[1]宁海龙金双义王继安孙德生李文滨洛育李修平鲁振明朱秀清孙树坤.大豆子粒中异黄酮含量的快速测定[J].大豆科学,2004,23(01):45-49.[doi:10.11861/j.issn.1000-9841.2004.01.0045]
　Ning HailongWang JianSun DeshengLi WenbinJin ShuangyiLuo YuLi XiupingLu ZhenmingZhu XiuqingSun Shukun.A FAST METHOD FOR DETERMININGISOFLAVONE CONTENT IN SOYBEAN SEED[J].Soybean Science,2004,23(01):45-49.[doi:10.11861/j.issn.1000-9841.2004.01.0045]
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大豆子粒中异黄酮含量的快速测定

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第23卷 期数: 2004年01期 页码: 45-49 栏目: 出版日期: 2004-02-25
Title:
A FAST METHOD FOR DETERMININGISOFLAVONE CONTENT IN SOYBEAN SEED
文章编号:
1000 -9841(2004)01 -0045 -05
作者:
宁海龙1金双义2王继安1孙德生1李文滨1洛育1李修平1鲁振明3朱秀清3孙树坤3
1 .东北农业大学大豆研究所, 哈尔滨150030 ;
2 .黑龙江省庆安县农业科学研究所, 庆安152400 ;
3 .国家大豆工程中心, 哈尔滨150050
Author(s):
Ning Hailong1Wang Jian1Sun Desheng1Li Wenbin1Jin Shuangyi2Luo Yu1Li Xiuping1Lu Zhenming3Zhu Xiuqing3Sun Shukun3
1 .Soybean Research Institute , Northeast Agricultural Universi ty , Harbin 150030;
2 .Qing -an AgronomyScience Research Institute , Qing -an 152400 ;
3 .National Soybean Engineering Center , Harbin 150050
关键词:
大豆; 异黄酮含量; 测定; 种质资源
Keywords:
Soybean ; Isoflavone content ; Determination ; Varieties source
分类号:
S 565.1
DOI:
10.11861/j.issn.1000-9841.2004.01.0045
文献标志码:
A
摘要:
建立了一个快速测定大豆异黄酮含量的方法, 并用该法测定了来自东北三省的50 份大豆材料子粒中的异黄酮含量。该体系的最佳提取条件为:不脱脂, 80 %的乙醇浓度, 不小于20 :1 的物料比(溶剂:原料), 浸提1 小时, 浸提温度不超过50 ℃。东北三省的50 份大豆材料异黄酮含量的平均数为0 .285 %, 品种间变异幅度为0 .194 %-0 .408 %, 主要分布于0 .235 %到0 .295 %之间, 其品种数占全部品种数的56 %。
Abstract:
A fast method for determining isoflavone content in soybean seed w as established and 50 soybean v arietiesfrom northeast China were measured by the new method .The optimum condition of the system include80 % of alcohol density , no less than 20 :1 ratio of solute to impregnant , 1hr of time , no more than 50 ℃.Byanaly sis of the isoflavone content of 50 varieties, the average of the isoflavone content w as 0 .285 %, the variancerange f rom 0 .194 % to 0 .408 %, 56 % of all varieties dist ributed f rom 0 .235 % to 0 .295 %.

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备注/Memo

作者简介:宁海龙(1975 -), 男, 讲师, 主要从事大豆遗传育种及生理生态教学研究。

更新日期/Last Update: 2016-12-31