[1]刘德璞　袁鹰　王成武　周正平　郑培和.导入外源DNA 大豆后代的抗虫性鉴定与筛选[J].大豆科学,2002,21(04):245-249.[doi:10.11861/j.issn.1000-9841.2002.04.0245]
　Liu Depu Yuan Ying Wang Chengwu Zhou Zhengping Zheng Peihe.SELECTING AND EVALUATION OF MUTANT LINES RESISTANT TO INSECT PESTBY INDRODUCING EXOGENOUS DNA INTO SOYBEAN[J].Soybean Science,2002,21(04):245-249.[doi:10.11861/j.issn.1000-9841.2002.04.0245]
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导入外源DNA 大豆后代的抗虫性鉴定与筛选

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第21卷 期数: 2002年04期 页码: 245-249 栏目: 出版日期: 2002-11-25
Title:
SELECTING AND EVALUATION OF MUTANT LINES RESISTANT TO INSECT PESTBY INDRODUCING EXOGENOUS DNA INTO SOYBEAN
文章编号:
1000 -9841(2002)04 -0245 -05
作者:
刘德璞　袁鹰　王成武　周正平　郑培和
吉林省农业科学院公主岭136100
Author(s):
Liu Depu 　Yuan Ying 　Wang Chengwu 　Zhou Zhengping 　Zheng Peihe
Jilin Academy of Agricultural Sciences , Gongzhuling 136100
关键词:
大豆; DNA 导入; 抗虫育种; 食心虫; 蚜虫
Keywords:
Soybean ; DNA introduction ; Breeding of resistance to insect ; Leguminivora glgcinivorella ; Aphidglycines
分类号:
S 565.103.53
DOI:
10.11861/j.issn.1000-9841.2002.04.0245
文献标志码:
A
摘要:
大豆食心虫(Leguwinivora glycinivorella )和大豆蚜虫(Aphid glycines )是东北大豆生产的主要虫害。为拓宽资源的利用, 创造抗虫大豆种质, 用花粉管通道技术向大豆品种吉20 、吉25 、吉27 、吉30 等导入皂角(Gleditisia japonica )、鹰嘴豆(Cicer arietinum )和农家早期品种DNA , 对从后代中选出的变异系进行了多年抗虫性鉴定和筛选, 得到了抗虫品系4 个。本文报告了抗虫鉴定筛选结果。
Abstract:
Leguminivora glycinivorella and aphid glycines are major insect pest of soybean in northeastern area of China.In order to broaden applying of germplasm resource and create new variety resisting to insect pest , exogenous DNAform Gleditisia japonica , cicer arietinum and land race were introduced into soybean cultivars of Jilin 20 , Jilin 25 , Jilin27 and Jilin 30 by pollen tube pathway technique .Four lines resistant to the insects (leguminivora glycinivorella or aphid glycines )were gained from the mutantsthrough appraising test for 3 -6 years .This paper report the results of selecting and appraising test .

参考文献/References:

1 　曾君祉, 王东江, 吴有强, 等.用花粉管途径获得小麦转基因植株[ J] .中国科学(B), 1993 , 24 (6):596-601.

2 　曾君祉, 吴有强, 王东江, 等.花粉管通道(或运载)法转化的植株后代遗传表现及转化机理的探讨[ J] .科学通报, 1998, 43(6):561-566.
3 　刘德璞, 袁鹰.外源DNA 导入大豆变异后代的SOD 同工酶分析[ J] .大豆科学1991 ,10(3):194 -199.
4　刘德璞, 廖林, 袁鹰, 等.导入外源DNA 获得抗SMV 大豆品系[ J] .大豆科学, 1997 , 16(4):277 -282.
5 　周明祥.作物抗虫性原理及应用[ M] .北京, 北京农业大学出版社, 1992, 329 -337.

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备注/Memo

作者简介:刘德璞(1951-), 男, 副研究员, 主要从事大豆生物技术研究。

更新日期/Last Update: 2016-12-30