PDF下载 +分享

微信公众号：大豆科学

[1]王铎,张力,范素杰,等.大豆主要脂肪酸含量近红外模型的建立[J].大豆科学,2017,36(02):295-300.[doi:10.11861/j.issn.1000-9841.2017.02.0295]
　WANG Duo,ZHANG Li,FAN Sujie,et al.Establishment of NIRS Model for the Main Fatty Acids in Soybean[J].Soybean Science,2017,36(02):295-300.[doi:10.11861/j.issn.1000-9841.2017.02.0295]

点击复制

大豆主要脂肪酸含量近红外模型的建立

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第36卷期数: 2017年02期页码: 295-300 栏目: 出版日期: 2017-03-20

Title:: Establishment of NIRS Model for the Main Fatty Acids in Soybean

作者:: 王铎¹; 张力²; 范素杰¹; 韩笑¹; 吴晗¹; 王丕武¹; 姚丹¹; 张君¹; 1吉林农业大学农学院，吉林长春130118；
2双辽市农业局，吉林双辽 136400

Author(s):: WANG Duo¹; ZHANG Li²; FAN Sujie¹; HAN Xiao¹; WU Han¹; WANG Piwu¹; YAO Dan¹; ZHANG Jun¹; 1Agriculture College of Jilin Agricultural University, Changchun 130118, China;
2Agricultural Bureau of Shuangliao City, Shuangliao 136400, China

关键词:: 大豆; 脂肪酸含量; 近红外模型

Keywords:: Soybean; Fatty acid content; Near infrared model

DOI:: 10.11861/j.issn.1000-9841.2017.02.0295

文献标志码:: A

摘要:: 为提高大豆脂肪酸品种的育种进程，使用气相色谱法测量289份大豆籽粒样品的脂肪酸含量，并用近红外谷物品质分析仪NIRFIEX N500采集大豆样品近红外光谱值，采用主成分回归法将化学试验测定的数值与采集的光谱曲线对应拟合整理分析建立定标模型。每种脂肪酸建立11个定标标准，选取决定系数定标Q值中最高的数值为定标模型。结果显示5种脂肪酸定标模型的适宜Q值分别为：棕榈酸08235、硬脂酸08541、油酸08196、亚油酸08291、亚麻酸08363。最后验证结果表明，近红外法测量值与化学试验测定值误差均在1.5%误差范围之内，证明所建立模型质量较好，具有使用价值，可用于大豆育种材料脂肪酸含量的快速测量。

Abstract:: In order to improve the efficiency of soybean fatty acid breeding and to speed up the breeding process, the fatty acid content of 289 soybean grain samples measured by GC and NIRFIEX N500 was used to collect the NIR spectra of soybean samples Using the principal component regression (prineipal component regession, referred to as PCR)， the calibration model was established by fitting and analyzing the value of the chemical experiment and the corresponding spectral curve Eleven calibration criteria were established for each fatty acid, and the highest value of calibration coefficient Q was selected as the calibration model The results showed the fit calibration Q value for the calibration model The fit Q value of palmitic acid was 08235, stearic acid was 08541, oleic acid was 08196, linoleic acid was 08291, and linolenic acid was 08363, respectively Validation results demonstrated that the errors between the near infrared detection and chemical test results were within 1.5%, which well explained the model and could be used to measure the fatty acid contents of soybean in breeding

参考文献/References:

［1］宋晓昆，张颖君，闫龙，等大豆脂肪酸组份相关、变异特点分析［J］. 华北农学报,2010(S2):68-73(Song X K,Zhang Y J,Yan L,et al A study on correlation and variability of fatty acid composition contents of soybean cultivars［J］.Acta Agriculturae BorealiSinica,2010(S2):68-73)

［2］任波,李毅大豆种子脂肪酸合成代谢的研究进展［J］. 分子植物育种,2005(3):301-306(Ren B, Li Y Research advances on fatty acid biogynthesis metabolism in soybean seed［J］.Molecular Plant Breeding,2005,3(3):301-306)

