[1]何庆元,向仕华,吴萍,等.硫化氢对盐胁迫条件下大豆抗氧化酶活性的影响[J].大豆科学,2015,34(03):427-431.[doi:10.11861/j.issn.1000-9841.2015.03.0427]
　HE Qing-yuan,XIANG Shi-hua,WU Ping,et al.Effects of Hydrogen Sulfide Alleviates Salt Stress in Soybean (Glycine max) Antioxidative System[J].Soybean Science,2015,34(03):427-431.[doi:10.11861/j.issn.1000-9841.2015.03.0427]
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硫化氢对盐胁迫条件下大豆抗氧化酶活性的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷 期数: 2015年03期 页码: 427-431 栏目: 出版日期: 2015-06-25
Title:
Effects of Hydrogen Sulfide Alleviates Salt Stress in Soybean (Glycine max) Antioxidative System
作者:
何庆元1; 2; 向仕华2; 3; 吴萍1; 李正鹏1; 王松华1; 祝嫦巍1; 张晓红1
1.安徽科技学院 生命科学学院，安徽 凤阳 233100；?
2.南京农业大学 大豆研究所/国家大豆改良中心/农业部大豆生物学与遗传育种与重点实验室/作物遗传与种质创新国家重点实验室, 江苏 南京 210095；?
3.自贡市农业科学研究所，四川 自贡 643000
Author(s):
HE Qing-yuan1; 2;  XIANG Shi-hua2; 3;  WU Ping1;  LI Zheng-peng1;  WANG Song-hua1;  ZHU Chang-wei1; ?ZHANG Xiao-hong1
1．Life Science College of Anhui Science and Technology University, Fengyang 233100, China; 2.Soybean Research Institute of Nanjing Agricultural University/National Center for Soybean Improvement/ Key Laboratory of Biology and Genetic Improvement of Soybean Mnistry of Agriculture P-R China/ National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, China; 3.Zigong Institute of Agricultural Sciences,Zigong 643000, China
关键词:
大豆; 硫化氢; NaCl胁迫; 缓解; 抗氧化酶
Keywords:
Glycine max;  Hydrogen sulfide;  NaCl stress;  Alleviation;  Antioxidative enzyme
DOI:
10.11861/j.issn.1000-9841.2015.03.0427
文献标志码:
A
摘要:
以南农1138-2为材料，采用不同浓度（0,0.20,0.40,0.60和0.80 mmol?L-1）的H2S供体NaHS溶液喷施在0.08 mol?L-1?的NaCl溶液胁迫下的大豆植株上，分析大豆从V2至V4期生长和同工酶活性的影响。结果表明：0.08 mol?L-1的NaCl溶液能有效抑制大豆生长。H2S的供体NaHS溶液喷施对盐胁迫有一定的缓解作用，0.20和0.40 mmol?L-1的NaHS处理的子叶等级显著高于阳性对照（0.00 mmol?L-1?NaHS处理），0.40 mmol?L-1的NaHS处理有最大的根冠比。0.40 mmol?L-1的NaHS处理下的V3和V4期大豆叶片中的过氧化物酶（POD）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性虽然比阴性对照低，但比其余处理都高，并都显著高于阳性对照。并且在V3期大豆的子叶等级与抗氧化酶活性成正相关关系。POD和SOD的同工酶在NaHS处理下谱带都发生了一定程度的改变。
Abstract:
Nannong1138.2 was used as test material, 0.08 mol?L-1NaCl stressed soybeans were sprayed different concentration NaHS (0, 0.20, 0.40, 0.60 and 0.80 mmol?L-1). The effects of growth and isozymes activity from V2 to V4 periods were analyzed by different treatments. The results showed that growth of soybean was inhibited by 0.08 mol?L-1?NaCl solutionH2S donor NaHS alleviates NaCl-induced stress. Treatments of 0.20 and 0.40 mmol?L-1?NaHS significantly increased the ranks of cotyledon than positive control and treatment of 0.40 mmol?L-1?NaHS had the highest ratio of root shoot.The activities of POD, SOD and CAT of treatment of 0.4 mmol?L-1?NaHS was lower than negative control but higher than others and significantly higher than positive control.The ranks of cotyledon were positively correlated with activities of antioxidative. NaHS treatments caused changes of the band number of POD and SOD.

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备注/Memo

基金项目：安徽教育厅重点项目（KJ2013A077）；四川省科技支撑计划（2015NZ0046）；安徽科技学院项目（ZRC2013383）。

第一作者简介：何庆元（1978-），男，博士，讲师，主要从事大豆遗传育种研究。E-mail:heqingyuan1@163.com。

更新日期/Last Update: 2015-07-15