[1]刘丹丹,刘畅,王琳,等.降解菌对大豆生长前期莠去津毒害作用的缓解[J].大豆科学,2017,36(06):938-942.[doi:10.11861/j.issn.1000-9841.2017.06.0938]
　LIU Dan-dan,LIU Chang,WANG Lin,et al.Alleviation Effects of Degrading Bacteria on Atrazine Toxicity in Early Stage of Soybean Growth[J].Soybean Science,2017,36(06):938-942.[doi:10.11861/j.issn.1000-9841.2017.06.0938]
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降解菌对大豆生长前期莠去津毒害作用的缓解

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第36卷 期数: 2017年06期 页码: 938-942 栏目: 出版日期: 2017-11-20
Title:
Alleviation Effects of Degrading Bacteria on Atrazine Toxicity in Early Stage of Soybean Growth
作者:
刘丹丹; 刘畅; 王琳; 赵晋; 丁永昌
(沈阳化工大学 环境与安全工程学院，辽宁 沈阳 110142)
Author(s):
LIU Dan-dan;  LIU Chang;  WANG Lin;  ZHAO Jin;  DING Yong-chang
(Department of Environmental and Safety Engineering, Shenyang University of Chemical Technology, Shenyang 110142, China)
关键词:
莠去津; 大豆; 生物修复
Keywords:
Atrazine;  Soybean;  Bioremediation
DOI:
10.11861/j.issn.1000-9841.2017.06.0938
文献标志码:
A
摘要:
为明确降解菌(Enterobacter sp)对大豆莠去津毒害作用的缓解能力，以辽豆15为试验材料，通过室内种子萌发和幼苗盆栽试验方法，对大豆生长前期的生长和生理指标进行测定。结果表明：与莠去津处理比较，降解菌能显著提高大豆种子的发芽率、发芽指数、种子活力指数和α-淀粉酶活性，减少电解质外渗和脯氨酸积累。同时，降解菌对大豆幼苗生长有一定的促进作用，大豆经菌液处理后，其根系相关发育指标、胚芽和子叶重量均显著高于莠去津处理。可见，试验菌株能有效缓解生长前期莠去津对大豆的毒害作用。
Abstract:
Atrazine toxicity alleviation of degrading bacteria (Enterobacter sp) was investigated by determination of growth and physiological indexes of soybean, utilizing seed germination and pot experiments in the early stage of soybean growth. The results showed that the degrading bacteria could significantly increase the germination rate, germination index, seed vigor index and α-amylase activity of soybean seeds, reduce the electrolyte extravasation and the accumulation of proline.Degradation bacteria could promote the growth of soybean seeding- After treated with bacterial solution, root development index, embryo and cotyledon weight were significantly increased compared with atrazine treatment. Test strains could effectively relieve the toxicity of atrazine.

参考文献/References:

［1］Johnson T A, Ellsworth T R, Hudson R J M, et al. Diffusion limitation for atrazine biodegradation in soil ［J］. Advances in Microbiology, 2013, 5: 412-420.

