[1]刘东波,邢国杰,赵倩倩,等.抗病毒转基因大豆事件外源T-DNA序列分析及定性检测[J].大豆科学,2020,39(01):23-29.[doi:10.11861/j.issn.1000-9841.2020.01.0023]
 LIU Dong-bo,XING Guo-jie,ZHAO Qian-qian,et al.Efficient Identification of T-DNA Insertions and Qualitative Analysis in Two Virus-resistant Transgenic Soybean Events[J].Soybean Science,2020,39(01):23-29.[doi:10.11861/j.issn.1000-9841.2020.01.0023]
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抗病毒转基因大豆事件外源T-DNA序列分析及定性检测

参考文献/References:

[1]Latham J R, Wilson A K, Steinbrecher R A. The mutational consequences of plant transformation[J]. Journal of Biomedicine and Biotechnollogy, 2006(2): 25376.[2] Nacry P, Camilleri C, Courtial B, et al. Major chromosomal rearrangements induced by T-DNA transformation in Arabidopsis[J]. Genetics, 1998, 149(2): 641-650.[3] Wilson A K, Latham J R, Steinbrecher R A. Transformation-induced mutations in transgenic plants: Analysis and biosafety implications[J]. Biotechnolnoly and Genetic Engineering Reviews, 2006, 23(1):209-238.[4] Windels P, Sylvie D B, Erik V B, et al. T-DNA integration in Arabidopsis chromosomes. Presence and origin of filler DNA sequences[J]. Plant Physiology, 2003, 133(4): 2061-2068.[5] Codex Alimentarius Commission. Guide line for the conduct of food safety assessment of foods derived from recombinant-DNA plants[S]. FAO/WHO Joint Publications, 2003:1-14. 〖HJ1.5mm〗[6] European Food Safety Authority Panel on Genetically Modified Organisms. Guidance on the environmental risk assessment of genetically modified plants[J]. European Food Safety Authority Journal,2010, 8(11):1879. [7] Kok E J, Pedersen J, Onori R, et al. Plants with stacked genetically modified events: To assess or not to assess[J]. Trends Biotechnology, 2014, 32(2): 70-73. [8] Ochman H, Gerber A S, Hartl D L. Genetic applications of an inverse polymerase chain reaction[J]. Genetics, 1998, 120(3): 621-623.[9] Liu Y G, Mitsukawa N, Oosumi T, et al. Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR[J]. Plant Journal, 8(3):457-463. [10]O′Malley R C, Ecker J R. Linking genotype to phenotype using the Arabidopsis unimutant collection[J]. Plant Journal, 61(6): 928-940.[11]Ji J B, Braam J. Restriction site extension PCR: A novel method for high-throughput characterization of tagged DNA fragments and genome walking[J]. PLoS ONE, 2010, 5: e10577.[12]Spalinskas R, Bulcke M V D, Eede G V D, et al. LT-RADE: An efficient user-friendly genome walking method applied to the molecular characterization of the insertion site of genetically modified maize MON810 and rice LLRICE62[J]. Food Analytical Method, 2013, 6(2): 705-713.[13]Babekova R, Funk T, Pecoraro S, et al. Development of an event-specific real-time PCR detection method for the transgenic Bt rice line KMD1[J]. European Food Research and Technology, 2009, 228(5): 707-716.[14]Milcamps A, Rabe S, Cade R, et al. Validity assessment of the detection method of maize event Bt10 through investigation of its molecular structure[J]. Journal of Agricural and Food Chemistry, 2009, 57(8):3156-3163. [15]Wang J, Wang W, Li R, et al. The diploid genome sequence of an Asian individual[J]. Nature, 2008, 456(7218): 60-65.[16]Ajay S S, Parker S C, Abaan H O, et al. Accurate and comprehensive sequencing of personal genomes[J]. Genome Research, 2011, 21(9): 1498-1505. [17]Lepage E, Zampini E, Boyle B, et al. Time and cost-efficient identification of T-DNA insertion sites through targeted genomic sequencing[J]. PLoS One, 2013, 8(8): e70912.[18]Inagaki S, Henry I M, Lieberman M C, et al. High-throughput analysis of T-DNA location and structure using sequence capture[J]. Plos One, 2015, 10(10): e0139672.[19]Wahler D, Schauser L, Bendiek J, et al. Next-generation sequencing as a tool for detailed molecular characterisation of genomic insertions and flanking regions in genetically modified plants: A pilot study using a rice event unauthorised in the EU[J]. Food Analytical Methods, 2013, 6(6): 1718-1727.[20]Park D, Kim D, Jang G, et al. Efficiency to discovery transgenic loci in GM rice using next generation sequencing whole genome re-sequencing[J]. Genomics & Informatics, 2015, 13(3):81. [21]Williams-Carrier R, Stiffler N, Belcher S, et al. Use of illumina sequencing to identify transposon insertions underlying mutant phenotypes in high-copy mutator lines of maize[J]. The Plant Journal, 2010, 63(1):167.