[1]简朴,王亚琪,李亚蔚,等.大豆波状卷叶新种质NWL1特性鉴定与基因定位 [J].大豆科学,2018,37(05):690-696.[doi:10.11861/j.issn.1000-9841.2018.05.0690]
　JIAN Pu,WANG Ya-qi,LI Ya-wei,et al.Characterization and Gene Mapping of A Wavy Leaf Line NWL1 in Soybean[J].Soybean Science,2018,37(05):690-696.[doi:10.11861/j.issn.1000-9841.2018.05.0690]
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大豆波状卷叶新种质NWL1特性鉴定与基因定位

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第37卷 期数: 2018年05期 页码: 690-696 栏目: 出版日期: 2018-09-20
Title:
Characterization and Gene Mapping of A Wavy Leaf Line NWL1 in Soybean
作者:
简朴; 王亚琪; 李亚蔚; 孔杰杰; 赵团结
（南京农业大学 大豆研究所/国家大豆改良中心/农业部大豆生物学与遗传育种重点实验室(综合)/作物遗传与种质创新国家重点实验室/江苏省现代作物生产协同创新中心，江苏 南京 210095)
Author(s):
JIAN Pu;  WANG Ya-qi;  LI Ya-wei;  KONG Jie-jie;  ZHAO Tuan-jie
(Soybean Research Institute of Nanjing Agricultural University/ National Center for Soybean Improvement/ Key Laboratory for Biology and Genetic Improvement of Soybean (General), Ministry of Agriculture/ National Key Laboratory for Crop Genetic and Germplasm Enhancement/ Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing 210095, China)
关键词:
大豆; 波状叶; 基因定位; 黄酮醇苷
Keywords:
Soybean;  Wavy leaf;  Gene mapping;  Flavone glycosides
DOI:
10.11861/j.issn.1000-9841.2018.05.0690
文献标志码:
A
摘要:
为给大豆叶发育研究及株型改良提供新的基因资源及相关信息，通过对大豆波状卷叶新材料NWL1的性状评价与基因定位，比较NWL1与野生型之间形态农艺性状、光合生理特性的差异，并对波状卷叶控制基因进行基因定位。结果表明：突变体NWL1植株自第3片复叶起叶缘呈波浪状，切片观测发现其叶脉表皮细胞排列不规则；农艺性状调查发现杂交后代波状卷叶植株的株高及产量构成因子性状值均低于野生型，但也存在单株荚、粒数较多的波状卷叶单株可供选择。2个杂交组合后代的遗传分析表明该波状卷叶性状受两对隐性基因wl1、wl2控制，还发现所有波状卷叶植株均为灰毛，表明茸毛色与波状卷叶基因紧密连锁或共分离。利用W82×NWL1群体F2群体77个隐性单株, 将wl1基因定位在第6号染色体上SSR标记BARCSOYSSR_06_0971和BARCSOYSSR_06_1008之间约1.0 Mb区间。从定位区间的56个候选基因中选出调控黄酮醇苷表达的茸毛色基因Glyma.06G202300，测序发现NWL1等突变材料中该基因第三个外显子中第79个碱基A缺失，致其后氨基酸序列改变。wl2被定位在第7号染色体SSR标记AF186183和BARCSOYSSR_07_1143之间，二者遗传距离为25.1 cM。
Abstract:
In order to evaluate the characteristics and map the target genes of a new wavy leaf mutant, and to provide new genetic resources and useful information for the deep study on leaf development and plant type breeding in soybean. Performance of morphological and photosynthetic characteristics between NWL1 and its wild type line was investigated, and inheritance and gene mapping work was also conducted. It was showed that the third and late growth trifoliate leaves of mutant line NWL1 appeared wavy margin, and the epidermal cells arranged irregularly in comparison with those in wild type line. The values of plant height and yield component trait of F2 mutant plants were lower than those of wild type. However, there were some mutant plants with high pod and seed number for selection. Genetic analysis showed that the mutant phenotype was controlled by two pairs of recessive genes, wl1and wl2, and all mutant plants performed gray pubescence, indicating pleiotropy or tight linkage of these two genes for pubescence color and wavy leaf margin. wl1gene was located on chromosome 06 between SSR markers BALCSOYSSR_06_0971 and BALCSOYSSR_06_1008 with physical distance of about 1.0 Mb using 77 F2 recessive plants from W82×NWL population. There were 56 predicted genes in the wl1mapping region, and the pubescence gene Glyma.06G202300 involved in the expression of flavonol glycosides was selected to sequence. It was found that a base A deletion appeared to change amino acid sequences at the 79th base position in the third exon of Glyma.06G202300. wl2was located on chromosome 07 between the markers AF186183 and BARCSOYSSR_07_1143 with genetic distance of 25.1 cM. No wavy leaf locus was previously reported in the mapping region of wl2.

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备注/Memo

收稿日期：2018-04-27

基金项目：国家重点研发计划（2017YFD0101500）；长江学者和创新团队发展计划（PCSIRT_17R55）；中央高校基本科研业务费专项资金（KYT201801）；江苏省现代作物生产协同创新中心项目（JCIC-MCP）。
第一作者简介：简朴（1991-），女，硕士，主要从事大豆分子育种研究。E-mail: 2015101068@njau.edu.cn。
通信作者：赵团结（1969-），男，博士，教授，主要从事大豆遗传育种研究。E-mail: tjzhao@njau.edu.cn。

更新日期/Last Update: 2018-10-08