[1]刘俊,李海洋,贺米兰,等.大豆TOE基因的进化分析及开花调控功能解析[J].大豆科学,2021,40(05):581-591.[doi:10.11861/j.issn.1000-9841.2021.05.0581]
　LIU Jun,LI Hai-yang,HE Mi-lan,et al.Molecular Evolutionary and Flowering Regulation Function Analysis of TOE Genes in Soybean[J].Soybean Science,2021,40(05):581-591.[doi:10.11861/j.issn.1000-9841.2021.05.0581]
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大豆TOE基因的进化分析及开花调控功能解析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第40卷 期数: 2021年05期 页码: 581-591 栏目: 出版日期: 2021-09-20
Title:
Molecular Evolutionary and Flowering Regulation Function Analysis of TOE Genes in Soybean
作者:
刘俊; 李海洋; 贺米兰; 张婷; 刘宝辉; 赵晓晖
（广州大学 生命科学学院，广东 广州 510006）
Author(s):
LIU Jun;  LI Hai-yang;  HE Mi-lan;  ZHANG Ting;  LIU Bao-hui;  ZHAO Xiao-hui
(School of Life Sciences，Guangzhou University，Guangzhou 510006，China)
关键词:
大豆; 开花调控; TOE基因; 生物信息学分析; GmTOE6b; 共线性分析; 单倍型
Keywords:
Soybean;  Flowering regulation;  TOE genes;  Bioinformatics analysis;  GmTOE6b;  Collinearity analysis;  Haplotype
DOI:
10.11861/j.issn.1000-9841.2021.05.0581
文献标志码:
A
摘要:
为了进一步解析大豆中重要植物开花和花器官发育调控转录因子 AP2 的编码基因 TOE 的进化规律及其对开花功能的调控作用，为大豆 TOE 基因的功能解析和大豆纬度适应性研究提供基础，本研究利用生物信息学手段对大豆 TOE 基因进行聚类分析、序列特征分析、染色体区段共线性分析和组织特异性表达分析，预测关键开花基因启动子区段 AP2 结合位点，并验证不同单倍型大豆开花时间。结果显示：从 PlantTFDB 数据库检索到 12 个大豆 TOE 基因， GmTOE6b （ Glyma . 02G087400 ）为新发现的大豆TOE基因。GmTOE6a和GmTOE6b均只有1个AP2结构域，其余大豆TOE基因均有两个AP2结构域。6个大豆TOE基因与拟南芥TOE1基因聚为一类；2个与拟南芥TOE2基因聚为一类；4个与拟南芥TOE3、AP2聚为一类。12个大豆TOE基因都有且仅有1个miR172靶位点，且该靶位点序列与拟南芥TOE的miR172靶位点序列高度一致。染色体区段共线性分析显示，大豆12个TOE基因按起源方式可以分为3类，6个随大豆基因组复制而产生；4个起源于大豆物种形成之前且与拟南芥TOE基因有共同祖先；2个起源于大豆物种形成之前且与拟南芥TOE基因无共同祖先。大豆开花关键基因GmFT2a和GmFT5a启动子序列中均含有多个AP2结合位点，GmTOE4b和GmTOE5b两个基因均可影响大豆的开花时间。研究结果说明大豆中12个TOE基因极有可能均是miR172的靶基因，虽然其编码蛋白的氨基酸组成非常相似，但它们的进化规律和组织特异性表达规律存在不同，它们在进化过程中可能存在功能分化。GmTOE4b和GmTOE5b可能通过结合GmFT2a和GmFT5a启动子上的顺式元件来调控其基因转录，从而调控开花。
Abstract:
In order to analyze the evolution of GmTOEs and its regulation on flowering function in soybean, to provide the basis for functional analysis of GmTOEs and research on latitude adaptability of soybean, we used bioinformatics methods to perform cluster analysis, sequence characterization analysis, chromosomal segment collinearity analysis and tissue specific expression analysis of GmTOEs, predicted AP2 binding sites in the promoter regions of important flowering genes and verified flowering times in different haplotypes of soybean. The results showed that 12 GmTOEs were retrieved from PlantTFDB database, and GmTOE6b (Glyma.02G087400) was a newly discovered TOE gene in soybean. GmTOE6a and GmTOE6b had only one AP2 domain, while the other GmTOEs had two AP2 domains. Six GmTOEs were clustered into the same group as the Arabidopsis TOE1, two were clustered with Arabidopsis TOE2, four were clustered with Arabidopsis TOE3 and AP2. Each GmTOEs had one miR172 target site, and the target site sequence was highly consistent with AtTOEs. Chromosome segment collinearity analysis showed that GmTOEs could be divided into three categories according to their origin, and six of them were generated with the replication of soybean genome. Four of them originated before the speciation of soybean and shared a common ancestor with AtTOEs. Two of them originated before the speciation of soybean and had no common ancestor with AtTOEs. The important flowering regulation genes GmFT2a and GmFT5a have multiple AP2 binding sites in their promoter, and both GmTOE4b and GmTOE5b can affect the flowering time of soybean. The results indicated that the 12 GmTOEs were the most likely to be the target of miR172. Although the amino acid composition of the protein encoded by them was very similar, their evolutionary rules and tissue-specific expression were different, and they may have functional differentiation during the evolutionary process. GmTOE4b and GmTOE5b may regulate the transcription of GmFT2a and GmFT5a by binding to their cis acting elements on the promoters, to regulate flowering.

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备注/Memo

收稿日期：2021-03-09基金项目：国家自然科学基金（31801383）；广东省自然科学基金（2018A030313199）。第一作者：刘俊（1995—），男，硕士，主要从事大豆开花调控分子机理研究。E-mail：2111814034@e.gzhu.edu.cn。通讯作者：赵晓晖（1985—），女，博士，副教授，主要从事大豆分子遗传学研究。E-mail：zhaoxiaohui_1234@163.com。

更新日期/Last Update: 2021-09-27