YANG Jin,WANG Xiao-men,YE Wen-wu,et al.Identification of Soybean Resistance to Phytophthora sojae in the Germplasm Resources from Huanghuaihai Region of China[J].Soybean Science,2020,39(01):12-22.[doi:DOI：10.11861/j.issn.1000-9841.2020.01.0012]
黄淮海地区大豆种质资源对疫霉根腐病的抗性鉴定
- Title:
- Identification of Soybean Resistance to Phytophthora sojae in the Germplasm Resources from Huanghuaihai Region of China
- Keywords:
- Root rot; Phytophthora sojae; Soybean germplasm resources; Disease resistance identification; Resistance genes; Huanghuaihai region
- 文献标志码:
- A
- 摘要:
- 为系统鉴定黄淮海地区大豆种质资源对大豆疫霉根腐病的抗性，本研究采用改进的黄化苗下胚轴接种方法，利用8个大豆疫霉根腐病菌株对2017和2018年从黄淮海地区各育种单位收集的381个大豆品种（系）进行抗性鉴定。结果显示：22个品种（系）对超强毒力菌株PsJS2表现抗性，超过90%的品种（系）对弱毒力菌株Ps1、Ps3和Ps5均表现抗性。所有大豆品种（系）对8个菌株共产生36种反应型。将待鉴定品种（系）与鉴别寄主对8个大豆疫霉菌株的反应型进行比较，其中有63个品种（系）可能含有抗病基因Rps1b，36个可能含有抗病基因Rps3a，15个可能含有Rps1d；255个品种（系）的32种反应型与任何已知鉴别寄主的反应型均不一致，表明可能含有新的抗病基因或抗病基因的组合。本研究通过室内快速抗性鉴定，发现河南、河北、北京、安徽、山东、山西等地均含有丰富的大豆抗病种质资源，且具有丰富的抗性多样性。其中，河南、山东等地含有丰富的含有抗病基因Rps1b的抗性资源，河北、北京等地含有丰富的含有抗病基因Rps3a的抗病资源，为抗大豆疫霉根腐病的大豆种质利用及抗病基因挖掘提供了重要参考。
- Abstract:
- To identify the resistance level of the soybean cultivars(lines) from Huanghuaihai region to Phytophthora sojae (P. sojae) systematically, we used 381 soybean cultivars (lines) collected in 2017 and 2018 from various soybean breeding institutes, 8 representative P. sojae isolates, and a modified etiolated hypocotyl inoculation method. We found that 22 cultivars (lines) showed resistance to the strong virulent strain PsJS2, and more than 90% cultivars (lines) were resistant to the weak virulent strains Ps1, Ps3 and Ps5. All the cultivars (lines) exhibited 36 resistant/susceptible reaction patterns. The reaction patterns of target cultivars (lines) were compared with the differential lines to the 8 isolates. The reaction pattern occurring in 63 cultivars (lines) was consistent with that in the differential line carrying Rps1b, 36 cultivars (lines) produced reaction pattern coincide with Rps3a and 15 were postulated that contained Rps1d. The other 32 reaction patterns produced by 255 cultivars (lines), not consistent with any known differential line, may contain new Rps gene(s). Therefore, we found abundant resistant cultivars (lines) and diversity in Henan, Hebei, Beijing, Anhui, Shandong and Shanxi used a quickly method in greenhouse. Henan and Shandong possess abundant soybean cultivars (lines) containing Rps1b, Hebei and Beijing possess abundant soybean cultivars (lines) containing Rps3a. The data provided useful information for germplasm sources deployment during soybean breeding.
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备注/Memo
收稿日期：2019-06-19