WANG Min,QIN Jie,YANG Wan-ming,et al.Screening of Salt-Tolerant and Well Symbiotic Matching Soybean Rhizobia Strains for Jinda 88[J].Soybean Science,2021,40(03):385-393.[doi:10.11861/j.issn.1000-9841.2021.03.0385]
晋大88高匹配性强耐盐根瘤菌筛选
- Title:
- Screening of Salt-Tolerant and Well Symbiotic Matching Soybean Rhizobia Strains for Jinda 88
- Keywords:
- Soybean; Shanxi; Salt-tolerance; Rhizobia strains; Screening
- 文献标志码:
- A
- 摘要:
- 为筛选与山西主栽品种晋大88匹配性较好的强耐盐根瘤菌，本研究选用从187株大豆根瘤菌株中筛选出的7株强耐盐性根瘤菌株为供试材料，以USDA110为对照菌株，采用随机区组试验设计，将不同菌株分别接种在清水对照和150 mmol?L-1 NaCl溶液处理的晋大88植株上，比较其对晋大88性状的影响，采用相关分析和聚类分析方法筛选耐盐性强且匹配性好的大豆根瘤菌菌株。结果表明：晋大88的大多数生长性状在NaCl胁迫下较清水对照组有所下降，其中根瘤性状表现出显著或极显著下降。在清水对照下菌株USDA110处理的植株根瘤鲜重、根瘤干重和根瘤数量均较高，与晋大88的匹配性最好。NaCl胁迫下菌株TG1处理的植株叶片SPAD值、根瘤鲜重、根瘤干重、根瘤数量表现最好，菌株TG14处理的植株株高、地上部鲜重表现最好。相关分析表明相对根瘤数与相对SPAD值相关极显著，相对根瘤鲜重与相对根瘤干重相关极显著，而相对地上部鲜重与相对株高、相对地上部干重呈显著相关关系。聚类分析表明菌株TG1、TG53、TG79和TG14处理的植株表现出较好的耐盐性，本研究筛选得到的菌株为山西省大豆耐盐根瘤菌的推广应用提供了菌株资源。
- Abstract:
- In order to screen soybean rhizobium with strong salt-tolerance for Shanxi main cultivar Jinda 88, and provide theoretical basis for solving the application of rhizobia in saline soil, 7 salt-tolerant rhizobia strains screened from 187 soybean rhizobia strains and 1 reference rhizobia strains USDA110 were used for tested strains. All strains were inoculated on Jinda 88 treated with water control and 150 mmol〖DK1〗?L-1 NaCl using randomized block design, in order to analyze the effects of rhizobium on growth traits of Jinda 88 and screen out the salt-tolerant and well-matched rhizobia strains by using correlation analysis and cluster analysis. The results showed that most of the growth traits reduced under salt stress, especially nodule traits were decreased significantly or extremely significantly. The nodule traits including fresh weight and dry weight of root nodules and root nodule number were significantly increased in USDA110 inoculated plants, which indicated that well compatibility of soybean-rhizobia in water control was Jinda 88-USDA. The plant leaf SPAD value, nodule fresh weight, dry weight of root nodules and nodule number were the best for TG1 inoculated plants under salt stress. The plant height and aboveground fresh weight were the best for TG14 inoculated plants under salt stress. Correlation analysis showed that the relative nodule number was significantly correlated with the relative SPAD value, the relative nodule fresh weight was significantly correlated with the relative nodule dry weight, and the relative shoot fresh weight was significantly correlated with the relative plant height and the relative shoot dry weight. Cluster analysis showed that strain TG1, TG53, TG79 and TG14 inoculated plants showed stronger salt resistance. These strains provided theoretical basis for the research on salt tolerance of soybean rhizobia strains and strain resources for the popularization and application in salinity soil of Shanxi Province.
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备注/Memo
收稿日期：2021-01-27