[1]邢锦城,孙晨曦,洪立洲,等.大豆转录因子GmbZIP60对非生物胁迫的表达模式分析[J].大豆科学,2018,37(01):45-49.[doi:10.11861/j.issn.1000-9841.2018.01.0045]
　XING Jin-cheng,SUN Chen-xi,HONG Li-zhou,et al.Expression Patterns of Soybean Transcription Factor GmbZIP60 in Response to Abiotic Stresses[J].Soybean Science,2018,37(01):45-49.[doi:10.11861/j.issn.1000-9841.2018.01.0045]
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大豆转录因子GmbZIP60对非生物胁迫的表达模式分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第37卷 期数: 2018年01期 页码: 45-49 栏目: 出版日期: 2018-01-20
Title:
Expression Patterns of Soybean Transcription Factor GmbZIP60 in Response to Abiotic Stresses
作者:
邢锦城 1; 孙晨曦 2; 洪立洲 1; 许 玲 3; 徐照龙 3; 张大勇 3
(1.江苏沿海地区农业科学研究所，江苏 盐城 224002； 2.山东省环境规划研究院，山东 济南 250101； 3.江苏省农业科学院 农业资源与环境研究所，江苏 南京 210094)
Author(s):
XING Jin-cheng 1;  SUN Chen-xi 2;  HONG Li-zhou 1;  XU Ling 3;  XU Zhao-long 3;  ZHANG Da-yong 3
(1.Institute of Agricultural Sciences in Coastal Area of Jiangsu,Yancheng 224002,China; 2.Shandong Academy for Environmental Planning,Jinan 250101,China; 3.Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210094, China)
关键词:
大豆; bZIP转录因子; 非生物胁迫
Keywords:
Soybean;  bZIP transcription factor;  Abiotic stress
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2018.01.0045
文献标志码:
A
摘要:
bZIP基因在植物响应非生物胁迫过程中发挥重要的调控作用。前期试验已经从大豆中克隆到一个bZIP基因GmbZIP60Genebank Accession No:DQ787038)，并成功转化到拟南芥中。本研究在此基础上，利用qRT-PCR和GUS组织化学染色法研究了高温(37℃)、低温(4℃)、干旱(PEG6000)、NaCl（200 mmol·L -1）和ABA（100 mg·L -1）处理对GmbZIP60表达的影响。结果表明：高温、低温、干旱和NaCl胁迫处理下，GmbZIP60表达量显著降低，分别变为对照组的0.07，0.25，0.36和0.13倍，而ABA处理对GmbZIP60表达量影响不显著，GUS染色结果与荧光定量PCR结果一致。研究认为，GmbZIP60可能以负调控的方式参与大豆抗高盐、干旱、高温、低温等非生物胁迫的应答反应。本研究为进一步鉴定和克隆大豆逆境应答关键基因、揭示大豆抗逆分子机制和大豆抗逆性基因工程改良提供了有益借鉴。
Abstract:
bZIP genes play significant roles in the response to abiotic stresses. We have cloned a bZIP gene named as GmbZIP60 (Genebank,Accession, No:DQ787038) from soybean, and transformed it into Arabidopsis thaliana. Then on the base of these, this paper studied the GmbZIP60 gene expression profiling in Arabidopsis seedlings under the treatments of PEG, high-temperature（37℃）, low-temperature（4℃）, high-salt(PEG6000) and ABA(100 mg·L -1) by qRT-PCR and GUS histochemical staining. The fluorescence quantitative RT-PCR results showed that the expression of GmbZIP60 genes were down-regulated by PEG, high-temperature, low-temperature and salt treatments respectively. The results of GUS staining were agree with fluorescence quantitative RT-PCR. This indicated that GmbZIP60 might be involved in the responsing to abiotic stresses such as high salinity, drought, high temperature and low temperature. This study laid the foundation for revealing the molecular mechanism of soybean resistance and genetic engineering improvement of soybean resistance.

参考文献/References:

［1］ 盖钧镒.发展我国大豆遗传改良事业解决国内大豆供给问题［J］.中国工程科学,2003,5(5):1-5.(Gai J Y.Expanding and enhancing the research allocation on soybean breeding and genetics for the establishment of market supply based on domestic production［J］.Engineering Science,2003,5(5):1-5.)

