[1]马淑英　尹田夫 袁鹰　刘德璞　刘艳芝.盐胁迫对大豆发育子叶愈伤组织的生化影响[J].大豆科学,1997,16(03):227-232.[doi:10.11861/j.issn.1000-9841.1997.03.0227]
　Ma ShuyingYin Tanfu Yua n YingLiu DepuLiu Ya nzhing.EFFECT OF SALT STRESS ON SOYBEAN COTYLEDON CALLUSTISSUE DEVELOPMENT[J].Soybean Science,1997,16(03):227-232.[doi:10.11861/j.issn.1000-9841.1997.03.0227]
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盐胁迫对大豆发育子叶愈伤组织的生化影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第16卷 期数: 1997年03 页码: 227-232 栏目: 出版日期: 1997-08-20
Title:
EFFECT OF SALT STRESS ON SOYBEAN COTYLEDON CALLUSTISSUE DEVELOPMENT
作者:
马淑英　尹田夫 袁鹰　刘德璞　刘艳芝
(中国人民解放军农牧大学, 长春　130062)
(吉林省农科院大豆所,公主岭　136100)
Author(s):
Ma Shuying　Yin Tanfu Yua n Ying　Liu Depu　Liu Ya nzhing
(University of Agricultural and Animal Science of PLA )
( Soybean Research Institute , Ji lin Academy of Agricultural Science)
关键词:
大豆;  愈伤组织; 盐胁迫;  蛋白质;  氨基酸; 可溶性糖
Keywords:
Salt st ress;  Protein;  Amino acid;  Soluble suga r;  Soy bean callus
DOI:
10.11861/j.issn.1000-9841.1997.03.0227
文献标志码:
A
摘要:
在含不同浓度NaCl的M S培养基上,继代5次培养大豆发育子叶的愈伤组织,分析了不同盐浓度培养的大豆愈伤组织的蛋白质、氨基酸和可溶性糖的含量。经测定表明,盐胁迫下,大豆愈伤组织的蛋白质,总氨基酸含量都比对照高,可溶性糖含量比对照低。NaCl浓度在0～ 0. 1%范围内,随NaCl浓度的增加,蛋白质与总氨基酸的含量逐渐增加,当NaCl浓度增至0. 15% 时,蛋白质和总氨基酸含量又降低。在含NaCl 0. 07% 培养基中,大豆愈伤组织的脯氨酸含量最高,为对照的2. 72倍。盐胁迫下、脯氨酸、丙氨酸、精氨酸和组氨酸的含量变化与总氨基酸含量变化不一致。
Abstract:
The y oung co tyledo n soybean ca llus tissues w ere cultured in the di fferent co ncentrationNaCl medium. Fo r fiv e genera tions, the co ntents o f protein, amino and so lublesug ar of soybea n callus ti ssue w ere measured. The resul t show ed tha t co ntent s of proteinand amino o f ca llus ti ssue under salt stress w ere higher tha n those of co ntro l. Solublesug ar content w as low er than tha t of cont ro l. Except in 0. 15% NaCl, the co ntentso f protein and amino were increased wi th increase of NaCl co ncent ratio n. The accumulationo f proline in 0. 07% NaCl medium w as 2. 72 times hig her tha n in co ntro l. Thecha ng es of proline, alanine, arginine a nd histidine w ere dif ferent f rom the chang e of totalamino under sal t st ress.

参考文献/References:

[1 ]　Rath eri, G, J. of Pl an t Nut r. 1985, 8( 3)

[2 ]　陈云昭等, 1992, 在盐胁迫下获得的大豆愈伤组织及再生植株的生化反应,大豆科学, ( 11): 70～ 73
[3 ]　周荣仁等, 1989, 利用组织培养研究植物耐盐机理与筛选耐盐突变体的进展,植物生理学通讯, ( 5): 11～ 19
[4 ]　邵桂花等, 1993, 大豆耐盐性研究进展,大豆科学, 12( 3): 244～ 248
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更新日期/Last Update: 2017-01-13