[1]白玉翠,王萍,倪志勇,等.大豆GmNF-YA11基因克隆及特征分析[J].大豆科学,2019,38(05):719-725.[doi:10.11861/j.issn.1000-9841.2019.05.0719]
　BAI Yu-cui,WANG Ping,NI Zhi-yong,et al.Cloning and Characterization of GmNF-YA11 Gene in Soybean[J].Soybean Science,2019,38(05):719-725.[doi:10.11861/j.issn.1000-9841.2019.05.0719]
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大豆GmNF-YA11基因克隆及特征分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第38卷 期数: 2019年05期 页码: 719-725 栏目: 出版日期: 2019-09-20
Title:
Cloning and Characterization of GmNF-YA11 Gene in Soybean
作者:
（新疆农业大学 农学院，新疆 乌鲁木齐 830052）
Author(s):
BAI Yu-cui;  WANG Ping;  NI Zhi-yong;  YU Yue-hua
(College of Agriculture, Xinjiang Agricultural University, Urumqi 830052, China)
关键词:
大豆; GmNF-YA11; 克隆; 转录激活
Keywords:
Soybean;  GmNF-YA11;  Cloning;  Transcriptional activation
DOI:
10.11861/j.issn.1000-9841.2019.05.0719
文献标志码:
A
摘要:
NF-Y类转录因子是真核生物中普遍存在的一种转录因子复合物，在植物胚胎发育、光合作用、开花时间调控、逆境胁迫响应等诸多方面起重要作用，因此研究NF-Y类转录因子对提高大豆抗逆性有重要意义。为初步了解大豆GmNF-YA11基因的基本特征并为后续实验提供理论基础，本研究从大豆中克隆GmNF-YA11基因，其cDNA序列为1 553 bp，包含1个912 bp的开放阅读框，编码1个含303个氨基酸的蛋白质，分子量为 34.06 kD，等电点为9.50；GmNF-YA11基因组包含5个外显子和4个内含子。进化树分析发现GmNF-YA11和GmNF-YA13在同一个分支上。转录活性分析表明，GmNF-YA11转录因子并不具有转录激活功能。组织特异性表达模式分析表明，GmNF-YA11在检测的所有组织中均有表达，在花中的表达量最低，而在种子中的表达量最高。
Abstract:
The NF-Y transcription factor is a ubiquitous transcription factor complex in eukaryotes, recent studies have found that NF-Y plays an important role in plant embryo development, photosynthesis, flowering time regulation, stress response and so on. Therefore, studying NF-Y transcription factors is important to improve soybean resistance. We cloned the GmNF-YA11 gene from soybean in order to preliminarily understand its basic characteristics. The full-length cDNA sequence is 1 553 bp containing open reading frame of 912 bp, encodes a protein of 303 amino acids with a molecular weight of 34.06 kD and pI 9.50. The GmNF-YA11 genome contains five exons and four introns. Phylogenetic tree analysis showed that GmNF-YA11 and GmNF-YA13 were on the same branch. Transcriptional activity analysis showed that GmNF-YA11 transcription factor didn’t have transcriptional activation function. The analysis of tissue specific expression pattern showed that GmNF-YA11 was expressed in all the tissues detected, the highest expression in the seeds and the lowest expression in the flowers.

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备注/Memo

收稿日期：2019-03-22

基金项目：国家自然科学基金（31860295，31660295）；新疆维吾尔自治区天山英才计划（201720085）；新疆农业大学作物学重点学科（XNCDKY2018013）。
第一作者简介：白玉翠（1995-），女，硕士，主要从事植物逆境分子生物学研究。E-mail:1491218269@qq.com。
通讯作者：于月华（1981-），女，高级实验师，博士，主要从事作物分子育种研究。E-mail:yuyuehua1213@sina.com。

更新日期/Last Update: 2019-09-20