[22]Kovalic D. The use of next generation sequencing and junction sequence analysis bioinformatics to achieve molecular characterization of crops improved through modern biotechnology[J]. Plant Genome, 2012, 5(3):149-163.[23]Guo B, Yong G, Hong H, et al. Identification of genomic insertion and flanking sequence of G2-EPSPS and GAT transgenes in soybean using whole genomes equencing method[J]. Frontiers in Plant Science, 2016, 7: 1009.[24]Yang X D, Niu L, Zhang W, et al. RNAi-mediated SMV P3 cistron silencing confers significantly enhanced resistance to multiple Potyvirus strains and isolates in transgenic soybean[J]. Plant Cell Reports, 2018, 37(1): 103-114.[25]Porebski S, Bailey L G, Baum B R. Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components[J]. Plant Molecular Biology Reporter, 1997, 15(1): 8-15.[26]马阔, 仲晓芳, 牛陆, 等. 耐盐转基因大豆FA8015旁侧序列分离及定性PCR检测[J]. 大豆科学, 2018, 37(6):854-859.(Ma K, Zhong X F, NIU L, et al. Sequence separation and qualitative PCR detection of salt-tolerant transgenic soybean FA8015[J]. Soybean Science, 2018, 37(6): 854-859.)[27]仲晓芳, 杨静, 贺红利, 等. 基于基因组重测序的高含量油酸转基因大豆T-DNA旁侧序列分析及事件特异性PCR检测[J]. 农业生物技术学报, 2018, 26(12):2017-2026.(Zhong X F, Yang J, He H L, et al. Sequence analysis and event specific PCR detection of high content oleic acid transgenic soybean T-DNA based on genomic resequencing[J]. Journal of Agricultural Biotechnology, 2018, 26(12): 2017-2026.)[28]Langmead B and alzberg S L. Fast gapped-read alignment with Bowtie 2[J]. Nature Methods, 2012, 9:357-359.[29]Yang L T, Wang C M, Jensen A H, et al. Characterization of GM events by insert knowledge adapted re-sequencing approaches[J]. Scientific Reports, 2013, 3:2839. [30]Campbell P J, Stephens P J, Pleasance E D, et al. Identification of somatically acquired rearrangements in cancer using genome-wide massively parallel paired-end sequencing[J]. Nature Genetics, 40(6):722-729. [31]Hormozdiari F, Hajirasouliha I, Mcpherson A, et al. Simultaneous structural variation discovery among multiple paired-end sequenced genomes[J]. Genome Research, 2011, 21(12):2203-2212. [32]DuBose A J, Lichtenstein S T, Narisu N, et al. Use of microarray hybrid capture and next-generation sequencing to identify the anatomy of a transgene[J]. Nucleic Acids Research, 2013, 41(6):e70. [33]Kovalic D, Garnaat C, Guo L, et al. The use of next generation sequencing and junction sequence analysis bioinformatics to achieve molecular characterization of crops improved through modern biotechnology[J]. The Plant Genome, 2012, 5(3): 149-163.[34]Urbanski D F, Malolepszy A, Stougaard J, et al. Genome wide LORE1 retrotransposon mutagenesis and high-throughput insertion detection in Lotus japonicus[J]. The Plant Journal, 2012, 69(4):731-741.[35]Daniela W, Leif S, Joachim B, et al.Next-generation sequencing as a tool for detailed molecular characterization of genomic insertions and flanking regions in genetically modified plants: A pilot study using a rice event unauthorized in the EU[J]. Food Analytical Method, 2013, 6(6):1718-1727. [36]Pauwels K, DeKeersmaecker S C J, DeSchrijver A, et al.Next-generation sequencing as a tool for the molecular characterization and risk assessment of genetically modified plants: Add value or not?[J]. Trends in Food Science and Technology, 2015, 45(2):319-326.

相似文献/References:

[1]马阔,仲晓芳,牛陆,等.耐盐转基因大豆事件FA8015旁侧序列分离及定性PCR检测 [J].大豆科学,2018,37(06):854.[doi:1011861/jissn1000-98412018060854]
 MA Kuo,ZHONG Xiao-fang,NIU Lu,et al.Flanking Sequence Isolation of Salt-Tolerant Transgenic Event FA8015 of Soybean and Quantitative PCR Test [J].Soybean Science,2018,37(01):854.[doi:1011861/jissn1000-98412018060854]
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备注/Memo

?收稿日期:2019-06-19

基金项目:国家转基因生物新品种培育科技重大专项(2016ZX08004-004);国家自然科学基金(31701448)。
第一作者简介:刘东波(1993-),男,硕士,主要从事大豆生物技术育种研究。E-mail:87144326@qq.com。
通讯作者:仲晓芳(1978-),女,博士,副研究员,主要从事大豆生物技术育种研究。E-mail:xfzhong649@163.com。

更新日期/Last Update: 2020-03-17