[1]王朋,邓小娟,黄益安,等.大豆镉抗性及籽粒积累的根系基础研究[J].大豆科学,2014,33(06):928-931.[doi:10.11861/j.issn.1000-9841.2014.06.0928]
　WANG Peng,DENG Xiao-juan,HUANG Yian,et al.Roles of Soybean Roots in Tolerance to Cd and Seed Accumulation[J].Soybean Science,2014,33(06):928-931.[doi:10.11861/j.issn.1000-9841.2014.06.0928]
点击复制

大豆镉抗性及籽粒积累的根系基础研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第33卷 期数: 2014年06期 页码: 928-931 栏目: 出版日期: 2014-12-25
Title:
Roles of Soybean Roots in Tolerance to Cd and Seed Accumulation
文章编号:
1000-9841.2014.06.0928
作者:
王朋; 邓小娟; 黄益安; 方小龙; 张杰; 杨存义
华南农业大学 农学院/广东省植物分子育种重点实验室/国家大豆改良中心广东分中心，广东 广州 510640
Author(s):
WANG Peng; DENG Xiao-juan; HUANG Yian; FANG Xiao-long; ZHANG Jie; YANG Cun-yi
College of Agriculture，South China Agricultural University/Guangdong Provincial Key Laboratory of Plant Molecular Breeding/Subcenter of National Soybean Improvement Center,Guangzhou 510642,China
关键词:
大豆; 镉; 根系; 生理机制
Keywords:
Soybean; Cd ; Root; Physiological mechanism
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.06.0928
文献标志码:
A
摘要:
土壤镉（Cd）污染一方面会严重影响大豆的生长发育，另一方面通过土壤-根系积累在大豆籽粒中危及人体健康。选育高抗、低积累大豆品种是应对土壤镉污染的有效途径，了解大豆镉吸收、转运和分配的机制是开展大豆重金属高抗、低积累分子育种的前提。大豆根系是首先接触土壤的器官，也是镉吸收、转运和分配等重要生理活动发生的部位，因此是研究大豆镉抗性和积累的关注点。本文总结了大豆根系的形态、根系吸收、组织分配、固定去毒和木质部转运等方面在镉抗性和积累中所发挥的作用，通过比较不同大豆基因型根系在镉吸收、阻断、解毒和转运等方面的差异揭示了大豆镉抗性和积累机制的策略，为通过根系育种改良大豆抗污染及镉低积累的能力奠定基础。
Abstract:
Soybeans cultivation in the field contaminated with cadmium(Cd) faces two challenges:the first is the excessive soil cadmium will seriously impact soybean growth and development;the second is that Cd accumulation in seeds will threat the health of human being through the soil-root system.It is one of sustainable strategies to cultivate the tolerant and low accumulating soybean varieties in the field contaminated with Cd.Understanding the mechanism of uptake,translocation and contribution can help to improve the tolerance and accumulation.Soybean root is one of study focus because it is the organ interacted primarily with soil and play key roles in these processing,such as uptake,translocation and contribution.This article summarized the important roles of roots in cadmium absorption,cadmium detoxification,xylem transport,and also discussed the strategies which uncovered the mechanism of tolerance and low accumulation to cadmium by the comparison differences of roots in absorption,blocking,detoxification and transport between genotypes with contrast cadmium tolerance and accumulation.It may lay a foundation to improve the cadmium tolerance and low accumulation in soybean by root breeding.

参考文献/References:

［1］曾希柏，徐建明，黄巧云，等.中国农田重金属问题的若干思考[J].土壤学报,2013,50(1):186-194.( Zeng X B,Xu J M,Huang Q Y,et al.Some deliberations on the issues of heavy metals in farmlands of China[J].Journal of Soil,2013,50(1):186-194.)

