[1]杨晓英　刘友良 　罗庆云　刘兆普.盐胁迫下野生大豆叶片中Na + 、Cl -积累导致活性氧伤害[J].大豆科学,2003,22(02):83-87.[doi:10.11861/j.issn.1000-9841.2003.02.0083]
　Yang Xiaoying Liu Youliang Luo Qingyun Liu Zhaopu.ACCUMULATION OF Na+ 、Cl-IN THE LEAVES OF WILD SOYBEANSEEDLINGS LEAD TO THEOXIDATIVE DAMAGE UNDER SALT STRESS[J].Soybean Science,2003,22(02):83-87.[doi:10.11861/j.issn.1000-9841.2003.02.0083]
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盐胁迫下野生大豆叶片中Na + 、Cl -积累导致活性氧伤害

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第22卷 期数: 2003年02期 页码: 83-87 栏目: 出版日期: 2003-05-25
Title:
ACCUMULATION OF Na+ 、Cl-IN THE LEAVES OF WILD SOYBEANSEEDLINGS LEAD TO THEOXIDATIVE DAMAGE UNDER SALT STRESS
文章编号:
1000 -9841(2003)02 -0083 -05
作者:
杨晓英　刘友良 　罗庆云　刘兆普
南京农业大学生命科学学院, 农业部作物生长调控重点实验室, 南京210095
Author(s):
Yang Xiaoying 　Liu Youliang 　Luo Qingyun 　Liu Zhaopu
Key Laboratory of Crop Regulation , Minist ry of Agriculture , Col lege of Li feScience , Nanjing Agricuture University , Nanjing 210095
关键词:
野生大豆; 耐盐性; 活性氧代谢; 膜结合酶
Keywords:
Wild soybean ; Salt tolerance ; Active oxygen metablism ; Membrane -associated enzyme
分类号:
S 565.101
DOI:
10.11861/j.issn.1000-9841.2003.02.0083
文献标志码:
A
摘要:
以耐盐性强弱不同的野生大豆(JWS 耐盐性强, N23232 耐盐性弱)为材料, 采用砂培试验研究了NaCl 胁迫下野生大豆叶片中Na+ 、Cl-含量及活性氧代谢的变化。结果表明, 盐胁迫下供试两种群野生大豆叶片Na+ 、Cl-含量增加,N23232 比JWS 更明显;耐盐性弱的N23232 叶片O-.2 产生速率和H2O2 含量上升, 膜脂过氧化产物MDA 和电导率迅速上升, 其叶片中Na+ 、Cl-含量变化与O-.2 、H2O2 、MDA 和电导率增加呈极显著或显著正相关, 而O-.2 产生速率和H2O2 含量上升与MDA 和电导率变化呈极显著正相关。表明在NaCl 胁迫下, 由于Na+ 、Cl-在叶片中的积累而导致了活性氧的积累, 活性氧诱发膜脂过氧化、破坏膜结构, 导致膜结合酶H+-ATPase 和H+ -PPase活性降低, 膜透性增加。耐盐种群JWS 在盐胁迫下Na+ 、Cl-积累量少、活性氧水平较低, 对膜系统伤害少, 膜结合酶活性高, 因而能维持液泡膜正常的代谢功能, 可能是其耐盐性强的原因之一。
Abstract:
The dif ferent salt tolerance of w ild soybean (JWS salt -tolerant , N23232 salt -sensitive)w ere usedto study the changes of Na+ 、Cl-contents and active oxygen metablism in the leaves under NaCl stress by themethods of pot experiment .The result s show ed that the Na+ 、Cl-contents increased in the leaves of the tw opopulation w ild soy bean under salt stress and the Na+ 、Cl-contents w as greater in N23232 than that in JWS .For salt -sensi tive w ild soybean(N23232), the O-.2 generation rate and H2O2 content increased , product of lipidperoxidation(MDA)and elect rical conductivity increased in the leaves under 130 mmol/L NaCl stress .Therew ere some significant ly posi tive co rrelation among the Na+ 、Cl- 、O-.2 、H2O2 、MDA and elect rical conductivityrespectively , and also between O-.2 、H2O2 and MDA 、elect rical conductivity in the salt -sensitive w ild soybean(N23232).It suggested that the content of Na+ 、Cl-increased lead to the accumulation of active oxy gen andthe o xidat ive damage .This oxidative damage expressed as the increase of MDA , the decrease of membrane -associatedenzyme(H+-ATPase and H+-PPase)activi ties and the increase of elect rical conductivi ty .How ever ,the Na+ 、Cl-contents in the leaves of salt -tolerant w ild soybean were maintained at a lower level , and nochanges were detected in O-.2 、H2O2 and MDA contents wi th respect to the cont rol .Therefo re , the damage effectsof active oxy gen species w ere lower on the salt -tolerant w ild soybean than that on the salt -sensitive ones,and the membrane -associated enzyme(H+-ATPase and H+ -PPase)activities maintained at a higher level.The results indicated that the high level of H+ -ATPase and H+ -PPase activities in salt -tolerant w ild soybeanmight be one of the factors of salt tolerance .

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备注/Memo

基金项目:863 海洋生物技术项目(2001AA627040)和高等学校博士学科点专项基金(1999002005)资助。

作者简介:杨晓英(1976 -), 女, 硕士, 主要从事植物耐盐生理研究, 现在中国科学院南京土壤研究所工作。

更新日期/Last Update: 2016-12-31