[1]倪志勇,于月华,陈全家,等.大豆gma-miR1510a生物信息学分析及人工microRNA植物表达载体构建[J].大豆科学,2016,35(02):239-244.[doi:10.11861/j.issn.1000-9841.2016.02.0239]
　NI Zhi-yong,YU Yue-hua,CHEN Quan-jia,et al.Bioinformatic Analysis of gma-miR1510a and Construction of Its Artificial MiRNA Plant Expression Vectors[J].Soybean Science,2016,35(02):239-244.[doi:10.11861/j.issn.1000-9841.2016.02.0239]
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大豆gma-miR1510a生物信息学分析及人工microRNA植物表达载体构建

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年02期 页码: 239-244 栏目: 出版日期: 2016-03-20
Title:
Bioinformatic Analysis of gma-miR1510a and Construction of Its Artificial MiRNA Plant Expression Vectors
作者:
倪志勇; 于月华; 陈全家; 曲延英
新疆农业大学 农学院, 新疆 乌鲁木齐 830052
Author(s):
NI Zhi-yong;  YU Yue-hua;  CHEN Quan-jia;  QU Yan-ying
College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China
关键词:
大豆; gma-miR1510a; amiRNA; 启动子
Keywords:
Soybean;  gma-miR1510a;  amiRNA;  Promoter
DOI:
10.11861/j.issn.1000-9841.2016.02.0239
文献标志码:
A
摘要:
MicroRNAs (miRNAs)是最近发现的一类广泛存在的内生的小RNAs，在转录水平负调控基因的表达。本研究采用生物信息学方法分析gma-miR1510a的靶基因和启动子中的顺式作用元件。PLACE和PlantCARE启动子数据库分析表明gma-miR1510a启动子具有一些参与非生物胁迫、植物激素、组织特异表达和光应答元件。PMRD软件预测获得9个gma-miR1510a靶基因，属于抗病蛋白和ATP结合家族成员。通过重叠PCR方法替换拟南芥At-MIR319的成熟链和互补链序列，构建含有amiR1510a前体的植物表达载体amiR1510a-pCAMBIA3301。
Abstract:
Abstract:MicroRNAs (miRNAs) are newly discovered extensive endogenous small RNAs, which negatively regulate gene expression at the post-transcription levels.In this study，the target gene and the promoter of gmamiR1510a gene were predicted and analyzed by bioinformatics methodology.Many response elements for abiotic stress, plant hormones, tissue-specific expression and light were predicted by the database search programs PLACE and PlantCARE- PMRD software analysis showed that 9 transcripts were targeted by gma-miR1510a, these targets belonged to disease resistance protein (TIR-NBS-LRR class) and ATP binding.The amiR1510a sequence was engineered into the miRNA and miRNA* of At-MIR319 using overlapping PCR and the amiR1510a backbone was transferred into the plant expression vector pCAMBIA3301.

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备注/Memo

基金项目：中国博士后科学基金资助项目（2015M582741）；国家自然科学基金（31360264）；新疆维吾尔自治区高校科研计划科学研究重点项目（XJEDU2014I015）；新疆维吾尔自治区优秀博士后普通资助。

通讯作者：倪志勇（1981-），男，博士，副教授，主要从事植物逆境分子生物学研究。E-mail：nizhiyong@126.com。

更新日期/Last Update: 2016-04-04