[2]Zhao Y,Fang X,Mu Y,et al.Metal pollution(Cd,Pb,Zn and As)in agricultural soils and soybean,Glycine max,in Southern China[J].Bulletin of Environmental Contamation and Toxicology,2014,92(4):427-432.
[3]Zhuang P,Li Z A,Zou B,et al.Heavy metal contamination in soil and soybean near the Dabaoshan Mine,South China[J].Pedosphere,2013,23(3):298-304.
[4]Zhou H,Zeng M,Zhou X,et al.Assessment of heavy metal contamination and bioaccumulation in soybean plants from mining and smelting areas of southern Hunan province,China[J].Environmental Toxicology and Chemistry,2013,32(12):2719-2727.
[5]Grant C A,Clarke J M,Duguid S,et al.Selection and breeding of plant cultivars to minimize cadmium accumulation[J].Science of Total Environment,2008,390(2-3):301-310.
[6]赵云云，郭秀兰，钟彩霞，等.大豆抗镉污染低积累育种的研究进展[J].分子植物育种,2011(9):1692-1699.(Zhao Y Y,Guo X L,Zhong C X,et al.Advance in breeding of Cd-tolerant and low-accumulating soybean cultivars[J].Molecular Plant Breeding,2011(9):1692-1699.)
[7]赵云云，钟彩霞，方小龙，等.华南地区11个春播大豆品种耐镉性的差异[J].华南农业大学学报,2014,35(3):111-113.(Zhao Y Y,Zhong C X,Fang X L,et al.Genotypic differences of cadmium tolerance among spring-sowing soybean varieties in South China[J].Journal of South China Agricultural University,2014,35(3):111-113.)
[8]赵云云，钟彩霞，方小龙，等.华南地区夏播大豆品种镉耐性和籽粒镉积累的差异[J].大豆科学， 2013,34(3):336-340.(Zhao Y Y,Zhong C X,Fang X L,et al.Genotypic differences in tolerance and accumulation to Cd among summer-sowing soybean varieties in South China[J].Soybean Science,2013,34(3):336-340.)
[9]Boggess S F,Willavize S,Koeppe D E.Differential response of soybean varieties to soil cadmium[J].Agronomy Journal,1978,70(5):756-760.
[10]黄运湘，廖柏寒，王志坤，等.不同大豆品种镉毒害效应及耐镉差异性[J].湖南农业大学学报(自然科学版),2008,34(5):519-524.(Huang Y X,Liao B H,Wang Z K,et al.Cadmium toxicity and differences in tolerance to cadmium of various soybean varieties[J].Journal of Hunan Agricultural University,2008,34(5):519-524.)
[11] Arao T,Ae N,Sugiyama M,et al.Genotypic differences in cadmium uptake and distribution in soybeans[J].Japan Agricultural Research Quarterly,2003,251(2):247-253.
[12] Arao T I S.Genotypic differences in cadmium concentration and distribution of soybeans and rice[J].Japan Agricultural Research Quarterly,2006,40:21-30.
[13] Benitez E R,Hajika M,Yamada T,et al.A major QTL controlling seed cadmium accumulation in soybean[J].Crop Science,2010,50(5):1728-1734.
[14] Jegadeesan S,Yu K,Poysa V,et al.Mapping and validation of simple sequence repeat markers linked to a major gene controlling seed cadmium accumulation in soybean[J].Theoretical and Applied Genetics,2010,121(2):283-294.
[15] Ishikawa S,Ae N,Sugiyama M,et al.Genotypic variation in shoot cadmium concentration in rice and soybean in soils with different levels of cadmium contamination[J].Soil Science and Plant Nutrition,2005,51(1):101-108.
[16]Sugiyama M,Ae N,Arao T.Role of roots in differences in seed cadmium concentration among soybean cultivars-proof by grafting experiment[J].Plant and Soil,2007,295(1-2):1-11.
[17]Krzeslowska M.The cell wall in plant cell response to trace metals:polysaccharide remodeling and its role in defense strategy[J].Acta Physiologiae Plantarum,2011,33(1):35-51.
[18] Yang J L,Zhu X F,Peng Y X,et al.Cell wall hemicellulose contributes significantly to aluminum adsorption and root growth in Arabidopsis[J].Plant Physiology,2011,155(4):1885-1892.
[19] Cataldo D A,Garland T R,Wildung R E.Cadmium uptake kinetics in intact soybean plants[J].Plant Physiology,1983,73(3):844-848.
[20] Yang Y,Cheng L,Liu Z.Rapid effect of cadmium on lignin biosynthesis in soybean roots[J].Plant Science,2007,172(3):632-639.
[21] Finger-Teixeira A,Ferrarese Mde L,Soares A R,et al.Cadmium-induced lignification restricts soybean root growth[J].Ecotoxicology and Environmental Safty,2010,73(8):1959-1964.
[22] Liu J,Ma J,He C,et al.Inhibition of cadmium ion uptake in rice(Oryza sativa)cells by a wall-bound form of silicon[J].New Phytologist,2013,200(3):691-699.
[23] Zhu X F,Wang Z W,Dong F,et al.Exogenous auxin alleviates cadmium toxicity in Arabidopsis thaliana by stimulating synthesis of hemicellulose 1 and increasing the Cadmium fixation capacity of root cell walls[J].Journal of Hazard Materials,2013,263(2):398-403.
[24] Song Y F,Cui J,Zhang H X,et al.Proteomic analysis of copper stress responses in the roots of two rice(Oryza sativa L.)varieties differing in Cu tolerance[J].Plant and Soil,2013,366(1-2):647-658.
[25] Ahsan N,Nakamura T,Komatsu S.Differential responses of microsomal proteins and metabolites in two contrasting cadmium(Cd)accumulating soybean cultivars under Cd stress[J].Amino Acids,2012,42(1):317-327.
[26] Zhao Q,Nakashima J,Chen F,et al.Laccase is necessary and nonredundant with peroxidase for lignin polymerization during vascular development in Arabidopsis[J].Plant Cell,2013,25(10):3976-3987.
