[1]卜云萍　李明春　胡国武　王广科　邢来君.向大豆导入深黄被孢霉■6 -脂肪酸脱氢酶基因的初步研究[J].大豆科学,2003,22(01):11-17.[doi:10.11861/j.issn.1000-9841.2003.01.0011]
　Bu Yunping Li Mingchun Hu Guowu Wang Guangke Xing Laijun.A PRELIMINARY STUDY ON TRANSFERRING ■6 -FATTY ACID DESATURASEGENE FROM MORTIERALLA ISABELLINA INTO SOYBEANS[J].Soybean Science,2003,22(01):11-17.[doi:10.11861/j.issn.1000-9841.2003.01.0011]
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向大豆导入深黄被孢霉■6 -脂肪酸脱氢酶基因的初步研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第22卷 期数: 2003年01期 页码: 11-17 栏目: 出版日期: 2003-02-25
Title:
A PRELIMINARY STUDY ON TRANSFERRING ■6 -FATTY ACID DESATURASEGENE FROM MORTIERALLA ISABELLINA INTO SOYBEANS
文章编号:
1000 -9841(2003)01 -0011 -06
作者:
卜云萍　李明春　胡国武　王广科　邢来君
南开大学微生物系, 天津300071
Author(s):
Bu Yunping 　Li Mingchun 　Hu Guowu 　Wang Guangke 　Xing Laijun
Department of Microbiology , Nankai University , Tianjin 300071
关键词:
大豆; ■6 - 脂肪酸脱氢酶基因; 农杆菌介导法; 转基因植株
Keywords:
Soybean ; ■6 -fatty acid desaturase gene ; Agrobacterium infection ; Transferred gene plant
分类号:
S 565.1
DOI:
10.11861/j.issn.1000-9841.2003.01.0011
文献标志码:
A
摘要:
通过农杆菌介导法, 将深黄被孢霉■6 - 脂肪酸脱氢酶基因导入到栽培大豆吉育43 、黑农36 、黑农37 等品种。采用多种外植体和感染方法, 从子叶节和胚轴诱导出丛生芽与再生植株。经过在含50mg L 卡那霉素的筛选培养基中连续筛选, 获得一批转基因植株。经PCR 检测和DNA 分子杂交分析, 初步证明外源基因已导入并整合到大豆基因组中。
Abstract:
The ■6 -fatty acid desaturase gene fron Mortitieralla isabellina was transferred into cultivars Jiyu 43 ,Heinong36 , Heinong 37 , et al .via Agrobacterium infection by inducting with various explant methods .Adentitious budsand regeneration plant were inducted from cotylendon node , hypocotyl .Kanamycin resistant (Kmr)plantlets were obtainedby 3 -4 cycles screening in selective condition .PCR and Southern blot analysis from the transferred gene plantsproved that foreign gene were transferred and integrated into soybean genome .

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备注/Memo

基金项目:国家自然科学基金项目(30200176)和天津市自然科学基金重点项目(013802511)

作者简介:卜云萍(1971-), 女, 博士研究生, 从事分子真菌学及转基因植物工作。

更新日期/Last Update: 2016-12-31