ZHANG Ling,ZHANG Yuan-yu,LI Qi-yun,et al.The Research and Development Prospects of Soybean Seed Protein Expression System[J].Soybean Science,2014,33(04):616-618.[doi:10.11861/j.issn.1000-9841.2014.04.0616]
大豆种子蛋白表达系统的开发与研究前景
- Title:
- The Research and Development Prospects of Soybean Seed Protein Expression System
- Keywords:
- Soybean; Seed expression system; Recombinant protein
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 大豆种子中蛋白质含量高,是进行蛋白质鉴定的理想植物材料。利用转基因大豆来生产重组蛋白质具有生产成本低,纯化过程简单、技术含量低,规模化容易,蛋白质含量高、产品质量高,安全无污染的优点,并且大豆可以在温室条件下,各方面均优于细菌、酵母、动物细胞、转基因动物等生产体系。本文就通过转基因大豆种子表达系统生产外源蛋白的优点及在国内外的研究进展做一综述。
- Abstract:
- Soybean seed is an excellent protein producer,and also is the best material for protein identification and research.Expressing recombinant proteins by transgenic in soybean seeds possess many advantages,for example low cost of glycoslyated protein production,low-tech sustainability of purification lines,highest protein/biomass ratio,high product quality,reduced risk of contamination,minimal waste produced,as well as being a green technology.All aspects are better than bacteria,yeast,and animal protein expression technology.The merits of heterologous protein produced by transgenic soybean seed protein expression system and the progress in damestic and abroad were reviewed.
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备注/Memo
基金项目:转基因生物新品种培育重大专项(2013ZX08004)。