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[1]喻德跃魏国兰孙英Teemu H Teeri.花器发育调节基因gaga1 转化大豆的初步研究[J].大豆科学,2003,22(02):79-82.[doi:10.11861/j.issn.1000-9841.2003.02.0079]
　Yu DeyueWei GuolanSun YingTeemu H Teeri.TRANSFORMATION OF SOYBEANWITH FLORAL REGULATORY GENE GAGA1[J].Soybean Science,2003,22(02):79-82.[doi:10.11861/j.issn.1000-9841.2003.02.0079]

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花器发育调节基因gaga1 转化大豆的初步研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第22卷期数: 2003年02期页码: 79-82 栏目: 出版日期: 2003-05-25

Title:: TRANSFORMATION OF SOYBEANWITH FLORAL REGULATORY GENE GAGA1

文章编号:: 1000 -9841(2003)02 -0079 -04

作者:: 喻德跃¹魏国兰¹孙英¹Teemu H Teeri²; 1.南京农业大学大豆研究所, 国家大豆改良中心, 作物遗传与种质创新国家重点实验室, 南京210095 ;
2.Institute of Biotechnology , 00014 -University of Helsinki , Finland

Author(s):: Yu Deyue¹Wei Guolan¹Sun Ying¹Teemu H Teeri²; Nanjing Agricultural University , Soybean Research Insti tute , National Center for SoybeanImprovement , National Key Laboratory of Crop Genetics and Germplasm Enhancement ,Nanjing 210095 , Inst itute of Biotechnology , 00014 -University of Helsink i ,Finland

关键词:: 发育调节基因; 遗传转化; 花粉管通道; 大豆

Keywords:: Regulato ry genes ; Genetic transformat ion ; Pollen tube pathw ay ; Soybean

分类号:: S 565.103.53

DOI:: 10.11861/j.issn.1000-9841.2003.02.0079

文献标志码:: A

摘要:: 通过花粉管通道法用非洲菊基因gaga1 转化N2899(质核互作雄性不育保持系)、通州豆、95 -7等大豆受体。处理的花朵总数为4300 朵, 共获3000 多粒种子。经卡那霉素田间鉴定, 获得了14 株抗卡那霉素的幼苗。通过抗性标记基因npt Ⅱ和目标基因gaga1 的PCR 检测, 发现有两株N2899 具有阳性信号, 初步证明非洲菊基因gaga1 已整合到这两株大豆的基因组中。形态分析表明:与未转化N2899 相比, 转基因大豆株型矮小, 始花期提前, 盛花期花数量较少, 结荚不多。显微分析发现, 与对照相比转基因大豆花器官结构没有明显改变。

Abstract:: Transformation of soybean w ith gaga1 was conducted using several accessions includingN2899 ,Tongzhoudou , 95 -7etc .through pollen tube pathw ay .About 3000 seeds w ere obtained from 4300 flowerstreated w ith Ti plasmid pHT T613 .By indirect identification method w ith Kanamycin in the field , 14 plant s obtainedw ere found resistant to Kanamy cin .These 14 plants were examined by PCR w ith NPT Ⅱ and gaga 1 specificprimers .Tw o N2899 plant show ed positive signals .This indicated preliminarily that gaga1 w as integratedinto the genome of the tw o plants .These tw o t ransgenic plants were shorter and had less flowers during fullblooming period , but no obvious modification was found in f lo ral st ructure compared to unt ransformed N2899 .The detailed characterization of t ransgenic plants shall be carried out in further generations .

参考文献/References:

1　王关林, 方宏筠.植物基因工程原理与技术[ M] .北京:科学出版社, 1998.

2 　孙敬.利用卡那霉素间接鉴定法进行大规模的棉花转基因育种技术[ J] .作物学报, 2000 , 12(5):270 -276 .

3　魏国兰.非洲菊花器发育调节基因gaga1 转化大豆的初步研究[ D] .南京农业大学硕士论文, 2001 .

4 　Coen ES , Meyerow itz EM .The w ar of the w horls :genetic int eractions controlling flow er developmen t.Nature, 1991 , 353 :31 -37 .

5　Kotilainen M .GRCD1 , an AGL2 -like MADS box gene, participates in the C funct ion du ring s tamen development in Gerbera hybrida[J] .Plant Cell , 2000 , 12 :1893 -1902.

6　S ambrook J .Molecular cloning :A laboratory manual (Cold SpringHarbor[ R] .NY;Cold Spring Harbor Laborat ory Press), 1989 .

7　Thei ssen G.C lassi fication and phylogeny of the MADS -box genemult igene fami ly suggest defined roles of MADS -box gene subfamiliesin the morphological evelu tion of eukaryotes [ J ] .Journal ofMolecular Evaluat ion , 1996 , 43 :484 -516 .

8 　Weigel D, Meyerow itz EM .The ABCs of floral homeoti c genes[ J] .Cell , 1994 , 78 :203 -209 .

9 　Yu DY .Organ identit y genes and modified pat terns of flow er developmentin Gerbera hybrida (Asteraceae)[ J] .Plant Jou rnal, 1999 ,17(1):51 -62 .

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备注/Memo

基金项目:国家转基因植物研究与产业化专项(J00 -B -003 -07)、教育部霍英东青年教师基金(71024)和江苏省青年科技基金(BQ98033)

作者简介:喻德跃(1965 -), 男, 教授, 博士生导师, 研究方向植物分子遗传学与生物技术。E .mail:dyyu @smtp .njau .edu .cn

更新日期/Last Update: 2016-12-31