［2］ Perez-Rodriguez P, Riano-Pachon D M, Correa L G, et al. PlnT-FDB: Updated content and new features of the plant transcription factor database［J］.Nucleic Acids Research,2010,38:822-827.
［3］ Xu Z S, Chen M, Li L C,et al. Functions and application of the AP2/ERF transcription factor family in crop improvement［J］.Journal of Integrative Plant Biology, 2011, 53: 570-575.
［4］ Kim S, Kang J Y, Cho D I,et al.ABF2, an ABRE-binding bZIP factor, is an essential component of glucose signaling and its overexpression affects multiple stress tolerance［J］.The Plant Journal, 2004, 40(1): 75-87.?
［5］ Jakoby M,Weisshaar B,Droge-Laser W, et al. bZIP transcription factors in Arabidopsis［J］. Trends in Plant Science,2002,7(3):106-111.
［6］ Baloglu M C, Eldem V, Hajyzadeh M, et al. Genome wide analysis of the bZIP transcription factors in cucumber［J］.PLoS One,2014,9(4):e96014.?
［7］ Nijhawan A, Jain M, Tyagi A K, et al. Genomic survey and gene expression analysis of the basic leucine zipper transcription factor family in rice［J］.Plant Physiology, 2008,146(2):330-350.
［8］ Wang J Z，Ghou J X，Zhang B L, et al. Genome-wide expansion and expression divergent of the basic: Leuoine zipper transition factors in higher plants with an emphasis on sorghum［J］.Journal of Integrative Plant Biology,2011,53(3):212-231.
［9］ Wei K, Chen J, Wang Y,et al. Genome-wide analysis of bZIP-encoding genes in maize［J］. DNA Research,2012,19(6):463-476.
［10］Liao Y, Zou H F, Wei W, et al. Soybean GmbZIP44, GmbZIP62 and GmbZIP78 genes function as negative regulator of ABA signaling and confer salt and freezing tolerance in transgenic Arabidopsis［J］.Planta, 2008,228(2):225-240.
［11］Zhang L, Zhang L, Xia C, et al. A novel wheat bZIP transcription factor TabZIP60 confers multiple abiotic stress tolerances in transgenic Arabidopsis［J］.Plant Physiology,2015,153(4):538-554.
［13］Chen H, Chen W, Zhou J, et al. Basic leucine zipper transcription factor OsbZIP16 positively regulates drought resistance in rice［J］. Plant Science,2012,193-194:8-17.
［14］Liu C, Mao B, Ou S, et al. OsbZIP71 a bZIP transcription factor confers salinity and drought stress response in rice［J］.Plant Molecular Biology, 2014,84(12):19-36.
［15］Wang Y, Gao C, Liang Y, et al. A novel bZIP gene from Tamarix hispida mediates physiological responses to salt stress in tobacco plants［J］.Journal of Plant Physiology,2010,167: 222-230.
［16］Xu L, Xu Z L, Liu X Q, et al. The subcellular localization and ectopic expression analysis in Arabidopsis of soybean GmbZIP60 gene［J］.Journal of Plant Biochemistry and Biotechnology, 2015, 24 (1):9-17.
［17］Zhang X, Wang L, Meng H, et al. Maize ABP9 enhances tolerance to multiple stresses in transgenic Arabidopsis by modulating ABA signaling and cellular levels of reactive oxygen species［J］.Plant Molecular Biology,2011,75(4-5):365-378.
［18］Zou J, Liu C, Liu A, et al. Overexpression of OsHsp17.0 and OsHsp 23.7 enhances drought and salt tolerance in rice ［J］.Journal of Plant Physiology, 2012,169:628-635.
［19］Zhou T, Yang X, Wang L, et al. GhTZF1 regulates drought stress responses and delays leaf senescence by inhibiting reactive oxygen species accumulation in transgenic Arabidopsis［J］.Plant Molecular Biology, 2014, 85 (1-2):163-177.
［20］Liao Y, Zou H F, Wei W, et al. Soybean GmbZIP44〖STBZ〗,GmbZIP62 and GmbZIP78 genes function as negative regulator of ABA signaling and confer salt and freezing tolerance in transgenic Arabidopsis［J］.Planta,2008, 228:225-240.
［21］Liu C, Wu Y, Wang X, et al. A bZIP transcription factor OsbZIP52/RISBZ5: A potential negative regulator of cold and drought stress response in rice［J］.Planta,2012,235 (6): 1157-1169.?
［22］Zou M, Guan Y, Ren H, et al. A bZIP transcription factor, OsABI5, is involved in rice fertility and stress tolerance［J］.Plant Molecular Biology, 2008, 66 (6):675-683.

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备注/Memo

收稿日期：2017-08-09

基金项目：江苏省农业科技自主创新项目［CX(15)1005］；江苏省自然科学基金面上项目（BK20151301）。
第一作者简介：邢锦城（1983-），男，硕士，助理研究员，主要从事植物逆境生理方面研究。E-mail：sdauxxx@163.com。
通讯作者：张大勇（1979-），男，博士，副研究员，主要从事植物抗逆功能基因组学研究。E-mail：cotton.z@126.com。

更新日期/Last Update: 2018-03-13