[27] Lux A,Martinka M,Vaculik M,et al.Root responses to cadmium in the rhizosphere:A review[J].Journal of Experimental Botany,2011,62(1):21-37.
[28]Li T,Di Z,Islam E,et al.Rhizosphere characteristics of zinc hyperaccumulator Sedum alfredii involved in zinc accumulation[J].Journal of Hazardous Materials,2011,185(2-3):818-823.
[29] Peng J,Gong J.Vacuolar sequestration capacity and long-distance metal transport in plants[J].Frontiers in Plant Science,2014,5:1-5.
[30 Pirselova B,Kuna R,Libantova J,et al.Biochemical and physiological comparison of heavy metal-triggered defense responses in the monocot maize and dicot soybean roots[J].Molecular Biology Reports,2011,38(5):3437-3446.
[31] Hossain Z,Nouri M Z,Komatsu S.Plant cell organelle proteomics in response to abiotic stress[J].Journal of Proteome Research,2012,11(1):37-48.
[32] Leszczyszyn O I,Imam H T,Blindauer C A.Diversity and distribution of plant metallothioneins:A review of structure,properties and functions[J].Metallomics,2013,5(9):1146-1169.
[33] Pagani M A,Tomas M,Carrillo J,et al.The response of the different soybean metallothionein isoforms to cadmium intoxication[J].Journal of Inorganic Biochemistry,2012,117:306-315.
[34] Peng J S,Gong J M.Vacuolar sequestration capacity and long-distance metal transport in plants[J].Frontiers in Plant Science,2014,5:19.
[35] Gill S S,Tuteja N.Cadmium stress tolerance in crop plants:Probing the role of sulfur[J].Plant Signaling & Behavior,2011,6(2):215-222.
[36] Pawlak-Sprada S,Arasimowicz-Jelonek M,Podgorska M,et al.Activation of phenylpropanoid pathway in legume plants exposed to heavy metals.Part I.Effects of cadmium and lead on phenylalanine ammonia-lyase gene expression,enzyme activity and lignin content[J].Acta Biochimica Polonica,2011,58(2):211-216.
[37] Pérez Chaca M V,Rodríguez Serrano M,Molina A S,et al.Cadmium induces two waves of reactive oxygen species in Glycine max(L.)roots[J].Plant,Cell & Environment,2014,37(7):1672-1687.
[38] Balestrasse K B,Yannarelli G G,Noriega G O,et al.Heme oxygenase and catalase gene expression in nodules and roots of soybean plants subjected to cadmium stress[J].BioMetals,2008,21(4):433-441.
[39] Ferreira R R,Fornazier R F,Vitoria A P,et al.Changes in antioxidant enzyme activities in soybean under cadmium stress[J].Journal of Plant Nutrition,2002,25(2):327-342.
[40] Hossain Z,Makino T,Komatsu S.Proteomic study of beta-aminobutyric acid-mediated cadmium stress alleviation in soybean[J].Journal of Proteomics,2012,75(13):4151-4164.
[41] Chmielowska-Bak J,Lefevre I,Lutts S,et al.Short term signaling responses in roots of young soybean seedlings exposed to cadmium stress[J].Journal of Plant Physiology,2013,170(18):1585-1594.
[42] Fang X,Zhao Y,Ma Q,et al.Identification and comparative analysis of cadmium toleranceassociated miRNAs and their targets in two soybean genotypes[J].PLoS One,2013,8(12):1-13.
[43]杨存义，黄兰兰，赵默然，等.miRNA 在植物适应土壤胁迫中的作用[J].中国农业科技导报,2010,12(1):16-22.(Yang C Y,Huang L L,Zhao M R,et al.Application and prospect of RNA interference in cotton roles of plant miRNA in adaptive responses to soil stresses[J].Journal of Agricultural Science and Technology,2010,12(1):16-22.)
[44] Gupta O P,Sharma P,Gupta R K,et al.MicroRNA mediated regulation of metal toxicity in plants:Present status and future perspectives[J].Plant Molecular Biology,2014,84(1-2):1-18.
[45] Kevresan S,Kirsek S,Kandrac J,et al.Dynamics of cadmium distribution in the intercellular space and inside cells in soybean roots,stems and leaves[J].Biologia Plantarum,2003,46(1):85-88.
[46] Cataldo D A,Garland T R,Wildung R E.Cadmium distribution and chemical fate in soybean plants[J].Plant Physiology,1981,68(4):835-839.
[47] Ohya T,Iikura H,Tanoi K,et al.-109-Cd uptake and translocation in a soybean plant under different pH conditions[J].Journal of Radioanalytical and Nuclear Chemistry,2005,264(2):303-306.
[48] Wang Y,Yu K F,Poysa V,et al.A single point mutation in GmHMA3 affects Cadmium(Cd)translocation and accumulation in soybean seeds[J].Molecular Plant,2012,5(5):1154-1156.

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(06):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(06):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(06):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(06):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(06):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(06):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(06):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(06):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(06):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(06):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]

备注/Memo

基金项目：国家自然科学基金（31271745）；国家“十二五”科技支撑计划（2011BAD35B06）；国家高技术研究发展计划“863计划”（2012AA101106）。

第一作者简介：王朋（1988-），男，硕士，主要从事大豆分子育种研究。E-mail:wp.102300@163.com。

更新日期/Last Update: 2014-12-29