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[1]张国正,邢兴华,王幸,等.不同时期喷施APK9对大豆受酸雨胁迫的缓解效应[J].大豆科学,2017,36(02):280-287.[doi:10.11861/j.issn.1000-9841.2017.02.0280]
　ZHANG Guozheng,XING Xinghua,WANG Xing,et al.Alleviative Effects of Spraying Plant Growth Regulator APK9 on Acid Rain Stressed Soybean Plants at Different Growth Stages[J].Soybean Science,2017,36(02):280-287.[doi:10.11861/j.issn.1000-9841.2017.02.0280]

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不同时期喷施APK9对大豆受酸雨胁迫的缓解效应

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第36卷期数: 2017年02期页码: 280-287 栏目: 出版日期: 2017-03-20

Title:: Alleviative Effects of Spraying Plant Growth Regulator APK9 on Acid Rain Stressed Soybean Plants at Different Growth Stages

作者:: 张国正¹; 邢兴华²; 王幸²; 何小红¹; 江海东¹; 喻德跃¹; 1南京农业大学大豆研究所/国家大豆改良中心/作物遗传与种质创新国家重点实验室，江苏南京 210095；?
2江苏徐淮地区徐州农业科学研究所，江苏徐州 221131

Author(s):: ZHANG Guozheng¹; XING Xinghua²; WANG Xing²; HE Xiaohong¹; JIANG Haidong¹; YU Deyue¹; 1 Soybean Research Institute/National Center for Soybean Improvement/State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China;
2 Xuzhou Institute of Agricultural Sciences of the Xuhuai District of Jiangsu Province, Xuzhou 221121, China

关键词:: 酸雨缓解剂APK9; 大豆; 缓解; 胁迫

Keywords:: Simulated acid rain; Soybean; APK 9; Alleviate; Stress

DOI:: 10.11861/j.issn.1000-9841.2017.02.0280

文献标志码:: A

摘要:: 为探讨叶面喷施新型酸雨缓解剂APK9对酸雨胁迫大豆毒害效应的缓解效果，以徐豆18为供试材料，研究了不同生育时期喷施APK9处理对pH35酸雨胁迫下大豆生长的影响。结果表明：pH35酸雨自开花期开始显著抑制了大豆植株的生长，进而降低了产量。开花期和结荚期喷施APK9可明显缓解大豆的酸雨胁迫，氮积累量、磷积累量、叶片色素含量、抗氧化酶活性等显著高于酸雨胁迫的大豆，丙二醛（MDA）含量显著低于酸雨处理大豆。然而，苗期和鼓粒期喷施APK9，缓解效应下降，叶绿素含量、生物量、产量等下降，而MDA含量上升。可见在适宜时期喷施APK9对酸雨胁迫下的大豆植株具有一定的缓解效应，而过早或过晚喷施效果较小，在试验中以开花期喷施的效果最佳。

Abstract:: Acid rain is considered to be the top ten environment concerns in the world In a field experiment, soybean ( Glycine max L ) cultivar Xuzhou 18 was subjected to simulated acid rain (AR, pH3-5), followed by foliar application of APK 9 consisting of ascorbic acid (AsA) and polyaspartic acid The alleviative effect of APK 9 on AR stress in soybean plants was then investigated The results showed that AR obviously depressed growth after flowering stage and finally reduced soybean yield Chlorophyll content, nitrogen accumulation, phosphorus accumulation, AsA content, and antioxidative enzyme activities in AR stressed seedlings were enhanced, while malonyldialdehyde (MDA) content was repressed by application of APK 9 in flowering stage and podding stage However, chlorophyll content in leaf, biomass and yield reduced while MDA content increased when APK 9 was sprayed in seedlings stage and seed filling stage Thus, AR stress on soybean plants was effectively alleviated by foliar application of APK 9 in flowering stage and podding stage and the alleviation effect could be depressed by earlier or later application of APK 9 Here, spraying APK 9 in flowering stage had the greatest effect on AR stressed plants

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备注/Memo

基金项目：国家自然科学基金(31271749)；江苏省科技支撑计划(BE2012747)。

第一作者简介：张国正(1963-)，男，博士，主要从事大豆耐酸雨抗性机理及优异基因挖掘研究。E-mail：zgz@njau.edu.cn。

更新日期/Last Update: 2017-05-14