[1]关荣霞　常汝镇　邱丽娟.用于SSR 分析的大豆DNA 的快速提取[J].大豆科学,2003,22(01):73-74.[doi:10.11861/j.issn.1000-9841.2003.01.0073]
　Guan Rongxia Chang Ruzhen Qiu Lijuan.RAPID ISOLATION OF SOYBEAN DNA FOR SSR ANALYSIS[J].Soybean Science,2003,22(01):73-74.[doi:10.11861/j.issn.1000-9841.2003.01.0073]
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用于SSR 分析的大豆DNA 的快速提取

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第22卷 期数: 2003年01期 页码: 73-74 栏目: 出版日期: 2003-02-25
Title:
RAPID ISOLATION OF SOYBEAN DNA FOR SSR ANALYSIS
文章编号:
1000 -9841(2003)01 -0073 -02
作者:
关荣霞　常汝镇　邱丽娟
中国农业科学院作物品种资源研究所100081
Author(s):
Guan Rongxia 　Chang Ruzhen 　Qiu Lijuan
Institute of Crop Germplasm and Resources , CAAS , Beijing 100081
关键词:
大豆; DNA ; SSR
Keywords:
Soybean ; DNA SSR
分类号:
S 565.1
DOI:
10.11861/j.issn.1000-9841.2003.01.0073
文献标志码:
A
摘要:
描述了一种可从大豆种子及叶片中快速提取DNA 的方法, 既节省时间, 又可获得供上百次PCR 扩增使用的DNA 。并通过实验证明该方法获得的DNA 可用于大豆SSR 分析。
Abstract:
Good quality and quantity DNA can be isolated rapidly from soybean seed and leaf sample by this simplemethod , and the DNA was successfully used for SSR analysis .

参考文献/References:

1 　赵洪锟, 王玉民, 李启云, 等.不同纬度栽培大豆和野生大豆SSR分析[ J] .大豆科学,2001 , 20(3):172-176.

2 　Cregan P B .Jarvik T , Shoemaker R C , Lark K G , .et al.An integratedgenetic linkage map of the soybean genome[ J] .Crop Sci.1999 , 39 :1464 -1490.
3 　王永军, 盖钧镒, 邢邯, 等.抗感SCN 基因的一个RAPD 标记[ J] .大豆科学, 2000 , 19(4):293 -298.
4　周思君.小样品大批量大豆模板DNA 快速分离法[ J] .大豆科学,1999 , 18(4):318-321.
5　张永明, 孙彩云, 梁承邺.一步法提取植物DNA 用于大规模RAPD 分析[ J] .遗传, 2000 , 22(2):106

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备注/Memo

作者简介:关荣霞(1974-), 女, 博士, 研究方向大豆分子标记辅助育种。导师:常汝镇研究员。

更新日期/Last Update: 2016-12-31