[1]朱林,左妍妍,曹金山,等.大豆NRT1.2同源基因的生物信息学分析[J].大豆科学,2019,38(03):371-378.[doi:10.11861/j.issn.1000-9841.2019.03.0371]
　ZHU Lin,ZUO Yan-yan,CAO Jin-shan,et al.Bioinformatic Analysis of NRT1.2 Homologous Gene in Soybean[J].Soybean Science,2019,38(03):371-378.[doi:10.11861/j.issn.1000-9841.2019.03.0371]
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大豆NRT1.2同源基因的生物信息学分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第38卷 期数: 2019年03期 页码: 371-378 栏目: 出版日期: 2019-05-20
Title:
Bioinformatic Analysis of NRT1.2 Homologous Gene in Soybean
作者:
朱林; 左妍妍; 曹金山; 王小迪; 杨薇; 王幼宁
（华中农业大学 植物科学技术学院，湖北 武汉 430070）
Author(s):
ZHU Lin;  ZUO Yan-yan;  CAO Jin-shan;  WANG Xiao-di;  YANG Wei;  WANG You-ning
(College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China)
关键词:
大豆; 氮素营养; NRT1.2; 共生固氮; 生物信息学
Keywords:
Soybean;  Nitrogen nutrient;  NRT1.2;  Symbiotic nitrogen fixation;  Bioinformatics
DOI:
10.11861/j.issn.1000-9841.2019.03.0371
文献标志码:
A
摘要:
AtNRT1.2已被证明在模式植物拟南芥中作为低亲和硝酸根转运蛋白参与硝态氮的吸收。为研究其编码基因在大豆中的同源基因，进一步挖掘大豆中参与调控共生固氮的候选基因并为开展其功能研究奠定基础，本研究利用Phytozome、ProtParam、TAIR和SoyBase数据库以及GSDS、TMpred Server、ClustalX 2.1和MEGA 7.0软件，对NRT1.2在大豆中的6个同源蛋白的系统进化关系、基因序列、氨基酸序列、跨膜结构以及表达模式进行生物信息学分析。系统进化分析发现GmNRT1.2a、GmNRT1.2b、GmNRT1.2c和GmNRT1.2d与菜豆和苜蓿等豆科植物的NRT1.2亲缘关系较近。氨基酸序列比对分析发现GmNRT1.2a和GmNRT1.2b相似度较高，且GmNRT1.2s都含有类似的跨膜结构。在表达模式方面，SoyBase的数据显示GmNRT1.2s同源基因的表达存在较明显差别，GmNRT1.2a和GmNRT1.2b在大豆根及根瘤中表达较高。以上生物信息学分析结果暗示GmNRT1.2s可能在参与硝酸盐吸收的同时也介导大豆共生固氮过程。本研究结果为深入探究GmNRT1.2s在氮吸收与共生固氮协同调控大豆氮素供给的分子机制方面提供了一定的数据支持。
Abstract:
AtNRT1.2 functions as a low affinity nitrate transporter involved in nitrae uptake in Arabidopsis. Based on studying the homologous genes of AtNRT1.2 in soybean and identifying putative genes involved in the regulation of symbiotic nitrogen fixation in soybean, it will provide evidence to uncover its’ biological function. In this study, the homologous genes of AtNRT1.2 have been analyzed using different bioinformatic softwares and methods. It was found that GmNRT1.2a, GmNRT1.2b, GmNRT1.2c and GmNRT1.2d showed to have higher similarity with the homologous genes in Phaseolus vulgaris and Medicago truncatula. In addition, the greater the similarity between the amino acid sequences of GmNRT1.2a and GmNRT1.2b were found and showed the closer relationship between them compared with other homologous genes. Similar to AtNRT1.2, all of GmNRT1.2s contained the conserved transmembrane structure. Based on the expression data collected from SoyBase, GmNRT1.2a and GmNRT1.2b appeared to be highly expressed in roots and nodules of soybean. In summary, our results provide clues for the role of GmNRT1.2s in nitrate absorption and symbiotic nitrogen fixation of soybean.

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备注/Memo

收稿日期：2018-12-29

基金项目：国家科技重大专项 （2018ZX08009-19B）；国家自然科学基金（31872873）。
第一作者简介：朱林（1992-），男，硕士，主要从事大豆硝酸盐转运与共生固氮等研究。E-mail：onelin@163.com。
通讯作者：王幼宁（1977-），女，博士，副教授，主要从事大豆共生固氮分子机制研究。E-mail：youningwang@mail.hzau.edu.cn。

更新日期/Last Update: 2019-05-30