［2］Solomon R D J, Kumar A, Satheeja S V. Atrazine biodegradation efficiency, metabolite detection, and trzD gene expression by enrichment bacterialcultures from agricultural soil ［J］. Journal of Zhejiang Universit-Science B (Biomedicine & Biotechnology), 2013, 14(12):1162-1172.
［3］Udikovic＇-Kolic N, Hrak D, Devers M, et al. Taxonomic and functional diversity of atrazine-degrading bacterial communities enriched from agrochemical factory soil ［J］. Journal of Applied Microbiology, 2010, 109:355-367.
［4］周游. 阿特拉津对小麦幼苗的生物毒性［D］. 南京:南京农业大学, 2012:6-11.(Zhou Y. The bio-toxcity of atrazine on the stand wheat seedlings ［D］. Nanjing: Nanjing Agricultural University, 2012:6-11.)
［5］王锦, 陈雅琳, 刘丹竹, 等. 玉米田残留除草剂对后茬大豆苗期药害的研究［J］. 大豆科技, 2016(5):23-26. (Wang J, Chen Y L, Liu D Z, et al. Phytotoxicity of herbicide residued in corn field on succeeding soybean seedlings ［J］. Soybean Science & Technology, 2016(5):23-26. )
［6］宋日, 刘利, 马丽艳, 等. 阿特拉津对不同种子大小品种大豆的危害［J］. 中国油料作物学报, 2013, 35(2):207-210. (Song R, Liu L, Ma L Y, et al. Effect of atrazine on soybeans with various seed size varieties ［J］. Chinese Journal of Oil Crop Sciences, 2013, 35(2):207-210.)
［7］Fan X, Song F.Bioremediation of atrazine: Recent advances and promises ［J］. Journal of Soils Sediments, 2014, 14:1727-1737.
［8］刘丹丹, 梁彦秋, 刘长风, 等. 莠去津高效降解菌的鉴定和降解特性分析［J］. 农药, 2016(5):340-342,355. (Liu D D, Liang Y Q, Liu C F, et al. Identification and characterization of an antrazine degrading bacterium ［J］. Agrochemicals, 2016, 5:340-342,355.)
［9］周岚, 杨永, 王占海, 等. 玉米-大豆轮作及氮肥施用对土壤细菌群落结构的影响［J］. 作物学报, 2013, 39(11):2016-2022. (Zhou L, Yang Y, Wang Z H, et al. Influence of maize-soybean rotation and N fertilizer on bacterial community composition ［J］. Acta Agronomica Sinica, 2013,39(11):2016-2022.)
［10］陈乐, 赵莹莹, 王媛媛, 等. 细菌SnebYK诱导大豆抗阿特拉津的效果评价［J］. 中国油料作物学报, 2014, 34(5):641-647. (Chen L, Zhao Y Y, Wang Y Y, et al. Evaluation of bacterial strain SnebYK induced soybean against atrazine ［J］. Chinese Journal of Oil Crop Sciences, 2014, 34(5):641-647.)
［11］Wang H, Liu Y, Li J, et al. Biodegradation of atrazine by Arthrobacter sp. C3, isolated from the herbicide-contaminated corn field ［J］. International Journal Environmental Science and Technology, 2016, 13:257-262.?
［12］罗在全, 段玉玺, 王媛媛, 等. 细菌SnebYK发酵液诱导大豆抗阿特拉津的研究［J］. 大豆科学, 2010, 29(2):284-287. (Luo Z Q, Duan Y X, Wang Y Y, et al. Fermentation broth of bacteria SnebYK induced anti-atrazine effect in soybean［J］. Soybean Science, 2010, 29(2):284-287.)
［13］莫金钢, 马建, 张丽辉, 等. 干旱胁迫对大豆种子萌发的影响［J］. 大豆科学, 2014, 33(5):701-704. (Mo J G, Ma J, Zhang L H, et al. Effent of drought stress on germination of soybean ［J］. Soybean Science, 33(5):701-704.)
［14］陈玉生. 外源谷胱甘肽对大豆种子萌发过程中铜毒害的缓解效应［J］. 大豆科学, 2012, 30(2):245-251. (Chen Y S. Alleviation effects of exogenous glutathione on copper toxicity during soybean seeds germination ［J］. Soybean Science, 2012, 30(2):245-251.)
［15］张志良. 植物生理学实验指导(第二版) ［M］. 北京:高等教育出版社, 1993. ( Zhang Z L. Plant physiology experiments guidance (second edition) ［M］. Beijing: Higer Education Press, 1993.)
［16］薛应龙. 植物生理学实验［M］. 北京：高等教育出版社, 1990. (Xue Y L. Plant physiology experiments ［M］. Beijing: Higer Education Press, 1990.)
［17］Giannopo L C N, Ries S K. Superoxide dismutases: I. occurrence in higer plants ［J］. Plant Physiology, 1977, 59(2):309-314.
［18］马兵兵, 姜昭, Kehinde O E, 等. 狼尾草根系对阿特拉津长期胁迫的氧化应激响应［J］. 生态毒理学报, 2016, 11(6):214-222. (Ma B B, Jiang Z, Kehinde O E, et al. Oxidative stress response in root of Pennisetum americanum L., (cv. K. Schum) to long term atrazine exposure［J］. Asian Journal of Ecotoxicology, 2016, 11(6):214-222.)

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备注/Memo

基金项目：国家自然科学基金(31400481)；辽宁省博士科研启动项目(20141086)。

第一作者简介：刘丹丹(1981-)，女，博士，讲师，现主要从事环境污染治理与修复研究。E-mail:liudandan.553@163.com。

更新日期/Last Update: 2018-01-28