ZHAO Xian-wei,ZHAO Chao-sen,WANG Rui-zhen,et al.Analysis of 7S and 11S Globulin and Their Subunits Relative Content of Soybean Germplasm in Jiangxi Province[J].Soybean Science,2019,38(04):503-510.[doi:DOI:10.11861/j.issn.1000-9841.2019.04.0503]
江西省大豆种质资源7S和11S球蛋白及其亚基相对含量分析
- Title:
- Analysis of 7S and 11S Globulin and Their Subunits Relative Content of Soybean Germplasm in Jiangxi Province
- Keywords:
- Soybean; Germplasm; 7S globulin; l1S globulin; Subunit
- 文献标志码:
- A
- 摘要:
- 为明确江西省大豆球蛋白各亚基相对含量及其比例,促进营养或加工品质优良大豆资源的挖掘与应用。本研究采用SDS-PAGE方法结合Gel-Pro Analyzer 4.5软件对供试的131份江西省大豆种质资源球蛋白及其亚基相对含量进行分析。结果表明:江西省大豆资源的7S、11S球蛋白及其亚基相对含量具有丰富的遗传变异,供试材料7S和11S球蛋白相对含量的变幅、平均值及变异系数分别为18.01%~52.21%、32.64%、19.46%和47.79%~81.99%、67.35%、9.43%,11S/7S比值范围为0.92~4.55,平均值为2.19,变异系数为30.64%,且筛选鉴定出3个11S/7S比值大于4.0和4个α′亚基、α亚基或β亚基含量较低的优异大豆种质资源,这些资源的挖掘为大豆品质的改良提供了材料和参考。
- Abstract:
- In order to clarify the relative content variation of globulin and their subunits, and then screening excellent germplasm to improve the nutritional and processing quality of soybean. This study analyzed 7S and 11S globulin and subunits content of 131 soybean accessions originated from Jiangxi province by SDS-PAGE(sodium dodecyl sulfate-polyacrylamide gel electrophoresis). The study showed that the variation range, average and variable coefficient of relative content of 7S and 11S globulins were 18.01%-52.21%, 32.64%, 19.46% and 47.79%-81.99%, 67.35% and 9.43%, respectively. The variation of 11S/7S ration ranged from 0.92 to 4.55, and the average and variable coefficient of 11S/7S were 2.19 and 30.64%, respectively. The gathered data implied that soybean germplasm of Jiangxi province contains abundant genetic diversity of relative contents of 7S, 11S globulin and their subunits, among which 11S/7S ratios of 3 germplasm were higher than 4.0, and 4 accessions showed relative lower contents ofα′, αandβsubunits than others. This study provides a useful reference to improve high-quality breeding of soybean.
参考文献/References:
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