[1]周淑莉,李冬梅,杨玉莹,等.大豆GmAYI-基因的克隆及初步功能预测[J].大豆科学,2016,35(06):1037-1042.[doi:10.11861/j.issn.1000-9841.2016.06.1037]
　ZHOU Shu-li,LI Dong-mei,YANG Yu-ying,et al.Molecular Cloning and Preliminary Functional Prediction of GmAYI in Soybean[J].Soybean Science,2016,35(06):1037-1042.[doi:10.11861/j.issn.1000-9841.2016.06.1037]
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大豆GmAYI-基因的克隆及初步功能预测

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年06期 页码: 1037-1042 栏目: 出版日期: 2016-11-20
Title:
Molecular Cloning and Preliminary Functional Prediction of GmAYI in Soybean
作者:
周淑莉; 李冬梅; 杨玉莹; 张伟娜; 李文滨; 张彬彬
（东北农业大学 大豆生物学教育部重点实验室/农业部东北大豆生物学与遗传育种重点实验室， 黑龙江 哈尔滨 150030）
Author(s):
ZHOU Shu-li ;  LI Dong-mei ; YANG Yu-ying;  ZHANG Wei-na;  LI Wen-bin;  ZHANG Bin-bin
（Key Laboratory of Soybean Biology in Chinese Ministry of Education/Key Laboratory of Soybean Biology and Breeding/Genetics of Chinese Agriculture Ministry，Northeast Agricultural University, Harbin 150030, China）
关键词:
大豆; GmAYI-基因; 相关性; 荧光定量PCR
Keywords:
Soybean;  GmAYI gene;  Correlation;  Quantitative PCR
DOI:
10.11861/j.issn.1000-9841.2016.06.1037
文献标志码:
A
摘要:
根据拟南芥At1G74730基因的保守序列设计引物，利用RT-PCR技术在大豆中克隆得到GmAYI基因。对GmAYI基因的结构域和启动子元件进行分析，结果表明：大豆GmAYI基因位于4号染色体上，含跨膜结构域，属于膜蛋白。该基因启动子区域含有激素相关应答元件和胚乳表达所必须的顺式作用元件。推测该基因可能参与调控大豆生长发育及外界环境胁迫应答等过程。进而对14个大豆品种中GmAYI基因的转录水平进行了定量分析，对GmAYI基因在14个大豆品种中的转录水平与单株粒数、单株粒重、百粒重、株高等产量相关性状进行相关性分析，结果表明：GmAYI基因的表达量与百粒重呈显著负相关，推测GmAYI可能是参与大豆产量相关性状调控的基因。
Abstract:
The primers were designed according to the conserved sequence of Arabidopsis At1G74730 gene.The GmAYI gene was cloned from soybean by RT-PCR.The GmAYI gene domain and the promoter element were analyzed.The results showed that the GmAYI gene of soybean was located on chromosome 4, which contained transmembrane domain and belonged to membrane protein.The promoter region of the gene contained a hormone-related response element and a cis-acting element necessary for endosperm expression. The results suggested that the gene may be involved in the regulation of soybean growth， development and environmental stress response process. The transcript levels of GmAYI gene in 14 soybean cultivars were quantitatively analyzed.Based on the correlation analysis of GmAYI gene transcripts in 14 soybean cultivars with seeds number per plant, seeds weight per plant, 100-seed weight and plant height, the expression of GmAYI gene was negatively correlated with 100-seed weight, suggested that the GmAYI gene may be involved in the regulation of soybean yield-related traits.

参考文献/References:

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备注/Memo

基金项目：黑龙江省博士后基金（LBH-Z12047）；抗逆转基因大豆新品种培育（2016ZX08004-002）；黑龙江省教育厅一般项目（10531012）。

第一作者简介：周淑莉（1990-），女，硕士，主要从事大豆基因克隆及功能验证等研究。E-mail：571729894@qq.com。
通讯作者：张彬彬（1977-），女，博士，副教授，主要从事大豆基因克隆、功能验证、转基因安全评价等方面研究工作。E-mail：bbzhang77@126.com。

更新日期/Last Update: 2016-12-09