［3］邹筱,韩粉霞,陈明阳,等大豆脂肪酸主要组分含量QTL定位［J］. 作物学报,2014,40(9):1595-1603(Zou X,Han F X,Chen M Y,et al Quantitative trait loci associated with major fatty acid components in soybean［J］.Acta Agronomica Sinica,2014,40(9):1595-1603)

［4］王晓燕,张彩英,贾晓艳河北省大豆品种脂肪酸组成与含量分析［J］. 河北农业大学学报,2007(2):15-18(Wang X Y, Zhang C Y, Jia X YAnalysis of fatty acids composition and content in soybean varieties in Hebei province［J］. Journal of Agricultural University of Hebei,2007,30(2):15-18)

［5］尹田夫大豆油脂脂肪酸改良与生化育种策略［J］. 大豆科学,1988,7(1):75-79 (Yin T FImprovement on fatty acid of ail lipid and strategy of biochemistry breeding in soybean［J］. Soybean Science,1988,7(1):75-79)

［6］李志香,沈翠平多不饱和脂肪酸对人体的作用［J］. 生物学通报,1998,33(1):11-12(Li Z X,Shen C P Role of polyunsaturated fatty acids in human body［J］. Bulletin of Biology,1998,33(1):9-11)

［7］徐杰,胡国华,张大勇大豆种子脂肪酸组分的研究进展［J］. 大豆科学,2005,24(1):61-66 (Xu J, Hu G H, Zhang D Y Study advance on fatty acid composition of soybean［J］.Soybean Science,2005,24(1):61-66)

［8］张颖君,高慧敏,蒋春志,等大豆种子脂肪酸含量的快速测定［J］. 大豆科学,2008,27(5):859-862（Zhang Y J, Gao H M, Jiang C Z, et al Fast analysis on fatty acids of soybean seed by gas chromatography［J］. Soybean Science, 2008,27(5):859-862）

［9］McClure W F Near infrared spectroscopy: The giant is running strong［J］. Analytical Chemistry,1994,66(1):43A-53A

［11］柴玉华,谭克竹基于近红外分析技术检测大豆脂肪酸含量的研究［J］. 农业工程学报,2007,23(1):238-241(Chai Y H,Tan K Z Measurement of soybean fatty acid content by near infrared spectroscopy［J］. Transactions of the CSAE, 2007, 23(1): 238-241)

［12］梁丹应用近红外光谱分析技术定量检测植物油脂肪酸含量的研究［J］. 安徽农业科学,2012,40(30):14933-14936(Liang D Quantitative detection research for the fatty acid of vegetable oil using near infrared spectroscopy［J］.Journal of Anhui Agricultural Sciences,2012,40(30):14933-14936)

［13］闫龙,蒋春志,于向鸿,等大豆粗蛋白、粗脂肪含量近红外检测模型建立及可靠性分析［J］. 大豆科学,2008,27(5):833-837（Yan L,Jiang C Z,Yu X H,et al Development and reliability of near in frared spectroscopy (NIS) models of protein and oil content in soybean［J］.Soybean Science,2008,27（5）:833-837）

5］尹田夫大豆油脂脂肪酸改良与生化育种策略［J］. 大豆科学,1988,7(1):75-79 (Yin T FImprovement on fatty acid of ail lipid and strategy of biochemistry breeding in soybean［J］. Soybean Science,1988,7(1):75-79)

［9］McClure W F Near infrared spectroscopy: The giant is running strong［J］. Analytical Chemistry,1994,66(1):43A-53A

