjinUniversity , Tianjin , 300072;2. Hebei North University , Zhangjiakou, 075131;3. Huaihai Institute of Technology , Marine School , Lianyungang 222005)
关键词:
PSY 基因; 大豆; 子叶节; 遗传转化
Keywords:
PSY gene; Soybean ; Cotyledonary node; The Genetic transformation
DOI:
10.11861/j.issn.1000-9841.2005.01.0030
文献标志码:
A
摘要:
以三个大豆品种的无菌苗子叶节为受体, 以 PSY 为目的基因, 应用根癌农杆菌介导法对大豆进行了遗传转化, 经子叶节丛生芽诱导 、茎伸长培养 、生根培养和潮霉素选择培养 ,获得了完整的抗性再生苗 ,经 PCR和 PCR- Southern 分析鉴定 ,初步证明外源基因 PSY 已整合到大豆的基因组中 ,并对影响遗传转化的因子进行了研究。
Abstract:
T aking cotyledonary nodes of three varieties of soybean as acceptor , PSY gene were transformedinto soybean via agrobacterium - mediated. Hygromycin resistant regeneration plants of soybean were ob-tained by cotyledonary nodes inducting , striking root cultivation and stem elongation cultivation and hy-gromycin screening in selective condition. PCR and PCR - Southern analysis from the transferred geneplants proved that PSY has been transferred into genome of soybean. The factors affecting genetic trans-formation were also investigated.

参考文献/References:

1　 徐昌杰, 张上隆. 柑橘类胡萝卜素合成关键基因研究进展[ J]. 园艺学报, 2002, 29(增刊):619 - 623.

2　 陈选阳, 郑金贵, 袁照年.类胡萝卜素生物合成代谢工程研究进展[ J]. 福建农林大学学报(自然科学版), 2003, 32(3):348 - 352.
3　 Shewmaker C K , Sheehy J A , Daley M . Seed - specific over ex-pression of phytoene synthase:increase in carotenoids and othermetabolic effects[ J] . Plant Journal, 1999, 20(4):401- 412.
4　 韩雅珊. 类胡萝卜素的功能研究进展[ J] . 中国农业大学学报,1999, 4(1):5- 9.
5　 王关林, 方宏筠主编. 植物基因工程原理与技术[ M] . 北京:科学出版社, 2002, 386- 389.

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备注/Memo

项目来源:“国家植物转基因技术研究开发与中试基地建设”专题课题(项目编号 199- B001)

作者简介:龚学臣(1963 -), 男, 硕士, 副教授, 主要从事植物基因工程方面的研究。E. mail:gxc 2004@y eah. net
?通讯作者:王罡, E - mail:wgtt2003@yahoo. com. cn

更新日期/Last Update: 2016-12-23