[1]朱治佳,赵雪,罗健,等.基因拷贝数变异分析法研究大豆胞囊线虫病抗性位点qSCN33抗性相关基因[J].大豆科学,2017,36(02):199-208.[doi:10.11861/j.issn.1000-9841.2017.02.0199]
　ZHU Zhijia,ZHAO Xue,LUO Jian,et al.Research on the Resistance Genes Related to Soybean Cyst Nematode Resistance Loci qSCN33 with the Copy Number Variation Analysis[J].Soybean Science,2017,36(02):199-208.[doi:10.11861/j.issn.1000-9841.2017.02.0199]
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基因拷贝数变异分析法研究大豆胞囊线虫病抗性位点qSCN33抗性相关基因

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第36卷 期数: 2017年02期 页码: 199-208 栏目: 出版日期: 2017-03-20
Title:
Research on the Resistance Genes Related to Soybean Cyst Nematode Resistance Loci qSCN33 with the Copy Number Variation Analysis
作者:
朱治佳; 赵雪; 罗健; 臧紫薇; 韩英鹏; 李文滨
（东北农业大学 大豆生物学教育部重点实验室，农业部北方大豆生物学与遗传育种区域重点实验室， 黑龙江 哈尔滨 150030）
Author(s):
ZHU Zhijia;  ZHAO Xue;  LUO Jian;  ZANG Ziwei;  HAN Yingpeng;  LI Wenbin
（Chinese Education Ministry′s Key Laboratory of Soybean Biology, Key Laboratory of Northeastern Soybean Biology and Breeding/Genetics of Chinese Agriculture Ministry, Northeast Agricultural University, Harbin 150030, China）
关键词:
大豆; 胞囊线虫抗性; 荧光定量PCR; 拷贝数变异
Keywords:
Soybean;  Cyst nematode resistance;  Copy number variation;  Real time quantitative PCR
DOI:
10.11861/j.issn.1000-9841.2017.02.0199
文献标志码:
A
摘要:
基因拷贝数变异（CNVs）是引起大豆品种对胞囊线虫抗性差异的因素之一，以抗大豆胞囊线虫病种质东农L10以及感病种质黑农37、绥农10和绥农14为试验材料，利用实时荧光定量PCR法对大豆胞囊线虫抗性QTL位点qscn33内27个编码基因进行拷贝数变异分析，F测验表明15个目的基因在不同品种间存在拷贝数变异；抗感病品种PCR产物丰度相对值分析结果表明9个目的基因拷贝数在抗感病种质间存在显著的差异，推测其功能是通过编码抗病蛋白和富亮氨酸蛋白来实现大豆对大豆胞囊线虫相关抗性。
Abstract:
Gene copy number variants (CNVs) can cause the difference in soybean resistance to soybean cyst nematode (SCN) In this study, four soybean germplasms including Dongnong L10(resistant to SCN), Heinong 37, Suinong 10 and Suinong 14 (susceptible to SCN) were selected to analyze the CNVs of 27 genes in the genomic region of SCN resistance related quantitative trait loci(QTL) ‘qscn33’ using the method of real time quantitative PCR The result of Ftest showed that the copy number of 15 genes were different among four soybean germplasms A total of 9 genes showed significant difference in copy number between Dongnong L10 and the other three susceptible germplasms It was speculated that its function was accomplished by coding disease resistant protein and leucine rich protein for SCN resistance

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备注/Memo

基金项目： 省自然科学基金面上项目（C2015011）；国家自然科学基金（31301339,31671717）；黑龙江东北农业大学学术骨干计划项目（15XG04）；东北农业大学青年才俊计划项目（14QC27）。

第一作者简介：朱治佳（1990-），男，硕士，主要从事分子辅助育种研究。E-mail：m15546077313@163.com。

更新日期/Last Update: 2017-05-14