［10］徐广通,袁洪福,陆婉珍 现代近红外光谱技术及应用进展［J］. 光谱学与光谱分析,2000,20(2):134-142 (Xu G T, Yuan H F, Lu W Z Development of modern near infrared spectroscopic techniques and its applications［J］.Spectroscopy and Spectral Analysis,2000,20(2):134-142)［11］柴玉华,谭克竹基于近红外分析技术检测大豆脂肪酸含量的研究［J］. 农业工程学报,2007,23(1):238-241(Chai Y H,Tan K Z Measurement of soybean fatty acid content by near infrared spectroscopy［J］. Transactions of the CSAE, 2007, 23(1): 238-241)［12］梁丹应用近红外光谱分析技术定量检测植物油脂肪酸含量的研究［J］. 安徽农业科学,2012,40(30):14933-14936(Liang D Quantitative detection research for the fatty acid of vegetable oil using near infrared spectroscopy［J］.Journal of Anhui Agricultural Sciences,2012,40(30):14933-14936)［13］闫龙,蒋春志,于向鸿,等大豆粗蛋白、粗脂肪含量近红外检测模型建立及可靠性分析［J］. 大豆科学,2008,27(5):833-837（Yan L,Jiang C Z,Yu X H,et al Development and reliability of near in frared spectroscopy (NIS) models of protein and oil content in soybean［J］.Soybean Science,2008,27（5）:833-837）

［14］范胜栩,李斌,孙君明,等气相色谱方法定量检测大豆5种脂肪酸［J］. 中国油料作物学报,2015（4）:548-553（Fan S Y,Li B, Sun J M,et al A quantitative gas chromatographic method for determination of soybean seed fatty acid components［J］. Chinese Journal of Oil Crop Sciences,2015(4):548-553)

［15］李勇,魏益民,王锋影响近红外光谱分析结果准确性的因素［J］. 核农学报,2005(3):236-240 (Li Y,Wei Y M,Wang F Affecting factors on the accuracy of near infrared spectroscopy analysis［J］.Acta Agriculturae Nucleatae Sinica,2005(3):236-240)

［16］刘青格近红外光谱的信息特征提取［D］.镇江:江苏大学,2003(Liu Q G The signal features extraction of Near infrared Spectroscopy［D］.Zhenjiang:Jiangsu University,2003)

［17］褚小立,袁洪福,陆婉珍基础数据准确性对近红外光谱分析结果的影响［J］. 光谱学与光谱分析,2005,25(6):886-889(Chu X L, Yuan H F,Lu W Z Progress and application of spectral data pretreatment and wavelength selection methods in NIR analytical technique［J］. Progress in Chemistry,2005,25(6):886-889)

［18］熊英近红外光谱的原理及应用［J］. 中山大学研究生学刊(自然科学医学版),2013(2):16-30(Xiong Y The principle and application of near infrared spectroscopy［J］.Journal of the Graduates Sun Yat Sen University(Natural Sciences & Medicine edition),2013(2):16-30)

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(02):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(02):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(02):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(02):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(02):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(02):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(02):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(02):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(02):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(02):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]赵雪,杜雪,孙晶,等.多环境大豆种质资源脂肪酸组分评价及其与农艺性状的相关分析[J].大豆科学,2014,33(03):353.[doi:10.11861/j.issn.1000-9841.2014.03.0353]
　ZHAO Xue,Du Xue,SUN Jing,et al.Relation Analysis of the Fatty Acidcomponent Content of Soybean Germplasm and Agronomic Trait[J].Soybean Science,2014,33(02):353.[doi:10.11861/j.issn.1000-9841.2014.03.0353]
[12]李侠,常玮,韩英鹏,等.大豆种子脂肪酸含量的遗传分析[J].大豆科学,2009,28(03):403.[doi:10.11861/j.issn.1000-9841.2009.03.0403]
　LI Xia,CHANG Wei,HAN Ying-peng,et al.Genetic Analysis on Fatty Acid Composition Contents in Soybean Seed[J].Soybean Science,2009,28(02):403.[doi:10.11861/j.issn.1000-9841.2009.03.0403]
[13]王茜,刘兵强,贾馨元,等.RIL群体中大豆籽粒脂肪酸组分QTL分析[J].大豆科学,2023,42(04):416.[doi:10.11861/j.issn.1000-9841.2023.04.0416]

备注/Memo

基金项目：吉林省科技发展计划项目(20150204004NY)；吉林省省级粮食生产发展专项(2015001)。

第一作者简介：王铎(1991-)，男，硕士，主要从事大豆分子育种研究。E-mail：499077344@qq.com。

更新日期/Last Update: 2017-05-14