YANG Yu-ling,LIU Yuan-ying.Study on the Distribution of Selenium in Selenite-enrich Soybean[J].Soybean Science,2014,33(04):610-612.[doi:10.11861/j.issn.1000-9841.2014.04.0610]
富硒大豆中硒的分布研究
- Title:
- Study on the Distribution of Selenium in Selenite-enrich Soybean
- Keywords:
- Soybean; Selenium; Inorganic selenium; Organic selenium
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 通过叶面喷施亚硒酸钠对大豆进行富硒栽培,采用原子荧光法比较了不同硒浓度下毛豆各部位及成熟籽粒中总硒含量,并对大豆籽粒中无机硒和有机硒含量进行了比较研究。结果表明:大豆对硒具有良好的富集作用,硒含量顺序为:成熟籽粒>毛豆>毛豆叶>毛豆荚皮。在大豆中硒的主要存在形式是有机硒,约占总硒量的84%~91%,无机硒含量较少,仅占8.9%~16.0%。
- Abstract:
- Se-enriched soybean was prepared by foliar fertilization,the total selenium content of the different parts of edamame and mature soybean seeds were compared under different selenium concentration by atomic fluorescence spectrometry,and the inorganic selenium and organic selenium content in soybean seeds were studied.The results showed that the soybean had good effect on selenium enrichment,the order of selenium content was as follows:mature soybean seed>edamame>edamame leaf>edamame pod.In soybean grain selenium mainly existed in the form of organic selenium,which occupied by 84%-91% of total selenium content in soybean,inorganic selenium was relatively small,only account for 8.9% to 16.0%.
参考文献/References:
[1]Ellis D R,Salt D E.Plant,selenium and human health[J].Currrent Opinion in Plant Biology,2003,6:273-279. [2] Schomburg L,Schweizer U,KohrIe J.Selenium and selenoproteins in mammals :extrodinary,essential,enigmatic[J].CMLS,Cellular and Molecular Life Sciences,2004,61:1988-1995. [3] 胡秋辉,朱建春,潘根兴.土壤生态环境中硒的生物地球化学与食物链研究的最新进展[J].农村生态环境,2000,16(4):54-57.(Hu Q H,Zhu J C,Pan G X.Biological geochemistry and selenium in food chain[J].Rural Eco-Environment,2000,16(4):54-57.) [4] Mason A C,Weaver C M.Metabolism in rats of selenium from intrinsically and extrinsically labeled isolated soy protein[J].The Journal of Nutrition,1986,116:1883-1888. [5] Fang Y,Zhang Y F,Brittany Catron,et al.Identification of selenium compounds using HPLC-ICPMS and nano-ESI-MS in selenium-enriched rice viafoliar application[J].Journal of Analytical Atomic Spectrometry,2009,24:1657-1664. [6] 崔海容,陈建华,谷家越,等.顺序注射HG-AFS法测定富硒农产品中无机硒和有机硒[J].分析科学学报,2005,21(5):545-548.(Cui H R,Chen J H,Gu J Y,et al.Determination of organic and inorganic selenium in Se-rich farm products by sequence injection hydride generationatomic fluorescence spectrometry[J].Journal of Analytical Science,2005,21(5):545-548.) [7] 莫海珍.高有机硒保存率蔬菜富集和加工机理研究[D].无锡:江南大学,2007.(Mo H Z.Selenium enrichment in veaetables and the effects of processing parameters[D].Wu Xi:Jiangnan University,2007.) [8] Schrauzer G N.Anticarcinogenic effects of selenium[J].Cellular and Molecular Life Sciences,2000,57,1864-1873. [9] Gangher H E,Levander O A,Baumann C A.Dietary control of selenium volatilization in rat[J].Journal of Nutrition,1966,88,55-60. [10]Chan Q L,Caruso J A.A metallomics approach discovers selenium-containing proteins in selenium-enriched soybean[J].Analytical and Bioanalytical Chemistry,(2012)403:1311-1321. [11]田俊梅,张丁,付瑞娟,等.大豆硒蛋白与亚硒酸钠生物利用的比较研究[J].大豆科学,2010,29(3):534-536.(Tian J M,Zang D,Fu R J,et al.Comparison of the bioavailability of soybean selenoprotein and sodium selenite[J].SoybeanScience,2010,29(3):534-536.)
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备注/Memo
基金项目:黑龙江省优势农产品生产与加工研发平台产业化项目(GJPT006-2)。