ZHENG Hao-yu,HUANG Bing-lin,WANG Meng-xue,et al.The Effect of Nitrogen Fertilizer Reduction and Rhizobium Inoculation on Soybean Photosynthesis and Yield[J].Soybean Science,2019,38(03):413-420.[doi:10.11861/j.issn.1000-9841.2019.03.0413]
氮肥减施与接种根瘤菌对大豆光合与产量的影响
- Title:
- The Effect of Nitrogen Fertilizer Reduction and Rhizobium Inoculation on Soybean Photosynthesis and Yield
- Keywords:
- Soybean; Nitrogen fertilizer reduction; Rhizobia; Photosynthesis; Yield; Dry matter
- 文献标志码:
- A
- 摘要:
- 为揭示氮肥减施与接种不同根瘤菌对大豆光合作用及产量的影响,通过设置不同减氮量+接种根瘤菌处理,研究其对大豆净光合速率、蒸腾速率、气孔导度、胞间CO2浓度及大豆产量的影响。结果表明:在开花期, T1(1/2施氮+拌种NF)、T2(1/2施氮+土壤施用NF)、T3(1/2施氮+拌种DF)处理的净光合速率相对于CK分别提高了50.4%、30.1%、29.6%。所有处理较常规施肥(CK)的蒸腾速率、胞间CO2浓度、气孔导度均有所上升,而大豆叶绿素含量较CK差异不显著,各处理的叶片干重较CK差异不显著,除T3处理外,各处理的茎干重较CK差异也不显著。进入结荚期后,T1、T2处理的胞间CO2浓度较CK分别提高了34.3%、47.0%,各处理净光合速率较CK均下降,而蒸腾速率、气孔导度均高于CK,除T4、T5外,叶绿素含量较CK差异不显著。除T2处理外,各处理的叶干重均低于CK,茎与荚皮干重较CK差异不显著,籽粒干重在所有减1/2施氮量+接种根瘤菌处理中均显著高于CK。在鼓粒期,各处理的净光合速率、蒸腾速率、胞间CO2浓度较CK差异不显著,气孔导度均高于CK,不施氮肥处理的叶绿素含量均显著低于CK。各处理的叶片干重均显著低于CK,但茎、荚皮干重与CK相比差异不显著,且籽粒干重有一定升高趋势,T3处理籽粒干重较CK提高了24.6%;在成熟期,1/2减氮+接种根瘤菌处理基本上均能提高大豆的产量及其构成因子,尤其是T3处理较CK产量提高9.75%。
- Abstract:
- In order to revealed the effects of nitrogen fertilizer reduction and different of rhizobia inoculation on soybean photosynthesis and yield, the effects of nitrogen fertilizer reduction and rhizobia inoculation on net photosynthetic rate, transpiration rate, stomatal conductance, intercellular CO2 concentration and soybean yield were studied by setting different nitrogen reduction and rhizobia inoculation treatments. Results showed that: In the flowering period, T1 (1/2 N + seed mixing NF), T2 (1/2 N + soil application of NF) and T3 (1/2 N + seed mixing DF) processing of net photosynthetic rate compared with the CK increased by 50.4%, 30.1% and 29.6% respectively, the transpiration rate, intercellular CO2 concentration and stomatal conductance of all treatments increased compared with conventional fertilization (CK), while the chlorophyll content of soybean was not significantly different from CK, dry weight of leaves in each treatment was not significantly different from that of CK,except for T3 treatment, the stem weight of each treatment was not significantly different from that of CK. After entered the podding stage, the intercellular CO2 concentration of T1 and T2 treatment was 34.3% and 47% higher than that of CK respectively, the net photosynthetic rate of each treatment decreased, while the transpiration rate and stomatal conductance were higher than CK, except T4 and T5, the chlorophyll content was not significantly different from that of CK, except T2 treatment, the dry weight of each treatment was lower than that of CK, and the dry weight of stem and pod was higher than CK, different treatment was not significant, dry weight of seed was significantly higher than that of CK in all 1/2 nitrogen application + rhizobium treatment. In the seed filling stage, the difference among net photosynthetic rate, transpiration rate, and CK of each treatment were not significant, and the stomatal conductance was higher than CK, which was not treated with nitrogen fertilizer. The chlorophyll content was significantly lower than that of CK, dry weight of leaves was significantly lower than that of CK, but the stem, pod dry weight were not significantly different from CK and seed dry weight had a certain upward trend, seed dry weight of T3 was 24.6% higher than that of CK. At the full maturity, 1/2 nitrogen reduction + inoculation Rhizobium treatment can basically increase the yield of soybean and its constituent factors especially the T3 treatment increased the yield by 9.75% compared with CK.
参考文献/References:
[1]Ainsworth E,YendrekC,Skoneczka J, et al.Acceleratingyield potential in soybean: Potential targets for biotechnological improvement[J].Plant Cell and Environment,2012,35:38-52.
相似文献/References:
[1]刘章雄,李卫东,孙石,等.1983~2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(03):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(03):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(03):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(03):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(03):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(03):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(03):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(03):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(03):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(03):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
备注/Memo
收稿日期:2018-11-23